Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2020
Outros Autores: Dias, Marta, Carolino, Elisabete, Sabino, Raquel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/12532
Resumo: Project EXPOsE. Project nº 23222 (02/SAICT/2016).
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spelling Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidenceOccupational healthOccupational exposureAssessment exposureMalt extract agarDG18SamplingIndoorAspergillus sppProject EXPOsEProject nº 23222 (02/SAICT/2016)Project Waste FRPDProject IPL/2018/WasteFRPD_ESTeSLProject Occupational exposure of ambulance drivers to bioburdenProject IPL/2020/BIO-AmbuDrivers_ESTeSLFCT_UIDB/05608/2020FCT_UIDP/05608/2020Project EXPOsE. Project nº 23222 (02/SAICT/2016).Project Waste FRPD. Project IPL/2018/WasteFRPD_ESTeSL.Project Occupational exposure of ambulance drivers to bioburden. Project IPL/2020/BIO-AmbuDrivers_ESTeSL.FCT_UIDB/05608/2020. FCT_UIDP/05608/2020.Culturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4)). As such, the sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment.MDPIRCIPLViegas, CarlaDias, MartaCarolino, ElisabeteSabino, Raquel2020-12-29T15:20:27Z2020-122020-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/12532engViegas C, Dias M, Carolino E, Sabino R. Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence. Atmosphere. 2021;12:23.10.3390/atmos12010023info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:05:36Zoai:repositorio.ipl.pt:10400.21/12532Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:20:36.218781Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence
title Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence
spellingShingle Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence
Viegas, Carla
Occupational health
Occupational exposure
Assessment exposure
Malt extract agar
DG18
Sampling
Indoor
Aspergillus spp
Project EXPOsE
Project nº 23222 (02/SAICT/2016)
Project Waste FRPD
Project IPL/2018/WasteFRPD_ESTeSL
Project Occupational exposure of ambulance drivers to bioburden
Project IPL/2020/BIO-AmbuDrivers_ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
title_short Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence
title_full Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence
title_fullStr Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence
title_full_unstemmed Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence
title_sort Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence
author Viegas, Carla
author_facet Viegas, Carla
Dias, Marta
Carolino, Elisabete
Sabino, Raquel
author_role author
author2 Dias, Marta
Carolino, Elisabete
Sabino, Raquel
author2_role author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Viegas, Carla
Dias, Marta
Carolino, Elisabete
Sabino, Raquel
dc.subject.por.fl_str_mv Occupational health
Occupational exposure
Assessment exposure
Malt extract agar
DG18
Sampling
Indoor
Aspergillus spp
Project EXPOsE
Project nº 23222 (02/SAICT/2016)
Project Waste FRPD
Project IPL/2018/WasteFRPD_ESTeSL
Project Occupational exposure of ambulance drivers to bioburden
Project IPL/2020/BIO-AmbuDrivers_ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
topic Occupational health
Occupational exposure
Assessment exposure
Malt extract agar
DG18
Sampling
Indoor
Aspergillus spp
Project EXPOsE
Project nº 23222 (02/SAICT/2016)
Project Waste FRPD
Project IPL/2018/WasteFRPD_ESTeSL
Project Occupational exposure of ambulance drivers to bioburden
Project IPL/2020/BIO-AmbuDrivers_ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
description Project EXPOsE. Project nº 23222 (02/SAICT/2016).
publishDate 2020
dc.date.none.fl_str_mv 2020-12-29T15:20:27Z
2020-12
2020-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/12532
url http://hdl.handle.net/10400.21/12532
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Viegas C, Dias M, Carolino E, Sabino R. Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence. Atmosphere. 2021;12:23.
10.3390/atmos12010023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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