Unravelling the Protective Mechanisms Induced by Mucosal Immunization Against Neosporosis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/17526 |
Resumo: | Neospora caninum is an intercellular parasite causatory of aggraveted monetery loss in the bovine industry. Although vaccination is the most cost-effective prevention strategy, no commercial vaccine is available. An intranasal vaccination strategy using membrane proteins formulated with CpG has show to be protective against N. caninum infection in mice. This study aimed to identify the main protective machanisms induce by immunization. The involvement of different cell populations responding to IFN-y in the immune protection against the parasite was assessed. Mice in which myeloid cells do not express the IFN-y receptor were more susceptible to neosporosis, yet more resistant than IFN-y-/- mice. Also, increased parasite burdens were observed in mice with endothelial cells unable to respond to IFn-y. The role of IL-17 in resistance to neosporosis was evaluated. Il-17 neutrlization in sham-vacinated mice did not change the parasite burdens, however, in vaccinated mice, IL-17 neutralization led to decreased parasite burden, suggesting that this cytokine could have a deleterious effect on resistance against N. caninum infection. These results evidence the importance of the IFN-y dependent activation of different cell populations in the control of N. caninum infection and identify myeloid and endothelial cells as important effectors in protection against neosporosis. |
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Unravelling the Protective Mechanisms Induced by Mucosal Immunization Against NeosporosisN. caninumEndothelial CellsGamma InterferonInterleukin 17Mucosal ImmunizationNeospora caninum is an intercellular parasite causatory of aggraveted monetery loss in the bovine industry. Although vaccination is the most cost-effective prevention strategy, no commercial vaccine is available. An intranasal vaccination strategy using membrane proteins formulated with CpG has show to be protective against N. caninum infection in mice. This study aimed to identify the main protective machanisms induce by immunization. The involvement of different cell populations responding to IFN-y in the immune protection against the parasite was assessed. Mice in which myeloid cells do not express the IFN-y receptor were more susceptible to neosporosis, yet more resistant than IFN-y-/- mice. Also, increased parasite burdens were observed in mice with endothelial cells unable to respond to IFn-y. The role of IL-17 in resistance to neosporosis was evaluated. Il-17 neutrlization in sham-vacinated mice did not change the parasite burdens, however, in vaccinated mice, IL-17 neutralization led to decreased parasite burden, suggesting that this cytokine could have a deleterious effect on resistance against N. caninum infection. These results evidence the importance of the IFN-y dependent activation of different cell populations in the control of N. caninum infection and identify myeloid and endothelial cells as important effectors in protection against neosporosis.Neospora caninum é um parasita intracelular obrigatório responsável por perdas monetárias avultadas na indústria do gado bovino. A vacinação é a medida mais eficaz contra este parasita, no entanto, não há vacina disponível no mercado. Uma estratégia de imunização intranasal usando proteínas de membrana e CpG como adjuvante mostrou ser protetora no modelo murino de infeção. Neste estudo, pretendeu-se identificar os mecanismos protetoras induzidos por essa imunização. Foi avaliada a influência da sinalização de IFN-y em diferentes populações celulares na proteção contra o parasita. Murganhos cujas células mieloides não expressavam o recetor de IFN-y foram mais suscetíveis à neosporose, porém mais resistentes do que murganhos IFN-y-/-. Um aumento na suscetibilidade foi também observado em murganhos com células endoteliais incapazes de responde a IFN-y. O papel da IL-17 na resistência à neosporose também avaliado. Não se verificaram diferenças na carga parasitária de murganhos não vacinados mas, e, murganhos vacinados, diminuiu a carga parasitária, sugerindo que esta citocina terá um efeito negativo na resistência à infeção por N. caninum. Estes resultados mostram a importância da ativação de várias populações celulares por IFN-y no controlo da neosporose e identificam as células mieloides e endoteliais como importantes para essa proteção.Correia, AlexandraResende, MarianaFerraz, RicardoRepositório Científico do Instituto Politécnico do PortoSilva, Ana Margarida Gomes Lobo da2023-12-22T01:31:12Z2020-12-222020-12-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.22/17526TID:202637522enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-27T01:48:32Zoai:recipp.ipp.pt:10400.22/17526Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:37:08.995703Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Unravelling the Protective Mechanisms Induced by Mucosal Immunization Against Neosporosis |
title |
Unravelling the Protective Mechanisms Induced by Mucosal Immunization Against Neosporosis |
spellingShingle |
Unravelling the Protective Mechanisms Induced by Mucosal Immunization Against Neosporosis Silva, Ana Margarida Gomes Lobo da N. caninum Endothelial Cells Gamma Interferon Interleukin 17 Mucosal Immunization |
title_short |
Unravelling the Protective Mechanisms Induced by Mucosal Immunization Against Neosporosis |
title_full |
Unravelling the Protective Mechanisms Induced by Mucosal Immunization Against Neosporosis |
title_fullStr |
Unravelling the Protective Mechanisms Induced by Mucosal Immunization Against Neosporosis |
title_full_unstemmed |
Unravelling the Protective Mechanisms Induced by Mucosal Immunization Against Neosporosis |
title_sort |
Unravelling the Protective Mechanisms Induced by Mucosal Immunization Against Neosporosis |
author |
Silva, Ana Margarida Gomes Lobo da |
author_facet |
Silva, Ana Margarida Gomes Lobo da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Correia, Alexandra Resende, Mariana Ferraz, Ricardo Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Silva, Ana Margarida Gomes Lobo da |
dc.subject.por.fl_str_mv |
N. caninum Endothelial Cells Gamma Interferon Interleukin 17 Mucosal Immunization |
topic |
N. caninum Endothelial Cells Gamma Interferon Interleukin 17 Mucosal Immunization |
description |
Neospora caninum is an intercellular parasite causatory of aggraveted monetery loss in the bovine industry. Although vaccination is the most cost-effective prevention strategy, no commercial vaccine is available. An intranasal vaccination strategy using membrane proteins formulated with CpG has show to be protective against N. caninum infection in mice. This study aimed to identify the main protective machanisms induce by immunization. The involvement of different cell populations responding to IFN-y in the immune protection against the parasite was assessed. Mice in which myeloid cells do not express the IFN-y receptor were more susceptible to neosporosis, yet more resistant than IFN-y-/- mice. Also, increased parasite burdens were observed in mice with endothelial cells unable to respond to IFn-y. The role of IL-17 in resistance to neosporosis was evaluated. Il-17 neutrlization in sham-vacinated mice did not change the parasite burdens, however, in vaccinated mice, IL-17 neutralization led to decreased parasite burden, suggesting that this cytokine could have a deleterious effect on resistance against N. caninum infection. These results evidence the importance of the IFN-y dependent activation of different cell populations in the control of N. caninum infection and identify myeloid and endothelial cells as important effectors in protection against neosporosis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-22 2020-12-22T00:00:00Z 2023-12-22T01:31:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/17526 TID:202637522 |
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http://hdl.handle.net/10400.22/17526 |
identifier_str_mv |
TID:202637522 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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