Assessment of neuronal cytotoxicity of JWH-073 and JWH-250

Detalhes bibliográficos
Autor(a) principal: Pereira, Carlos Victor Montefusco
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.26/6715
Resumo: Dissertação de Erasmus Mundus para obtenção do grau de mestre em Técnicas Laboratoriais Forenses
id RCAP_ac6934039fd36bb9d389ec8df56962ca
oai_identifier_str oai:comum.rcaap.pt:10400.26/6715
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Assessment of neuronal cytotoxicity of JWH-073 and JWH-250Synthetic cannabinoidsForensic toxicologyIllicit drugsNapthtoylindolesPhenylacetylindolesMarijuanaDissertação de Erasmus Mundus para obtenção do grau de mestre em Técnicas Laboratoriais ForensesSynthetic cannabinoids from marijuana herbal blends like ‘Spice’ and ‘K2’ are drawing the attention of drug of abuse organizations, including the UNODC1, the EMCCDA2 and emergency hospital all over the world. This concern rises from clinical episodes of psychotropic effects that go beyond the regular range of marijuana and THC – namely, panic attacks, psychosis, catatonia, addiction and withdrawal symptoms. Our study addressed two emergent synthetic cannabinoids (napthtoylindoles) denominated JWH-073 and JWH-250 that are currently detected on ‘Spice’-like products, in order to observe their cell toxicity profile on neuronal cells in vitro model (SH-SY5Y). Using 0.2% DMSO as negative control, MTT and LDH results revealed that within concentrations of 1, 5, 10, 25, 37.5 and 50 μM, JWH-250 is identified as ‘toxic’ in a statistically significant manner at higher concentrations. This work did not detect any statistically significant toxicity from JWH-073. This data suggests to extend these studies on new synthetic cannabinoids to neuronal cells with increased concentrations, as well as the application of assays assessing apoptosis (conditions and signalling), neuronal function and activity (as cell membrane potential assay) within differentiated cells as neurons and glia. At the same time, the evaluation of herbal mixtures of more than one cannabinoids and plant types is advisable in order to understand synergic effects.EACEA - European CommissionInstituto Superior de Ciências da Saúde Egas MonizQuintas, AlexandreCosta, Joana Couceiro daRepositório ComumPereira, Carlos Victor Montefusco2014-09-18T11:41:18Z2014-07-01T00:00:00Z2014-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.26/6715201541491enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-06T14:51:29Zoai:comum.rcaap.pt:10400.26/6715Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:05.542897Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Assessment of neuronal cytotoxicity of JWH-073 and JWH-250
title Assessment of neuronal cytotoxicity of JWH-073 and JWH-250
spellingShingle Assessment of neuronal cytotoxicity of JWH-073 and JWH-250
Pereira, Carlos Victor Montefusco
Synthetic cannabinoids
Forensic toxicology
Illicit drugs
Napthtoylindoles
Phenylacetylindoles
Marijuana
title_short Assessment of neuronal cytotoxicity of JWH-073 and JWH-250
title_full Assessment of neuronal cytotoxicity of JWH-073 and JWH-250
title_fullStr Assessment of neuronal cytotoxicity of JWH-073 and JWH-250
title_full_unstemmed Assessment of neuronal cytotoxicity of JWH-073 and JWH-250
title_sort Assessment of neuronal cytotoxicity of JWH-073 and JWH-250
author Pereira, Carlos Victor Montefusco
author_facet Pereira, Carlos Victor Montefusco
author_role author
dc.contributor.none.fl_str_mv Quintas, Alexandre
Costa, Joana Couceiro da
Repositório Comum
dc.contributor.author.fl_str_mv Pereira, Carlos Victor Montefusco
dc.subject.por.fl_str_mv Synthetic cannabinoids
Forensic toxicology
Illicit drugs
Napthtoylindoles
Phenylacetylindoles
Marijuana
topic Synthetic cannabinoids
Forensic toxicology
Illicit drugs
Napthtoylindoles
Phenylacetylindoles
Marijuana
description Dissertação de Erasmus Mundus para obtenção do grau de mestre em Técnicas Laboratoriais Forenses
publishDate 2014
dc.date.none.fl_str_mv 2014-09-18T11:41:18Z
2014-07-01T00:00:00Z
2014-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/6715
201541491
url http://hdl.handle.net/10400.26/6715
identifier_str_mv 201541491
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Superior de Ciências da Saúde Egas Moniz
publisher.none.fl_str_mv Instituto Superior de Ciências da Saúde Egas Moniz
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799129931451465728

Registros relacionados