A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4

Detalhes bibliográficos
Autor(a) principal: Acevedo,Francisca
Data de Publicação: 2011
Outros Autores: Pizzul,Leticia, Castillo,María del Pilar, Rubilar,Olga, Lienqueo,María Elena, Tortella,Gonzalo, Diez,María Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600013
Resumo: In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead . The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50ºC) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.
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spelling A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4white-rot fungiligninolytic enzymespurificationlignocellulosic materialIn this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead . The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50ºC) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.Instituto de Tecnologia do Paraná - Tecpar2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600013Brazilian Archives of Biology and Technology v.54 n.6 2011reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132011000600013info:eu-repo/semantics/openAccessAcevedo,FranciscaPizzul,LeticiaCastillo,María del PilarRubilar,OlgaLienqueo,María ElenaTortella,GonzaloDiez,María Cristinaeng2011-12-13T00:00:00Zoai:scielo:S1516-89132011000600013Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-12-13T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
title A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
spellingShingle A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
Acevedo,Francisca
white-rot fungi
ligninolytic enzymes
purification
lignocellulosic material
title_short A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
title_full A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
title_fullStr A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
title_full_unstemmed A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
title_sort A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
author Acevedo,Francisca
author_facet Acevedo,Francisca
Pizzul,Leticia
Castillo,María del Pilar
Rubilar,Olga
Lienqueo,María Elena
Tortella,Gonzalo
Diez,María Cristina
author_role author
author2 Pizzul,Leticia
Castillo,María del Pilar
Rubilar,Olga
Lienqueo,María Elena
Tortella,Gonzalo
Diez,María Cristina
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Acevedo,Francisca
Pizzul,Leticia
Castillo,María del Pilar
Rubilar,Olga
Lienqueo,María Elena
Tortella,Gonzalo
Diez,María Cristina
dc.subject.por.fl_str_mv white-rot fungi
ligninolytic enzymes
purification
lignocellulosic material
topic white-rot fungi
ligninolytic enzymes
purification
lignocellulosic material
description In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead . The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50ºC) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132011000600013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.54 n.6 2011
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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