Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/25114 |
Resumo: | The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and overexpressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties. |
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Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and overexpressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties.American Society for Microbiology2019-01-15T14:20:59Z2011-01-01T00:00:00Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/25114eng0066-480410.1128/AAC.00429-11Fonseca, FátimaArthur, Christopher J.Bromley, Elizabeth H. C.Samyn, BartMoerman, PabloSaavedra, Maria JoséCorreia, AntónioSpencer, Jamesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:48:16Zoai:ria.ua.pt:10773/25114Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:58:16.038952Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54 |
title |
Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54 |
spellingShingle |
Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54 Fonseca, Fátima |
title_short |
Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54 |
title_full |
Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54 |
title_fullStr |
Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54 |
title_full_unstemmed |
Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54 |
title_sort |
Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54 |
author |
Fonseca, Fátima |
author_facet |
Fonseca, Fátima Arthur, Christopher J. Bromley, Elizabeth H. C. Samyn, Bart Moerman, Pablo Saavedra, Maria José Correia, António Spencer, James |
author_role |
author |
author2 |
Arthur, Christopher J. Bromley, Elizabeth H. C. Samyn, Bart Moerman, Pablo Saavedra, Maria José Correia, António Spencer, James |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Fonseca, Fátima Arthur, Christopher J. Bromley, Elizabeth H. C. Samyn, Bart Moerman, Pablo Saavedra, Maria José Correia, António Spencer, James |
description |
The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and overexpressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01T00:00:00Z 2011 2019-01-15T14:20:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/25114 |
url |
http://hdl.handle.net/10773/25114 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0066-4804 10.1128/AAC.00429-11 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137637367283712 |