Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers

Detalhes bibliográficos
Autor(a) principal: Calejo, Isabel
Data de Publicação: 2022
Outros Autores: Reis, R. L., Domingues, Rui Miguel Andrade, Gomes, Manuela E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/75587
Resumo: "Online ahead of print"
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spelling Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibersEmulsion ElectrospinningFunctionally graded scaffoldsHierarchical tissuesPlatelet lysatesScience & Technology"Online ahead of print"The heterogeneity of the hierarchical tissues requires designing multipart engineered constructs as suitable tissue replacements. Herein, we propose the incorporation of platelet lysate (PL) within electrospun fiber core aiming the fabrication of functionally graded 3D scaffolds for heterotypic tissues regeneration, such as tendon-to-bone interface. First, anisotropic yarns (A-Yarns) and isotropic threads with nanohydroxyapatite (I-Threads/PL@nHAp) are fabricated to recreate the tendon- and bone- microstructures and both incorporated with PL using emulsion electrospinning for a sustained and local delivery of growth factors, cytokines, and chemokines. Biological performance using human adipose-derived stem cells demonstrates that A-Yarns/PL induce a higher expression of scleraxis, a tenogenic-marker, while in I-Threads/PL@nHAp, higher alkaline phosphatase activity and matrix mineralization suggest an osteogenic commitment without the need for external biochemical supplementation when compared to plain controls. As proof of concept, functional 3D gradient scaffolds are fabricated using weaving technique, resulting in 3D textured hierarchical constructs with gradients in composition and topography. Additionally, the precise delivery of bioactive cues together with in situ biophysical features guide the commitment into a phenotypic gradient exhibiting chondrogenic and osteochondrogenic profiles in the interface of scaffolds. Overall, we demonstrate a promising patch solution for the regeneration of tendon-to-bone tissue interface through the fabrication of PL-functional 3D gradient constructs.The authors would like to thank to Hospital da Prelada (Porto, Portugal) for the lipoaspirate tissue samples (Plastic Surgery Department). The authors acknowledge the financial support from the European Union Framework Programme for Research and Innovation HORIZON2020, Achilles (810850) and ERC CoG MagTendon (772817), and Fundacao para a Ciencia e a Tecnologia for MagTT (029930) and SmarTendon (PTDC/NAN-MAT/30595/2017) projects and the Ph.D. grant of IC (PD/BD/128088/2016).Wiley-VCH VerlagUniversidade do MinhoCalejo, IsabelReis, R. L.Domingues, Rui Miguel AndradeGomes, Manuela E.20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/75587engCalejo I., Reis R. L., Domingues R. M. A., Gomes M. E. Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers, Advanced Healthcare Materials, doi:10.1002/adhm.202102076, 20212192-26402192-265910.1002/adhm.20210207634927396https://doi.org/10.1002/adhm.202102076info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:14Zoai:repositorium.sdum.uminho.pt:1822/75587Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:56:51.084789Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers
title Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers
spellingShingle Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers
Calejo, Isabel
Emulsion Electrospinning
Functionally graded scaffolds
Hierarchical tissues
Platelet lysates
Science & Technology
title_short Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers
title_full Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers
title_fullStr Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers
title_full_unstemmed Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers
title_sort Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers
author Calejo, Isabel
author_facet Calejo, Isabel
Reis, R. L.
Domingues, Rui Miguel Andrade
Gomes, Manuela E.
author_role author
author2 Reis, R. L.
Domingues, Rui Miguel Andrade
Gomes, Manuela E.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Calejo, Isabel
Reis, R. L.
Domingues, Rui Miguel Andrade
Gomes, Manuela E.
dc.subject.por.fl_str_mv Emulsion Electrospinning
Functionally graded scaffolds
Hierarchical tissues
Platelet lysates
Science & Technology
topic Emulsion Electrospinning
Functionally graded scaffolds
Hierarchical tissues
Platelet lysates
Science & Technology
description "Online ahead of print"
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/75587
url https://hdl.handle.net/1822/75587
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Calejo I., Reis R. L., Domingues R. M. A., Gomes M. E. Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers, Advanced Healthcare Materials, doi:10.1002/adhm.202102076, 2021
2192-2640
2192-2659
10.1002/adhm.202102076
34927396
https://doi.org/10.1002/adhm.202102076
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH Verlag
publisher.none.fl_str_mv Wiley-VCH Verlag
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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