Control of uterine microenvironment by foxp3(+) cells facilitates embryo implantation
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/109741 https://doi.org/10.3389/fimmu.2013.00158 |
Resumo: | Implantation of the fertilized egg into the maternal uterus depends on the fine balance between inflammatory and anti-inflammatory processes. Whilst regulatory T cells (Tregs) are reportedly involved in protection of allogeneic fetuses against rejection by the maternal immune system, their role for pregnancy to establish, e.g., blastocyst implantation, is not clear. By using 2-photon imaging we show that Foxp3(+) cells accumulated in the mouse uterus during the receptive phase of the estrus cycle. Seminal fluid further fostered Treg expansion. Depletion of Tregs in two Foxp3.DTR-based models prior to pairing drastically impaired implantation and resulted in infiltration of activated T effector cells as well as in uterine inflammation and fibrosis in both allogeneic and syngeneic mating combinations. Genetic deletion of the homing receptor CCR7 interfered with accumulation of Tregs in the uterus and implantation indicating that homing of Tregs to the uterus was mediated by CCR7. Our results demonstrate that Tregs play a critical role in embryo implantation by preventing the development of a hostile uterine microenvironment. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Control of uterine microenvironment by foxp3(+) cells facilitates embryo implantationregulatoryT cellsimplantationpregnancyfibrosisinflammationImplantation of the fertilized egg into the maternal uterus depends on the fine balance between inflammatory and anti-inflammatory processes. Whilst regulatory T cells (Tregs) are reportedly involved in protection of allogeneic fetuses against rejection by the maternal immune system, their role for pregnancy to establish, e.g., blastocyst implantation, is not clear. By using 2-photon imaging we show that Foxp3(+) cells accumulated in the mouse uterus during the receptive phase of the estrus cycle. Seminal fluid further fostered Treg expansion. Depletion of Tregs in two Foxp3.DTR-based models prior to pairing drastically impaired implantation and resulted in infiltration of activated T effector cells as well as in uterine inflammation and fibrosis in both allogeneic and syngeneic mating combinations. Genetic deletion of the homing receptor CCR7 interfered with accumulation of Tregs in the uterus and implantation indicating that homing of Tregs to the uterus was mediated by CCR7. Our results demonstrate that Tregs play a critical role in embryo implantation by preventing the development of a hostile uterine microenvironment.Frontiers Media S.A.2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/109741http://hdl.handle.net/10316/109741https://doi.org/10.3389/fimmu.2013.00158eng1664-3224Teles, AnaSchumacher, AnnKühnle, Marie-CristineLinzke, NadjaThuere, CatharinaReichardt, PeterTadokoro, Carlos EduardoHämmerling, Günter JZenclussen, Ana Claudiainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-24T13:23:05Zoai:estudogeral.uc.pt:10316/109741Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:53.576684Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Control of uterine microenvironment by foxp3(+) cells facilitates embryo implantation |
title |
Control of uterine microenvironment by foxp3(+) cells facilitates embryo implantation |
spellingShingle |
Control of uterine microenvironment by foxp3(+) cells facilitates embryo implantation Teles, Ana regulatoryT cells implantation pregnancy fibrosis inflammation |
title_short |
Control of uterine microenvironment by foxp3(+) cells facilitates embryo implantation |
title_full |
Control of uterine microenvironment by foxp3(+) cells facilitates embryo implantation |
title_fullStr |
Control of uterine microenvironment by foxp3(+) cells facilitates embryo implantation |
title_full_unstemmed |
Control of uterine microenvironment by foxp3(+) cells facilitates embryo implantation |
title_sort |
Control of uterine microenvironment by foxp3(+) cells facilitates embryo implantation |
author |
Teles, Ana |
author_facet |
Teles, Ana Schumacher, Ann Kühnle, Marie-Cristine Linzke, Nadja Thuere, Catharina Reichardt, Peter Tadokoro, Carlos Eduardo Hämmerling, Günter J Zenclussen, Ana Claudia |
author_role |
author |
author2 |
Schumacher, Ann Kühnle, Marie-Cristine Linzke, Nadja Thuere, Catharina Reichardt, Peter Tadokoro, Carlos Eduardo Hämmerling, Günter J Zenclussen, Ana Claudia |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Teles, Ana Schumacher, Ann Kühnle, Marie-Cristine Linzke, Nadja Thuere, Catharina Reichardt, Peter Tadokoro, Carlos Eduardo Hämmerling, Günter J Zenclussen, Ana Claudia |
dc.subject.por.fl_str_mv |
regulatoryT cells implantation pregnancy fibrosis inflammation |
topic |
regulatoryT cells implantation pregnancy fibrosis inflammation |
description |
Implantation of the fertilized egg into the maternal uterus depends on the fine balance between inflammatory and anti-inflammatory processes. Whilst regulatory T cells (Tregs) are reportedly involved in protection of allogeneic fetuses against rejection by the maternal immune system, their role for pregnancy to establish, e.g., blastocyst implantation, is not clear. By using 2-photon imaging we show that Foxp3(+) cells accumulated in the mouse uterus during the receptive phase of the estrus cycle. Seminal fluid further fostered Treg expansion. Depletion of Tregs in two Foxp3.DTR-based models prior to pairing drastically impaired implantation and resulted in infiltration of activated T effector cells as well as in uterine inflammation and fibrosis in both allogeneic and syngeneic mating combinations. Genetic deletion of the homing receptor CCR7 interfered with accumulation of Tregs in the uterus and implantation indicating that homing of Tregs to the uterus was mediated by CCR7. Our results demonstrate that Tregs play a critical role in embryo implantation by preventing the development of a hostile uterine microenvironment. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/109741 http://hdl.handle.net/10316/109741 https://doi.org/10.3389/fimmu.2013.00158 |
url |
http://hdl.handle.net/10316/109741 https://doi.org/10.3389/fimmu.2013.00158 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1664-3224 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Frontiers Media S.A. |
publisher.none.fl_str_mv |
Frontiers Media S.A. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134140798337024 |