Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/28932 |
Resumo: | Ink disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for ink disease in Portugal. P. cinnamomi is a highly aggressive and widely distributed plant pathogen, capable of infecting nearly 1000 host species. This oomycete causes substantial economic losses and is accountable for the decline of numerous plant species in Europe and worldwide. To date, no effective treatments are available to combat these pathogens. Given chestnut’s economic and ecological significance, particularly in Portugal, it is crucial to investigate the molecular mechanisms underlying the interaction between Phytophthora species and host plants. This can be achieved through the study of the glucanase inhibitor protein (GIP) produced by P. cinnamomi during infection. The technique of RNA interference (RNAi) was employed to suppress the GIP gene of P. cinnamomi. The resulting transformants, carrying the silenced gene, were used to infect C. sativa, allowing for the assessment of the effects of gene silencing on the plant’s phenotype. Additionally, bioinformatics tools predicted the secretion of GIP protein. The obtained results validate RNAi as a potential alternative tool for studying molecular factors and for controlling and managing P. cinnamomi. |
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Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomiChestnutPhytophthora cinnamomiInk diseaseGIPRNA interferenceSubcellular localizationInk disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for ink disease in Portugal. P. cinnamomi is a highly aggressive and widely distributed plant pathogen, capable of infecting nearly 1000 host species. This oomycete causes substantial economic losses and is accountable for the decline of numerous plant species in Europe and worldwide. To date, no effective treatments are available to combat these pathogens. Given chestnut’s economic and ecological significance, particularly in Portugal, it is crucial to investigate the molecular mechanisms underlying the interaction between Phytophthora species and host plants. This can be achieved through the study of the glucanase inhibitor protein (GIP) produced by P. cinnamomi during infection. The technique of RNA interference (RNAi) was employed to suppress the GIP gene of P. cinnamomi. The resulting transformants, carrying the silenced gene, were used to infect C. sativa, allowing for the assessment of the effects of gene silencing on the plant’s phenotype. Additionally, bioinformatics tools predicted the secretion of GIP protein. The obtained results validate RNAi as a potential alternative tool for studying molecular factors and for controlling and managing P. cinnamomi.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO UID/AGR/00690/2019MDPIBiblioteca Digital do IPBFerreira, PatrickChahed, AbdessalemEstevinho, Leticia M.Seixas, NatáliaCosta, RodrigoChoupina, Altino2023-12-13T12:32:11Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/28932engFerreira, Patrick; Chahed, Abdessalem; Estevinho, Leticia M.; Seixas, Natália; Costa, Rodrigo; Choupina, Altino (2023). Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomic. Plants. eISSN 2223-7747. 12:22, p. 1-1210.3390/plants122238212223-7747info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-20T01:18:47Zoai:bibliotecadigital.ipb.pt:10198/28932Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:55:04.609127Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi |
title |
Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi |
spellingShingle |
Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi Ferreira, Patrick Chestnut Phytophthora cinnamomi Ink disease GIP RNA interference Subcellular localization |
title_short |
Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi |
title_full |
Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi |
title_fullStr |
Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi |
title_full_unstemmed |
Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi |
title_sort |
Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomi |
author |
Ferreira, Patrick |
author_facet |
Ferreira, Patrick Chahed, Abdessalem Estevinho, Leticia M. Seixas, Natália Costa, Rodrigo Choupina, Altino |
author_role |
author |
author2 |
Chahed, Abdessalem Estevinho, Leticia M. Seixas, Natália Costa, Rodrigo Choupina, Altino |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Ferreira, Patrick Chahed, Abdessalem Estevinho, Leticia M. Seixas, Natália Costa, Rodrigo Choupina, Altino |
dc.subject.por.fl_str_mv |
Chestnut Phytophthora cinnamomi Ink disease GIP RNA interference Subcellular localization |
topic |
Chestnut Phytophthora cinnamomi Ink disease GIP RNA interference Subcellular localization |
description |
Ink disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for ink disease in Portugal. P. cinnamomi is a highly aggressive and widely distributed plant pathogen, capable of infecting nearly 1000 host species. This oomycete causes substantial economic losses and is accountable for the decline of numerous plant species in Europe and worldwide. To date, no effective treatments are available to combat these pathogens. Given chestnut’s economic and ecological significance, particularly in Portugal, it is crucial to investigate the molecular mechanisms underlying the interaction between Phytophthora species and host plants. This can be achieved through the study of the glucanase inhibitor protein (GIP) produced by P. cinnamomi during infection. The technique of RNA interference (RNAi) was employed to suppress the GIP gene of P. cinnamomi. The resulting transformants, carrying the silenced gene, were used to infect C. sativa, allowing for the assessment of the effects of gene silencing on the plant’s phenotype. Additionally, bioinformatics tools predicted the secretion of GIP protein. The obtained results validate RNAi as a potential alternative tool for studying molecular factors and for controlling and managing P. cinnamomi. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-13T12:32:11Z 2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/28932 |
url |
http://hdl.handle.net/10198/28932 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ferreira, Patrick; Chahed, Abdessalem; Estevinho, Leticia M.; Seixas, Natália; Costa, Rodrigo; Choupina, Altino (2023). Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophthora cinnamomic. Plants. eISSN 2223-7747. 12:22, p. 1-12 10.3390/plants12223821 2223-7747 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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