Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54

Detalhes bibliográficos
Autor(a) principal: Fonseca, Fátima
Data de Publicação: 2011
Outros Autores: Arthur, Christopher J., Bromley, Elizabeth H. C., Samyn, Bart, Moerman, Pablo, Saavedra, Maria José, Correia, António, Spencer, James
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/25114
Resumo: The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and overexpressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties.
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spelling Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and overexpressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties.American Society for Microbiology2019-01-15T14:20:59Z2011-01-01T00:00:00Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/25114eng0066-480410.1128/AAC.00429-11Fonseca, FátimaArthur, Christopher J.Bromley, Elizabeth H. C.Samyn, BartMoerman, PabloSaavedra, Maria JoséCorreia, AntónioSpencer, Jamesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:48:16Zoai:ria.ua.pt:10773/25114Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:58:16.038952Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54
title Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54
spellingShingle Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54
Fonseca, Fátima
title_short Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54
title_full Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54
title_fullStr Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54
title_full_unstemmed Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54
title_sort Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54
author Fonseca, Fátima
author_facet Fonseca, Fátima
Arthur, Christopher J.
Bromley, Elizabeth H. C.
Samyn, Bart
Moerman, Pablo
Saavedra, Maria José
Correia, António
Spencer, James
author_role author
author2 Arthur, Christopher J.
Bromley, Elizabeth H. C.
Samyn, Bart
Moerman, Pablo
Saavedra, Maria José
Correia, António
Spencer, James
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca, Fátima
Arthur, Christopher J.
Bromley, Elizabeth H. C.
Samyn, Bart
Moerman, Pablo
Saavedra, Maria José
Correia, António
Spencer, James
description The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and overexpressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01T00:00:00Z
2011
2019-01-15T14:20:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/25114
url http://hdl.handle.net/10773/25114
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0066-4804
10.1128/AAC.00429-11
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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