Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal

Detalhes bibliográficos
Autor(a) principal: Costa, Sofia Santos
Data de Publicação: 2021
Outros Autores: Oliveira, Valéria, Serrano, Maria, Pomba, Constança, Couto, Isabel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/119234
Resumo: Funding Information: Funding: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade—COMPETE, by the Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through funds to GHTM (UID/04413/2020) and the CIISA Project (UID/CVT/00276/2020). Funding Information: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade?COMPETE, by the Funda??o para a Ci?ncia e a Tecnologia (FCT, Portugal)?Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through funds to GHTM (UID/04413/2020) and the CIISA Project (UID/CVT/00276/2020). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in PortugalMicrobiologyInfectious Diseasesveterinary(all)Molecular BiologySDG 3 - Good Health and Well-beingSDG 12 - Responsible Consumption and ProductionSDG 15 - Life on LandFunding Information: Funding: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade—COMPETE, by the Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through funds to GHTM (UID/04413/2020) and the CIISA Project (UID/CVT/00276/2020). Funding Information: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade?COMPETE, by the Funda??o para a Ci?ncia e a Tecnologia (FCT, Portugal)?Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through funds to GHTM (UID/04413/2020) and the CIISA Project (UID/CVT/00276/2020). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Staphylococcus coagulans is among the three most frequent pathogens of canine pyoderma. Yet, studies on this species are scarce. Twenty-seven S. coagulans and one S. schleiferi, corresponding to all pyoderma-related isolations from these two species at two veterinary laboratories in Lisbon, Portugal, between 1999 and 2018 (Lab 1) or 2018 (Lab 2), were analyzed. Isolates were identified by the analysis of the nuc gene and urease production. Antibiotic susceptibility towards 27 antibiotics was evaluated by disk diffusion. Fourteen antibiotic resistance genes were screened by PCR. Isolates were typed by SmaI-PFGE. Two S. coagulans isolates (2/27, 7.4%) were methicillin-resistant (MRSC, mecA+) and four (4/27, 14.8%) displayed a multidrug-resistant (MDR) phenotype. We observed resistance to penicillin (17/27, 63.0%), fluoroquinolones (11/27, 40.7%), erythromycin and clindamycin (3/27, 11.1%), fusidic acid (3/27, 11.1%) and tetracycline (1/27, 3.7%). The blaZ and erm(B) genes were carried by 16 and 1 isolates resistant to penicillin and erythromycin/clindamycin, respectively. Only three S. coagulans carried plasmids. The single S. schleiferi isolate presented an MDR phenotype. SmaI-PFGE revealed a limited genetic diversity of S. coagulans, with a predominant lineage present from 2001 to 2018. This study describes the first MRSC causing canine infection in Portugal and reveals a high burden of antimicrobial resistance, with the emergence of MDR phenotypes within the main lineages.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)TB, HIV and opportunistic diseases and pathogens (THOP)RUNCosta, Sofia SantosOliveira, ValériaSerrano, MariaPomba, ConstançaCouto, Isabel2021-06-14T22:16:14Z2021-05-022021-05-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/119234eng2079-6382PURE: 31529875https://doi.org/10.3390/antibiotics10050518info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:01:44Zoai:run.unl.pt:10362/119234Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:59.347587Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal
title Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal
spellingShingle Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal
Costa, Sofia Santos
Microbiology
Infectious Diseases
veterinary(all)
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 12 - Responsible Consumption and Production
SDG 15 - Life on Land
title_short Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal
title_full Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal
title_fullStr Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal
title_full_unstemmed Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal
title_sort Phenotypic and Molecular Traits of Staphylococcus coagulans Associated with Canine Skin Infections in Portugal
author Costa, Sofia Santos
author_facet Costa, Sofia Santos
Oliveira, Valéria
Serrano, Maria
Pomba, Constança
Couto, Isabel
author_role author
author2 Oliveira, Valéria
Serrano, Maria
Pomba, Constança
Couto, Isabel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Global Health and Tropical Medicine (GHTM)
TB, HIV and opportunistic diseases and pathogens (THOP)
RUN
dc.contributor.author.fl_str_mv Costa, Sofia Santos
Oliveira, Valéria
Serrano, Maria
Pomba, Constança
Couto, Isabel
dc.subject.por.fl_str_mv Microbiology
Infectious Diseases
veterinary(all)
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 12 - Responsible Consumption and Production
SDG 15 - Life on Land
topic Microbiology
Infectious Diseases
veterinary(all)
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 12 - Responsible Consumption and Production
SDG 15 - Life on Land
description Funding Information: Funding: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade—COMPETE, by the Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through funds to GHTM (UID/04413/2020) and the CIISA Project (UID/CVT/00276/2020). Funding Information: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade?COMPETE, by the Funda??o para a Ci?ncia e a Tecnologia (FCT, Portugal)?Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through funds to GHTM (UID/04413/2020) and the CIISA Project (UID/CVT/00276/2020). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-14T22:16:14Z
2021-05-02
2021-05-02T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/119234
url http://hdl.handle.net/10362/119234
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2079-6382
PURE: 31529875
https://doi.org/10.3390/antibiotics10050518
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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