Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi

Detalhes bibliográficos
Autor(a) principal: Ferreira, Patrick
Data de Publicação: 2023
Outros Autores: Chahed, Abdessalem, Estevinho, Leticia M., Seixas, Natália, Costa, Rodrigo, Choupina, Altino
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/28932
Resumo: Ink disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for ink disease in Portugal. P. cinnamomi is a highly aggressive and widely distributed plant pathogen, capable of infecting nearly 1000 host species. This oomycete causes substantial economic losses and is accountable for the decline of numerous plant species in Europe and worldwide. To date, no effective treatments are available to combat these pathogens. Given chestnut’s economic and ecological significance, particularly in Portugal, it is crucial to investigate the molecular mechanisms underlying the interaction between Phytophthora species and host plants. This can be achieved through the study of the glucanase inhibitor protein (GIP) produced by P. cinnamomi during infection. The technique of RNA interference (RNAi) was employed to suppress the GIP gene of P. cinnamomi. The resulting transformants, carrying the silenced gene, were used to infect C. sativa, allowing for the assessment of the effects of gene silencing on the plant’s phenotype. Additionally, bioinformatics tools predicted the secretion of GIP protein. The obtained results validate RNAi as a potential alternative tool for studying molecular factors and for controlling and managing P. cinnamomi.
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spelling Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomiChestnutPhytophthora cinnamomiInk diseaseGIPRNA interferenceSubcellular localizationInk disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for ink disease in Portugal. P. cinnamomi is a highly aggressive and widely distributed plant pathogen, capable of infecting nearly 1000 host species. This oomycete causes substantial economic losses and is accountable for the decline of numerous plant species in Europe and worldwide. To date, no effective treatments are available to combat these pathogens. Given chestnut’s economic and ecological significance, particularly in Portugal, it is crucial to investigate the molecular mechanisms underlying the interaction between Phytophthora species and host plants. This can be achieved through the study of the glucanase inhibitor protein (GIP) produced by P. cinnamomi during infection. The technique of RNA interference (RNAi) was employed to suppress the GIP gene of P. cinnamomi. The resulting transformants, carrying the silenced gene, were used to infect C. sativa, allowing for the assessment of the effects of gene silencing on the plant’s phenotype. Additionally, bioinformatics tools predicted the secretion of GIP protein. The obtained results validate RNAi as a potential alternative tool for studying molecular factors and for controlling and managing P. cinnamomi.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO UID/AGR/00690/2019MDPIBiblioteca Digital do IPBFerreira, PatrickChahed, AbdessalemEstevinho, Leticia M.Seixas, NatáliaCosta, RodrigoChoupina, Altino2023-12-13T12:32:11Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/28932engFerreira, Patrick; Chahed, Abdessalem; Estevinho, Leticia M.; Seixas, Natália; Costa, Rodrigo; Choupina, Altino (2023). Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomic. Plants. eISSN 2223-7747. 12:22, p. 1-1210.3390/plants122238212223-7747info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-20T01:18:47Zoai:bibliotecadigital.ipb.pt:10198/28932Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:55:04.609127Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi
title Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi
spellingShingle Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi
Ferreira, Patrick
Chestnut
Phytophthora cinnamomi
Ink disease
GIP
RNA interference
Subcellular localization
title_short Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi
title_full Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi
title_fullStr Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi
title_full_unstemmed Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi
title_sort Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi
author Ferreira, Patrick
author_facet Ferreira, Patrick
Chahed, Abdessalem
Estevinho, Leticia M.
Seixas, Natália
Costa, Rodrigo
Choupina, Altino
author_role author
author2 Chahed, Abdessalem
Estevinho, Leticia M.
Seixas, Natália
Costa, Rodrigo
Choupina, Altino
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Ferreira, Patrick
Chahed, Abdessalem
Estevinho, Leticia M.
Seixas, Natália
Costa, Rodrigo
Choupina, Altino
dc.subject.por.fl_str_mv Chestnut
Phytophthora cinnamomi
Ink disease
GIP
RNA interference
Subcellular localization
topic Chestnut
Phytophthora cinnamomi
Ink disease
GIP
RNA interference
Subcellular localization
description Ink disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for ink disease in Portugal. P. cinnamomi is a highly aggressive and widely distributed plant pathogen, capable of infecting nearly 1000 host species. This oomycete causes substantial economic losses and is accountable for the decline of numerous plant species in Europe and worldwide. To date, no effective treatments are available to combat these pathogens. Given chestnut’s economic and ecological significance, particularly in Portugal, it is crucial to investigate the molecular mechanisms underlying the interaction between Phytophthora species and host plants. This can be achieved through the study of the glucanase inhibitor protein (GIP) produced by P. cinnamomi during infection. The technique of RNA interference (RNAi) was employed to suppress the GIP gene of P. cinnamomi. The resulting transformants, carrying the silenced gene, were used to infect C. sativa, allowing for the assessment of the effects of gene silencing on the plant’s phenotype. Additionally, bioinformatics tools predicted the secretion of GIP protein. The obtained results validate RNAi as a potential alternative tool for studying molecular factors and for controlling and managing P. cinnamomi.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-13T12:32:11Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/28932
url http://hdl.handle.net/10198/28932
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ferreira, Patrick; Chahed, Abdessalem; Estevinho, Leticia M.; Seixas, Natália; Costa, Rodrigo; Choupina, Altino (2023). Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomic. Plants. eISSN 2223-7747. 12:22, p. 1-12
10.3390/plants12223821
2223-7747
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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