Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/158050 |
Resumo: | Synchrotron SAXS data were collected at beamline P12 operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). Publisher Copyright: © 2023 by the authors. |
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Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquidsencapsulationfluorinated ionic liquidsproteinsurface-active ionic liquidsPharmaceutical ScienceSynchrotron SAXS data were collected at beamline P12 operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). Publisher Copyright: © 2023 by the authors.Proteins are inherently unstable, which limits their use as therapeutic agents. However, the use of biocompatible cosolvents or surfactants can help to circumvent this problem through the stabilization of intramolecular and solvent-mediated interactions. Ionic liquids (ILs) have been known to act as cosolvents or surface-active compounds. In the presence of proteins, ILs can have a beneficial effect on their refolding, shelf life, stability, and enzymatic activities. In the work described herein, we used small-angle X-ray scattering (SAXS) to monitor the aggregation of different concentrations of ILs with protein models, lysozyme (Lys) and bovine serum albumin (BSA), and fluorescence microscopy to assess micelle formation of fluorinated ILs (FILs) with Lys. Furthermore, coarse-grained molecular dynamics (CG-MD) simulations provided a better understanding of Lys–FIL interactions. The results showed that the proteins maintain their globular structures in the presence of FILs, with signs of partial unfolding for Lys and compaction for BSA with increased flexibility at higher FIL concentrations. Lys was encapsulated by FIL, thus reinforcing the potential of ILs to be used in the formulation of protein-based pharmaceuticals.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)DQ - Departamento de QuímicaRUNAlves, Márcia M. S.Melo, Manuel N.Mertens, Haydyn D. T.Pereiro, Ana B.Archer, Margarida2023-09-20T22:18:01Z2023-01-032023-01-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20application/pdfhttp://hdl.handle.net/10362/158050eng1999-4923PURE: 72004508https://doi.org/10.3390/pharmaceutics15010157info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:14:31Zoai:run.unl.pt:10362/158050Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:14:31Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids |
| title |
Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids |
| spellingShingle |
Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids Alves, Márcia M. S. encapsulation fluorinated ionic liquids protein surface-active ionic liquids Pharmaceutical Science |
| title_short |
Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids |
| title_full |
Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids |
| title_fullStr |
Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids |
| title_full_unstemmed |
Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids |
| title_sort |
Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids |
| author |
Alves, Márcia M. S. |
| author_facet |
Alves, Márcia M. S. Melo, Manuel N. Mertens, Haydyn D. T. Pereiro, Ana B. Archer, Margarida |
| author_role |
author |
| author2 |
Melo, Manuel N. Mertens, Haydyn D. T. Pereiro, Ana B. Archer, Margarida |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Tecnologia Química e Biológica António Xavier (ITQB) DQ - Departamento de Química RUN |
| dc.contributor.author.fl_str_mv |
Alves, Márcia M. S. Melo, Manuel N. Mertens, Haydyn D. T. Pereiro, Ana B. Archer, Margarida |
| dc.subject.por.fl_str_mv |
encapsulation fluorinated ionic liquids protein surface-active ionic liquids Pharmaceutical Science |
| topic |
encapsulation fluorinated ionic liquids protein surface-active ionic liquids Pharmaceutical Science |
| description |
Synchrotron SAXS data were collected at beamline P12 operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). Publisher Copyright: © 2023 by the authors. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-09-20T22:18:01Z 2023-01-03 2023-01-03T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/158050 |
| url |
http://hdl.handle.net/10362/158050 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1999-4923 PURE: 72004508 https://doi.org/10.3390/pharmaceutics15010157 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
20 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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1817545956083630080 |