Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods

Detalhes bibliográficos
Autor(a) principal: Mamede, A. P.
Data de Publicação: 2018
Outros Autores: Vassalo, A. R., Cunha, E., Gonçalves, D., Parker, S. F., Batista de Carvalho, L. A. E., Marques, M. P. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
DOI: 10.1039/C8RA05660A
Texto Completo: http://hdl.handle.net/10316/108034
https://doi.org/10.1039/C8RA05660A
Resumo: Two different deproteination and defatting processes of human bone were investigated, by combined infrared and neutron techniques: a previously reported hydrazine extraction and a newly developed multi-enzymatic treatment. Complementary Fourier transform infrared total attenuated reflectance and inelastic neutron scattering spectroscopies were applied, allowing access to all vibrational modes of the samples. The effectiveness of the different experimental protocols for removing the organic constituents of bone (lipids and protein) was probed, as well as their effect on bone's structural and crystallinity features. The results thus gathered are expected to have an impact on bioanthropological, archaeological and medical sciences, namely regarding the development of novel biocompatible materials for orthopaedic xenografts.
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spelling Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methodsTwo different deproteination and defatting processes of human bone were investigated, by combined infrared and neutron techniques: a previously reported hydrazine extraction and a newly developed multi-enzymatic treatment. Complementary Fourier transform infrared total attenuated reflectance and inelastic neutron scattering spectroscopies were applied, allowing access to all vibrational modes of the samples. The effectiveness of the different experimental protocols for removing the organic constituents of bone (lipids and protein) was probed, as well as their effect on bone's structural and crystallinity features. The results thus gathered are expected to have an impact on bioanthropological, archaeological and medical sciences, namely regarding the development of novel biocompatible materials for orthopaedic xenografts.Royal Society of Chemistry2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108034http://hdl.handle.net/10316/108034https://doi.org/10.1039/C8RA05660Aeng2046-2069Mamede, A. P.Vassalo, A. R.Cunha, E.Gonçalves, D.Parker, S. F.Batista de Carvalho, L. A. E.Marques, M. P. M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-07T10:34:00Zoai:estudogeral.uc.pt:10316/108034Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:24:18.305121Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
title Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
spellingShingle Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
Mamede, A. P.
Mamede, A. P.
title_short Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
title_full Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
title_fullStr Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
title_full_unstemmed Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
title_sort Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
author Mamede, A. P.
author_facet Mamede, A. P.
Mamede, A. P.
Vassalo, A. R.
Cunha, E.
Gonçalves, D.
Parker, S. F.
Batista de Carvalho, L. A. E.
Marques, M. P. M.
Vassalo, A. R.
Cunha, E.
Gonçalves, D.
Parker, S. F.
Batista de Carvalho, L. A. E.
Marques, M. P. M.
author_role author
author2 Vassalo, A. R.
Cunha, E.
Gonçalves, D.
Parker, S. F.
Batista de Carvalho, L. A. E.
Marques, M. P. M.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mamede, A. P.
Vassalo, A. R.
Cunha, E.
Gonçalves, D.
Parker, S. F.
Batista de Carvalho, L. A. E.
Marques, M. P. M.
description Two different deproteination and defatting processes of human bone were investigated, by combined infrared and neutron techniques: a previously reported hydrazine extraction and a newly developed multi-enzymatic treatment. Complementary Fourier transform infrared total attenuated reflectance and inelastic neutron scattering spectroscopies were applied, allowing access to all vibrational modes of the samples. The effectiveness of the different experimental protocols for removing the organic constituents of bone (lipids and protein) was probed, as well as their effect on bone's structural and crystallinity features. The results thus gathered are expected to have an impact on bioanthropological, archaeological and medical sciences, namely regarding the development of novel biocompatible materials for orthopaedic xenografts.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/108034
http://hdl.handle.net/10316/108034
https://doi.org/10.1039/C8RA05660A
url http://hdl.handle.net/10316/108034
https://doi.org/10.1039/C8RA05660A
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2046-2069
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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dc.identifier.doi.none.fl_str_mv 10.1039/C8RA05660A

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