Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/21348 |
Resumo: | Six strains of white-rot fungi isolated from southern Chile were evaluated for their ergosterol/biomass correlation and ligninolytic potential in solid medium to formulate pellets for Reactive Orange 165 (RO165) decolourization. The fungus Anthracophyllum discolor was selected to formulate complex pellets (fungal mycelium, sawdust, and activated carbon), coated pellets (complex pellet + alginate) and simple pellets (fungal mycelium). The activity of ligninolytic enzymes (laccase, manganese peroxidase, manganese-independent peroxidase, and lignin peroxidase) was evaluated in both the complex and coated pellets in modified Kirk medium, and the morphology of the pellets was studied using scanning electron microscopy (SEM). Complex pellets of A. discolor showed a higher enzymatic production mainly MnP (38 U L-1 at day 15) compared to coated and simple pellets. Examinations using SEM showed that both pellets produced a black core that was entrapped by a layer of fungal mycelium. Decolourization of RO165 was demonstrated with all the pellets formulated. However, the highest and fastest decolourization was obtained with complex pellets (100% at day 8). Therefore, complex pellets of A. discolor can be used for the biological treatment of wastewater contaminated with RO165. |
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Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourizationComplex pelletsLigninolytic enzymesRO 165 decolourizationWhite-rot fungiScience & TechnologySix strains of white-rot fungi isolated from southern Chile were evaluated for their ergosterol/biomass correlation and ligninolytic potential in solid medium to formulate pellets for Reactive Orange 165 (RO165) decolourization. The fungus Anthracophyllum discolor was selected to formulate complex pellets (fungal mycelium, sawdust, and activated carbon), coated pellets (complex pellet + alginate) and simple pellets (fungal mycelium). The activity of ligninolytic enzymes (laccase, manganese peroxidase, manganese-independent peroxidase, and lignin peroxidase) was evaluated in both the complex and coated pellets in modified Kirk medium, and the morphology of the pellets was studied using scanning electron microscopy (SEM). Complex pellets of A. discolor showed a higher enzymatic production mainly MnP (38 U L-1 at day 15) compared to coated and simple pellets. Examinations using SEM showed that both pellets produced a black core that was entrapped by a layer of fungal mycelium. Decolourization of RO165 was demonstrated with all the pellets formulated. However, the highest and fastest decolourization was obtained with complex pellets (100% at day 8). Therefore, complex pellets of A. discolor can be used for the biological treatment of wastewater contaminated with RO165.This research was supported by a FONDECYT grant 1090678 and a Doctoral thesis fellowship CONICYT 24100149.Pontificia Universidad Católica de ValparaísoUniversidade do MinhoElgueta, SebastianRubilar, OlgaLima, NelsonDiez, M. C.2012-11-152012-11-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/21348eng0717-345810.2225/vol15-issue6-fulltext-10http://dx.doi.org/10.2225/vol15-issue6-fulltext-10info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:15:28Zoai:repositorium.sdum.uminho.pt:1822/21348Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:07:54.089182Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
title |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
spellingShingle |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization Elgueta, Sebastian Complex pellets Ligninolytic enzymes RO 165 decolourization White-rot fungi Science & Technology |
title_short |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
title_full |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
title_fullStr |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
title_full_unstemmed |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
title_sort |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
author |
Elgueta, Sebastian |
author_facet |
Elgueta, Sebastian Rubilar, Olga Lima, Nelson Diez, M. C. |
author_role |
author |
author2 |
Rubilar, Olga Lima, Nelson Diez, M. C. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Elgueta, Sebastian Rubilar, Olga Lima, Nelson Diez, M. C. |
dc.subject.por.fl_str_mv |
Complex pellets Ligninolytic enzymes RO 165 decolourization White-rot fungi Science & Technology |
topic |
Complex pellets Ligninolytic enzymes RO 165 decolourization White-rot fungi Science & Technology |
description |
Six strains of white-rot fungi isolated from southern Chile were evaluated for their ergosterol/biomass correlation and ligninolytic potential in solid medium to formulate pellets for Reactive Orange 165 (RO165) decolourization. The fungus Anthracophyllum discolor was selected to formulate complex pellets (fungal mycelium, sawdust, and activated carbon), coated pellets (complex pellet + alginate) and simple pellets (fungal mycelium). The activity of ligninolytic enzymes (laccase, manganese peroxidase, manganese-independent peroxidase, and lignin peroxidase) was evaluated in both the complex and coated pellets in modified Kirk medium, and the morphology of the pellets was studied using scanning electron microscopy (SEM). Complex pellets of A. discolor showed a higher enzymatic production mainly MnP (38 U L-1 at day 15) compared to coated and simple pellets. Examinations using SEM showed that both pellets produced a black core that was entrapped by a layer of fungal mycelium. Decolourization of RO165 was demonstrated with all the pellets formulated. However, the highest and fastest decolourization was obtained with complex pellets (100% at day 8). Therefore, complex pellets of A. discolor can be used for the biological treatment of wastewater contaminated with RO165. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-15 2012-11-15T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/21348 |
url |
http://hdl.handle.net/1822/21348 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0717-3458 10.2225/vol15-issue6-fulltext-10 http://dx.doi.org/10.2225/vol15-issue6-fulltext-10 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pontificia Universidad Católica de Valparaíso |
publisher.none.fl_str_mv |
Pontificia Universidad Católica de Valparaíso |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132499546210304 |