Hierarchical self-assembly of a reflectin-derived peptide
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/160874 |
Resumo: | Funding Information: Circular dichroism data were acquired at BioLab, UCIBIO FCT-NOVA. Mass spectrometry data were obtained by the mass spectrometry unit (UniMS), ITQB/iBET, Oeiras, Portugal. The authors would like to thank Ana Rita Duarte Lab from LAQV, REQUIMTE, FCT-NOVA for using the rheometer. The helpful discussion of the results with Rein Ulijn and Deborah Sementa and MSc. Maithreyi Ramakrishnan from ASRC-CUNY is also appreciated. The authors would like to acknowledge the financial support for AN master grant through the Erasmus + Program of the European Union. This work was produced with the support of INCD funded by FCT and FEDER under the project 01/SAICT/2016 no 02215. The authors would also like to thank the support of the Royal Society of Chemistry grant (R22-1009764370). Publisher Copyright: Copyright © 2023 Dias, Moreira, Lychko, Lopes Soares, Nurrito, Moura Barbosa, Lutz-Bueno, Mezzenga, Carvalho, Pina and Roque. |
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Hierarchical self-assembly of a reflectin-derived peptidebio-based materialsfilmshydrogelsoptical materialspeptidesreflectinsself-assemblysupramolecularChemistry(all)Funding Information: Circular dichroism data were acquired at BioLab, UCIBIO FCT-NOVA. Mass spectrometry data were obtained by the mass spectrometry unit (UniMS), ITQB/iBET, Oeiras, Portugal. The authors would like to thank Ana Rita Duarte Lab from LAQV, REQUIMTE, FCT-NOVA for using the rheometer. The helpful discussion of the results with Rein Ulijn and Deborah Sementa and MSc. Maithreyi Ramakrishnan from ASRC-CUNY is also appreciated. The authors would like to acknowledge the financial support for AN master grant through the Erasmus + Program of the European Union. This work was produced with the support of INCD funded by FCT and FEDER under the project 01/SAICT/2016 no 02215. The authors would also like to thank the support of the Royal Society of Chemistry grant (R22-1009764370). Publisher Copyright: Copyright © 2023 Dias, Moreira, Lychko, Lopes Soares, Nurrito, Moura Barbosa, Lutz-Bueno, Mezzenga, Carvalho, Pina and Roque.Reflectins are a family of intrinsically disordered proteins involved in cephalopod camouflage, making them an interesting source for bioinspired optical materials. Understanding reflectin assembly into higher-order structures by standard biophysical methods enables the rational design of new materials, but it is difficult due to their low solubility. To address this challenge, we aim to understand the molecular self-assembly mechanism of reflectin’s basic unit—the protopeptide sequence YMDMSGYQ—as a means to understand reflectin’s assembly phenomena. Protopeptide self-assembly was triggered by different environmental cues, yielding supramolecular hydrogels, and characterized by experimental and theoretical methods. Protopeptide films were also prepared to assess optical properties. Our results support the hypothesis for the protopeptide aggregation model at an atomistic level, led by hydrophilic and hydrophobic interactions mediated by tyrosine residues. Protopeptide-derived films were optically active, presenting diffuse reflectance in the visible region of the light spectrum. Hence, these results contribute to a better understanding of the protopeptide structural assembly, crucial for the design of peptide- and reflectin-based functional materials.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNDias, Ana Margarida Gonçalves CarvalhoMoreira, Inês PimentelLychko, IanaLopes Soares, CátiaNurrito, AriannaMoura Barbosa, Arménio JorgeLutz-Bueno, VivianeMezzenga, RaffaeleCarvalho, Ana LuísaPina, Ana SofiaRoque, Ana Cecília Afonso2023-12-04T22:34:46Z2023-09-212023-09-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/10362/160874eng2296-2646PURE: 77680214https://doi.org/10.3389/fchem.2023.1267563info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:43:38Zoai:run.unl.pt:10362/160874Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:58:15.193280Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Hierarchical self-assembly of a reflectin-derived peptide |
| title |
Hierarchical self-assembly of a reflectin-derived peptide |
| spellingShingle |
Hierarchical self-assembly of a reflectin-derived peptide Dias, Ana Margarida Gonçalves Carvalho bio-based materials films hydrogels optical materials peptides reflectins self-assembly supramolecular Chemistry(all) |
| title_short |
Hierarchical self-assembly of a reflectin-derived peptide |
| title_full |
Hierarchical self-assembly of a reflectin-derived peptide |
| title_fullStr |
Hierarchical self-assembly of a reflectin-derived peptide |
| title_full_unstemmed |
Hierarchical self-assembly of a reflectin-derived peptide |
| title_sort |
Hierarchical self-assembly of a reflectin-derived peptide |
| author |
Dias, Ana Margarida Gonçalves Carvalho |
| author_facet |
Dias, Ana Margarida Gonçalves Carvalho Moreira, Inês Pimentel Lychko, Iana Lopes Soares, Cátia Nurrito, Arianna Moura Barbosa, Arménio Jorge Lutz-Bueno, Viviane Mezzenga, Raffaele Carvalho, Ana Luísa Pina, Ana Sofia Roque, Ana Cecília Afonso |
| author_role |
author |
| author2 |
Moreira, Inês Pimentel Lychko, Iana Lopes Soares, Cátia Nurrito, Arianna Moura Barbosa, Arménio Jorge Lutz-Bueno, Viviane Mezzenga, Raffaele Carvalho, Ana Luísa Pina, Ana Sofia Roque, Ana Cecília Afonso |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química RUN |
| dc.contributor.author.fl_str_mv |
Dias, Ana Margarida Gonçalves Carvalho Moreira, Inês Pimentel Lychko, Iana Lopes Soares, Cátia Nurrito, Arianna Moura Barbosa, Arménio Jorge Lutz-Bueno, Viviane Mezzenga, Raffaele Carvalho, Ana Luísa Pina, Ana Sofia Roque, Ana Cecília Afonso |
| dc.subject.por.fl_str_mv |
bio-based materials films hydrogels optical materials peptides reflectins self-assembly supramolecular Chemistry(all) |
| topic |
bio-based materials films hydrogels optical materials peptides reflectins self-assembly supramolecular Chemistry(all) |
| description |
Funding Information: Circular dichroism data were acquired at BioLab, UCIBIO FCT-NOVA. Mass spectrometry data were obtained by the mass spectrometry unit (UniMS), ITQB/iBET, Oeiras, Portugal. The authors would like to thank Ana Rita Duarte Lab from LAQV, REQUIMTE, FCT-NOVA for using the rheometer. The helpful discussion of the results with Rein Ulijn and Deborah Sementa and MSc. Maithreyi Ramakrishnan from ASRC-CUNY is also appreciated. The authors would like to acknowledge the financial support for AN master grant through the Erasmus + Program of the European Union. This work was produced with the support of INCD funded by FCT and FEDER under the project 01/SAICT/2016 no 02215. The authors would also like to thank the support of the Royal Society of Chemistry grant (R22-1009764370). Publisher Copyright: Copyright © 2023 Dias, Moreira, Lychko, Lopes Soares, Nurrito, Moura Barbosa, Lutz-Bueno, Mezzenga, Carvalho, Pina and Roque. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-12-04T22:34:46Z 2023-09-21 2023-09-21T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/160874 |
| url |
http://hdl.handle.net/10362/160874 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2296-2646 PURE: 77680214 https://doi.org/10.3389/fchem.2023.1267563 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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14 application/pdf |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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