Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids

Detalhes bibliográficos
Autor(a) principal: Alves, Márcia M. S.
Data de Publicação: 2023
Outros Autores: Melo, Manuel N., Mertens, Haydyn D. T., Pereiro, Ana B., Archer, Margarida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158050
Resumo: Synchrotron SAXS data were collected at beamline P12 operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). Publisher Copyright: © 2023 by the authors.
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spelling Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquidsencapsulationfluorinated ionic liquidsproteinsurface-active ionic liquidsPharmaceutical ScienceSynchrotron SAXS data were collected at beamline P12 operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). Publisher Copyright: © 2023 by the authors.Proteins are inherently unstable, which limits their use as therapeutic agents. However, the use of biocompatible cosolvents or surfactants can help to circumvent this problem through the stabilization of intramolecular and solvent-mediated interactions. Ionic liquids (ILs) have been known to act as cosolvents or surface-active compounds. In the presence of proteins, ILs can have a beneficial effect on their refolding, shelf life, stability, and enzymatic activities. In the work described herein, we used small-angle X-ray scattering (SAXS) to monitor the aggregation of different concentrations of ILs with protein models, lysozyme (Lys) and bovine serum albumin (BSA), and fluorescence microscopy to assess micelle formation of fluorinated ILs (FILs) with Lys. Furthermore, coarse-grained molecular dynamics (CG-MD) simulations provided a better understanding of Lys–FIL interactions. The results showed that the proteins maintain their globular structures in the presence of FILs, with signs of partial unfolding for Lys and compaction for BSA with increased flexibility at higher FIL concentrations. Lys was encapsulated by FIL, thus reinforcing the potential of ILs to be used in the formulation of protein-based pharmaceuticals.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)DQ - Departamento de QuímicaRUNAlves, Márcia M. S.Melo, Manuel N.Mertens, Haydyn D. T.Pereiro, Ana B.Archer, Margarida2023-09-20T22:18:01Z2023-01-032023-01-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20application/pdfhttp://hdl.handle.net/10362/158050eng1999-4923PURE: 72004508https://doi.org/10.3390/pharmaceutics15010157info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:19Zoai:run.unl.pt:10362/158050Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:56:57.883540Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids
title Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids
spellingShingle Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids
Alves, Márcia M. S.
encapsulation
fluorinated ionic liquids
protein
surface-active ionic liquids
Pharmaceutical Science
title_short Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids
title_full Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids
title_fullStr Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids
title_full_unstemmed Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids
title_sort Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids
author Alves, Márcia M. S.
author_facet Alves, Márcia M. S.
Melo, Manuel N.
Mertens, Haydyn D. T.
Pereiro, Ana B.
Archer, Margarida
author_role author
author2 Melo, Manuel N.
Mertens, Haydyn D. T.
Pereiro, Ana B.
Archer, Margarida
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Alves, Márcia M. S.
Melo, Manuel N.
Mertens, Haydyn D. T.
Pereiro, Ana B.
Archer, Margarida
dc.subject.por.fl_str_mv encapsulation
fluorinated ionic liquids
protein
surface-active ionic liquids
Pharmaceutical Science
topic encapsulation
fluorinated ionic liquids
protein
surface-active ionic liquids
Pharmaceutical Science
description Synchrotron SAXS data were collected at beamline P12 operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-20T22:18:01Z
2023-01-03
2023-01-03T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158050
url http://hdl.handle.net/10362/158050
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1999-4923
PURE: 72004508
https://doi.org/10.3390/pharmaceutics15010157
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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