Tailor-made fluorinated ionic liquids for protein delivery

Detalhes bibliográficos
Autor(a) principal: Vieira, Nicole Sofia Morgado
Data de Publicação: 2020
Outros Autores: Castro, Paulo J., Marques, D. F., Araújo, João M. M., Pereiro, Ana Belén
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116222
Resumo: PTDC/QEQEPR/5841/2014 PTDC/QEQ-FTT/3289/2014 IF/00210/2014/CP1244/CT0003 UIDB/50006/2020
id RCAP_6cd29d0d395658314b3f49f33eea5184
oai_identifier_str oai:run.unl.pt:10362/116222
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Tailor-made fluorinated ionic liquids for protein deliveryAggregation behaviorBiological activityDelivery systemsEncapsulation efficiencyFluorinated ionic liquidsReleaseChemical Engineering(all)Materials Science(all)PTDC/QEQEPR/5841/2014 PTDC/QEQ-FTT/3289/2014 IF/00210/2014/CP1244/CT0003 UIDB/50006/2020Nowadays, pharmaceutical companies are facing several challenges with the development and approval of new biological products. The unique properties of several fluorinated ionic liquids (FILs), such as their high surfactant power in aqueous solutions, their chemical and biological stability, and low toxicity, favor their application in the pharmaceutical industry. Furthermore, the numerous combinations between cations and anions, in the FILs design, enlarge the possibilities to construct a successful delivery system. Several FILs also proved to not affect the activity, stability, and secondary structure of the therapeutic protein lysozyme. This work aims to study the aggregation behavior of distinct FILs in the protein suitable medium, in the presence or absence of lysozyme. Besides, different incubation conditions were tested to guarantee the optimal enzymatic activity of the protein at more stable delivery systems. Following the optimization of the incubation conditions, the quantification of the encapsulated lysozyme was performed to evaluate the encapsulation efficiency of each FIL-based system. The release of the protein was tested applying variables such as time, temperature, and ultrasound frequency. The experimental results suggest that the aggregation behavior of FILs is not significantly influenced by the protein and/or protein buffer and supports their application for the design of delivery systems with high encapsulation efficiencies, maintaining the biological activity of either encapsulated and released protein.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNVieira, Nicole Sofia MorgadoCastro, Paulo J.Marques, D. F.Araújo, João M. M.Pereiro, Ana Belén2021-04-26T22:58:42Z2020-08-142020-08-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://hdl.handle.net/10362/116222eng2079-4991PURE: 19798209https://doi.org/10.3390/nano10081594info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:58:41Zoai:run.unl.pt:10362/116222Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:59.849840Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Tailor-made fluorinated ionic liquids for protein delivery
title Tailor-made fluorinated ionic liquids for protein delivery
spellingShingle Tailor-made fluorinated ionic liquids for protein delivery
Vieira, Nicole Sofia Morgado
Aggregation behavior
Biological activity
Delivery systems
Encapsulation efficiency
Fluorinated ionic liquids
Release
Chemical Engineering(all)
Materials Science(all)
title_short Tailor-made fluorinated ionic liquids for protein delivery
title_full Tailor-made fluorinated ionic liquids for protein delivery
title_fullStr Tailor-made fluorinated ionic liquids for protein delivery
title_full_unstemmed Tailor-made fluorinated ionic liquids for protein delivery
title_sort Tailor-made fluorinated ionic liquids for protein delivery
author Vieira, Nicole Sofia Morgado
author_facet Vieira, Nicole Sofia Morgado
Castro, Paulo J.
Marques, D. F.
Araújo, João M. M.
Pereiro, Ana Belén
author_role author
author2 Castro, Paulo J.
Marques, D. F.
Araújo, João M. M.
Pereiro, Ana Belén
author2_role author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Vieira, Nicole Sofia Morgado
Castro, Paulo J.
Marques, D. F.
Araújo, João M. M.
Pereiro, Ana Belén
dc.subject.por.fl_str_mv Aggregation behavior
Biological activity
Delivery systems
Encapsulation efficiency
Fluorinated ionic liquids
Release
Chemical Engineering(all)
Materials Science(all)
topic Aggregation behavior
Biological activity
Delivery systems
Encapsulation efficiency
Fluorinated ionic liquids
Release
Chemical Engineering(all)
Materials Science(all)
description PTDC/QEQEPR/5841/2014 PTDC/QEQ-FTT/3289/2014 IF/00210/2014/CP1244/CT0003 UIDB/50006/2020
publishDate 2020
dc.date.none.fl_str_mv 2020-08-14
2020-08-14T00:00:00Z
2021-04-26T22:58:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116222
url http://hdl.handle.net/10362/116222
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2079-4991
PURE: 19798209
https://doi.org/10.3390/nano10081594
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138040536367104

Registros relacionados