Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/46406 |
Resumo: | Copyright © 2019 Pacífico, Hilbert, Sofka, Dinhopl, Pap, Aspöck, Carriço and Hilbert. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samplesBacteriophagesUrineTracheal aspiratesBloodE. coliPeduovirusTunavirinaeTequintavirusCopyright © 2019 Pacífico, Hilbert, Sofka, Dinhopl, Pap, Aspöck, Carriço and Hilbert. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.The interaction between bacteriophages, bacteria and the human host as a tripartite system has recently captured attention. The taxonomic diversity of bacteriophages, as a natural parasite of bacteria, still remains obscure in human body biomes, representing a so-called "viral dark matter." Here, we isolated and characterized coliphages from blood, urine and tracheal aspirates samples collected at a tertiary care hospital in Austria. Phages were more often isolated from blood, followed by urine and tracheal aspirates. Phylogenetic analysis and genome comparisons allowed the identification of phages belonging to the Tunavirinae subfamily, and to the Peduovirus and Tequintavirus genera. Tunavirinae phages cluster together and are found in samples from 14 patients, suggesting their prevalence across a variety of human samples. When compared with other phage genomes, the highest similarity level was at 87.69% average nucleotide identity (ANI), which suggests that these are in fact a newly isolated phage species. Tequintavirus phages share a 95.90% with phage 3_29, challenging the ANI threshold currently accepted to differentiate phage species. The isolated phages appear to be virulent, with the exception of the Peduovirus members, which are integrative and seem to reside as prophages in bacterial genomes.FrontiersRepositório da Universidade de LisboaPacífico, CátiaHilbert, MiriamSofka, DmitrijDinhopl, NoraPap, Ildiko-JuliaAspöck, ChristophCarrico, Joao AndreHilbert, Friederike2021-02-17T17:03:15Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/46406engFront. Microbiol. 10:248410.3389/fmicb.2019.024841664-302Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:48:40Zoai:repositorio.ul.pt:10451/46406Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:58:35.122865Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples |
title |
Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples |
spellingShingle |
Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples Pacífico, Cátia Bacteriophages Urine Tracheal aspirates Blood E. coli Peduovirus Tunavirinae Tequintavirus |
title_short |
Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples |
title_full |
Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples |
title_fullStr |
Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples |
title_full_unstemmed |
Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples |
title_sort |
Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples |
author |
Pacífico, Cátia |
author_facet |
Pacífico, Cátia Hilbert, Miriam Sofka, Dmitrij Dinhopl, Nora Pap, Ildiko-Julia Aspöck, Christoph Carrico, Joao Andre Hilbert, Friederike |
author_role |
author |
author2 |
Hilbert, Miriam Sofka, Dmitrij Dinhopl, Nora Pap, Ildiko-Julia Aspöck, Christoph Carrico, Joao Andre Hilbert, Friederike |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Pacífico, Cátia Hilbert, Miriam Sofka, Dmitrij Dinhopl, Nora Pap, Ildiko-Julia Aspöck, Christoph Carrico, Joao Andre Hilbert, Friederike |
dc.subject.por.fl_str_mv |
Bacteriophages Urine Tracheal aspirates Blood E. coli Peduovirus Tunavirinae Tequintavirus |
topic |
Bacteriophages Urine Tracheal aspirates Blood E. coli Peduovirus Tunavirinae Tequintavirus |
description |
Copyright © 2019 Pacífico, Hilbert, Sofka, Dinhopl, Pap, Aspöck, Carriço and Hilbert. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z 2021-02-17T17:03:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/46406 |
url |
http://hdl.handle.net/10451/46406 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Front. Microbiol. 10:2484 10.3389/fmicb.2019.02484 1664-302X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers |
publisher.none.fl_str_mv |
Frontiers |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134531758850048 |