A scalable method to purify reflectins from inclusion bodies

Detalhes bibliográficos
Autor(a) principal: Lychko, Iana
Data de Publicação: 2023
Outros Autores: Soares, Cátia Lopes, Dias, Ana Margarida Gonçalves Carvalho, Roque, Ana Cecília Afonso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/154776
Resumo: Associate Laboratory Institute for Health and Bioeconomy – i4HB (LA/P/0140/2020). 2022.11305.BD for C.S. Publisher Copyright: © 2023 The Author(s)
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spelling A scalable method to purify reflectins from inclusion bodiesBio-based materialsInclusion bodiesPurificationReflectinsSelf-assemblyStructural proteinsAnalytical ChemistryFiltration and SeparationAssociate Laboratory Institute for Health and Bioeconomy – i4HB (LA/P/0140/2020). 2022.11305.BD for C.S. Publisher Copyright: © 2023 The Author(s)Structural proteins are an attractive inspiration for functional biobased materials. In nature, cephalopods skin colour modulation is related to the dynamic self-assembly of a family of structural proteins known as reflectins. To fully reach their potential as engineered bio-based materials, reflectins need to be produced by biotechnological means. One of the challenges is associated with establishing and optimizing reflectin purification processes to achieve the highest yield and productivity. Here, we studied purification strategies for two reflectin sequences from different organisms which were recombinantly expressed in a bacterial host at laboratory scale. Reflectins purification was then assessed by two chromatographic and one non-chromatographic methods. Methods were compared considering final purity and yield, productivity, cost and sustainability. The non-chromatographic method based on inclusion bodies washing presented the most promising results (protein purity > 90% and purification yields up to 88%). Our results contribute to define bioprocessing strategies to address the vision of biodegradable and sustainable protein-based materials.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNLychko, IanaSoares, Cátia LopesDias, Ana Margarida Gonçalves CarvalhoRoque, Ana Cecília Afonso2023-07-03T22:17:41Z2023-06-152023-06-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/154776eng1383-5866PURE: 65212928https://doi.org/10.1016/j.seppur.2023.123736info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:37:13Zoai:run.unl.pt:10362/154776Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:45.052953Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A scalable method to purify reflectins from inclusion bodies
title A scalable method to purify reflectins from inclusion bodies
spellingShingle A scalable method to purify reflectins from inclusion bodies
Lychko, Iana
Bio-based materials
Inclusion bodies
Purification
Reflectins
Self-assembly
Structural proteins
Analytical Chemistry
Filtration and Separation
title_short A scalable method to purify reflectins from inclusion bodies
title_full A scalable method to purify reflectins from inclusion bodies
title_fullStr A scalable method to purify reflectins from inclusion bodies
title_full_unstemmed A scalable method to purify reflectins from inclusion bodies
title_sort A scalable method to purify reflectins from inclusion bodies
author Lychko, Iana
author_facet Lychko, Iana
Soares, Cátia Lopes
Dias, Ana Margarida Gonçalves Carvalho
Roque, Ana Cecília Afonso
author_role author
author2 Soares, Cátia Lopes
Dias, Ana Margarida Gonçalves Carvalho
Roque, Ana Cecília Afonso
author2_role author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Lychko, Iana
Soares, Cátia Lopes
Dias, Ana Margarida Gonçalves Carvalho
Roque, Ana Cecília Afonso
dc.subject.por.fl_str_mv Bio-based materials
Inclusion bodies
Purification
Reflectins
Self-assembly
Structural proteins
Analytical Chemistry
Filtration and Separation
topic Bio-based materials
Inclusion bodies
Purification
Reflectins
Self-assembly
Structural proteins
Analytical Chemistry
Filtration and Separation
description Associate Laboratory Institute for Health and Bioeconomy – i4HB (LA/P/0140/2020). 2022.11305.BD for C.S. Publisher Copyright: © 2023 The Author(s)
publishDate 2023
dc.date.none.fl_str_mv 2023-07-03T22:17:41Z
2023-06-15
2023-06-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/154776
url http://hdl.handle.net/10362/154776
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1383-5866
PURE: 65212928
https://doi.org/10.1016/j.seppur.2023.123736
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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