IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/202916 |
Resumo: | Chronic periodontitis (CP) is characterized by gingival inflammation and bone destruction. It has been reported that interferon-gamma (IFN-γ) levels are high in CP patients; however, the IFN-γ receptor (IFN-γR) has not been studied in gingival tissue from these patients. Objective: To evaluate IFN-γ levels and IFN-γR expression in gingival tissue biopsies from chronic periodontitis patients compared with healthy subjects (HS). Material and Methods: Gingival tissues were obtained from all study subjects, CP (n = 18) and healthy subjects (HS) (n = 12). A tissue section of each study subject was embedded in paraffin blocks to determine the expression of IFN-γ R (IFN-γR1 and IFN-γR2) through immunohistochemistry. Another section of the tissue was homogenized and IFN-γ was measured by the ELISA technique. Results: No significant differences were found in the IFN-γR1 expression within the cell layers of the gingival tissue of the study groups. When analyzing the IFN-γR2 expression it was found that IFN-γR2 is strongly expressed in the endothelial cells of CP patients when compared to HS (p<0.05). IFN-γ concentrations in the gingival tissue were significantly higher in CP patients than in HS. No significant correlation between IFN-γ levels and the expression of IFN-γR1 and IFN-γR2 was found. However, a positive correlation between IFN-γ levels and clinical parameters [probing depth (PD) and clinical attachment level (CAL)] was found. Conclusion: The study of IFN-γR expression in gingival tissue samples from patients with CP showed an increase only in the IFN-γR2 chain in endothelial cells when compared to HS. |
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Journal of applied oral science (Online) |
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IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitisInterferon-gamma receptor 2Interferon-gammaEndothelial cellsChronic periodontitisChronic periodontitis (CP) is characterized by gingival inflammation and bone destruction. It has been reported that interferon-gamma (IFN-γ) levels are high in CP patients; however, the IFN-γ receptor (IFN-γR) has not been studied in gingival tissue from these patients. Objective: To evaluate IFN-γ levels and IFN-γR expression in gingival tissue biopsies from chronic periodontitis patients compared with healthy subjects (HS). Material and Methods: Gingival tissues were obtained from all study subjects, CP (n = 18) and healthy subjects (HS) (n = 12). A tissue section of each study subject was embedded in paraffin blocks to determine the expression of IFN-γ R (IFN-γR1 and IFN-γR2) through immunohistochemistry. Another section of the tissue was homogenized and IFN-γ was measured by the ELISA technique. Results: No significant differences were found in the IFN-γR1 expression within the cell layers of the gingival tissue of the study groups. When analyzing the IFN-γR2 expression it was found that IFN-γR2 is strongly expressed in the endothelial cells of CP patients when compared to HS (p<0.05). IFN-γ concentrations in the gingival tissue were significantly higher in CP patients than in HS. No significant correlation between IFN-γ levels and the expression of IFN-γR1 and IFN-γR2 was found. However, a positive correlation between IFN-γ levels and clinical parameters [probing depth (PD) and clinical attachment level (CAL)] was found. Conclusion: The study of IFN-γR expression in gingival tissue samples from patients with CP showed an increase only in the IFN-γR2 chain in endothelial cells when compared to HS.Universidade de São Paulo. Faculdade de Odontologia de Bauru2022-09-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/202916Journal of Applied Oral Science; Vol. 26 (2018); e20170291Journal of Applied Oral Science; Vol. 26 (2018); e20170291Journal of Applied Oral Science; v. 26 (2018); e201702911678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/202916/186976Copyright (c) 2022 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFranco-Topete, RamónZepeda-Nuño, José SergioZamora-Perez, Ana LourdesFuentes-Lerma, Martha GracielaGuerrero-Velázquez, Celia2022-09-26T17:33:41Zoai:revistas.usp.br:article/202916Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2022-09-26T17:33:41Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis |
title |
IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis |
spellingShingle |
IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis Franco-Topete, Ramón Interferon-gamma receptor 2 Interferon-gamma Endothelial cells Chronic periodontitis |
title_short |
IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis |
title_full |
IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis |
title_fullStr |
IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis |
title_full_unstemmed |
IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis |
title_sort |
IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis |
author |
Franco-Topete, Ramón |
author_facet |
Franco-Topete, Ramón Zepeda-Nuño, José Sergio Zamora-Perez, Ana Lourdes Fuentes-Lerma, Martha Graciela Guerrero-Velázquez, Celia |
author_role |
author |
author2 |
Zepeda-Nuño, José Sergio Zamora-Perez, Ana Lourdes Fuentes-Lerma, Martha Graciela Guerrero-Velázquez, Celia |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Franco-Topete, Ramón Zepeda-Nuño, José Sergio Zamora-Perez, Ana Lourdes Fuentes-Lerma, Martha Graciela Guerrero-Velázquez, Celia |
dc.subject.por.fl_str_mv |
Interferon-gamma receptor 2 Interferon-gamma Endothelial cells Chronic periodontitis |
topic |
Interferon-gamma receptor 2 Interferon-gamma Endothelial cells Chronic periodontitis |
description |
Chronic periodontitis (CP) is characterized by gingival inflammation and bone destruction. It has been reported that interferon-gamma (IFN-γ) levels are high in CP patients; however, the IFN-γ receptor (IFN-γR) has not been studied in gingival tissue from these patients. Objective: To evaluate IFN-γ levels and IFN-γR expression in gingival tissue biopsies from chronic periodontitis patients compared with healthy subjects (HS). Material and Methods: Gingival tissues were obtained from all study subjects, CP (n = 18) and healthy subjects (HS) (n = 12). A tissue section of each study subject was embedded in paraffin blocks to determine the expression of IFN-γ R (IFN-γR1 and IFN-γR2) through immunohistochemistry. Another section of the tissue was homogenized and IFN-γ was measured by the ELISA technique. Results: No significant differences were found in the IFN-γR1 expression within the cell layers of the gingival tissue of the study groups. When analyzing the IFN-γR2 expression it was found that IFN-γR2 is strongly expressed in the endothelial cells of CP patients when compared to HS (p<0.05). IFN-γ concentrations in the gingival tissue were significantly higher in CP patients than in HS. No significant correlation between IFN-γ levels and the expression of IFN-γR1 and IFN-γR2 was found. However, a positive correlation between IFN-γ levels and clinical parameters [probing depth (PD) and clinical attachment level (CAL)] was found. Conclusion: The study of IFN-γR expression in gingival tissue samples from patients with CP showed an increase only in the IFN-γR2 chain in endothelial cells when compared to HS. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/202916 |
url |
https://www.revistas.usp.br/jaos/article/view/202916 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/202916/186976 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 26 (2018); e20170291 Journal of Applied Oral Science; Vol. 26 (2018); e20170291 Journal of Applied Oral Science; v. 26 (2018); e20170291 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221683265568768 |