Membrane progesterone receptors in human regulatory T cells: a reality in pregnancy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/28266 https://doi.org/10.1111/1471-0528.13294 |
Resumo: | Objective - To provide evidence of the existence of membrane progesterone receptor alpha (mPRa) on regulatory T cells (Treg) in peripheral blood during pregnancy, postulating a possible explanation for the effect of progesterone on preterm birth. Design Cross-sectional study. Setting - Tertiary Obstetric Department in a University Hospital. Population - Healthy pregnant women. Methods - Treg cells from peripheral blood samples were studied by flow cytometry using multiple monoclonal antibody expression. Main outcome measures - Evaluate the number and percentage of CD4+CD25highCD127low, the number and percentage of Treg cells among the total CD4+ T cells, and the percentage and mean fluorescence intensity (MFI) of mPRa in that population, using several gating strategies. Results - 43 peripheral blood samples were collected from healthy women during pregnancy, whose median gestational age was 28.7 7.1 (16–40) weeks. The percentage of CD4+ in the total lymphocytes was 43% (32–51) and the percentage of CD4+CD25highCD127low was 4.8% (1.6–5.9), with only 45% (16–72) of those cells expressing the intracellular marker FoxP3 (Treg cell pool). We confirmed the existence of mPRa in that specific population because 8.0% (2.02–33) of the Treg cells were marked with the specific monoclonal antibody, with an mPRa+ MFI of 719 (590–1471). Conclusions - This research shows that Treg cells express mPRa during pregnancy, which might play an important role in immune modulation by progesterone. |
id |
RCAP_b53b4d8d5a2897db8c15901cbaca6385 |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/28266 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Membrane progesterone receptors in human regulatory T cells: a reality in pregnancyPregnancyProgesterone receptorT regulatory cellsObjective - To provide evidence of the existence of membrane progesterone receptor alpha (mPRa) on regulatory T cells (Treg) in peripheral blood during pregnancy, postulating a possible explanation for the effect of progesterone on preterm birth. Design Cross-sectional study. Setting - Tertiary Obstetric Department in a University Hospital. Population - Healthy pregnant women. Methods - Treg cells from peripheral blood samples were studied by flow cytometry using multiple monoclonal antibody expression. Main outcome measures - Evaluate the number and percentage of CD4+CD25highCD127low, the number and percentage of Treg cells among the total CD4+ T cells, and the percentage and mean fluorescence intensity (MFI) of mPRa in that population, using several gating strategies. Results - 43 peripheral blood samples were collected from healthy women during pregnancy, whose median gestational age was 28.7 7.1 (16–40) weeks. The percentage of CD4+ in the total lymphocytes was 43% (32–51) and the percentage of CD4+CD25highCD127low was 4.8% (1.6–5.9), with only 45% (16–72) of those cells expressing the intracellular marker FoxP3 (Treg cell pool). We confirmed the existence of mPRa in that specific population because 8.0% (2.02–33) of the Treg cells were marked with the specific monoclonal antibody, with an mPRa+ MFI of 719 (590–1471). Conclusions - This research shows that Treg cells express mPRa during pregnancy, which might play an important role in immune modulation by progesterone.Royal College of Obstetricians and Gynaecologists2015-02-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/28266http://hdl.handle.net/10316/28266https://doi.org/10.1111/1471-0528.13294enghttp://onlinelibrary.wiley.com/doi/10.1111/1471-0528.13294/abstractAreia, AnaVale-Pereira, SofiaAlves, VeraRodrigues-Santos, PauloMoura, PauloMota-Pinto, Anabelainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-01-14T11:49:27Zoai:estudogeral.uc.pt:10316/28266Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:43:25.071371Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Membrane progesterone receptors in human regulatory T cells: a reality in pregnancy |
title |
Membrane progesterone receptors in human regulatory T cells: a reality in pregnancy |
spellingShingle |
Membrane progesterone receptors in human regulatory T cells: a reality in pregnancy Areia, Ana Pregnancy Progesterone receptor T regulatory cells |
title_short |
Membrane progesterone receptors in human regulatory T cells: a reality in pregnancy |
title_full |
Membrane progesterone receptors in human regulatory T cells: a reality in pregnancy |
title_fullStr |
Membrane progesterone receptors in human regulatory T cells: a reality in pregnancy |
title_full_unstemmed |
Membrane progesterone receptors in human regulatory T cells: a reality in pregnancy |
title_sort |
Membrane progesterone receptors in human regulatory T cells: a reality in pregnancy |
author |
Areia, Ana |
author_facet |
Areia, Ana Vale-Pereira, Sofia Alves, Vera Rodrigues-Santos, Paulo Moura, Paulo Mota-Pinto, Anabela |
author_role |
author |
author2 |
Vale-Pereira, Sofia Alves, Vera Rodrigues-Santos, Paulo Moura, Paulo Mota-Pinto, Anabela |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Areia, Ana Vale-Pereira, Sofia Alves, Vera Rodrigues-Santos, Paulo Moura, Paulo Mota-Pinto, Anabela |
dc.subject.por.fl_str_mv |
Pregnancy Progesterone receptor T regulatory cells |
topic |
Pregnancy Progesterone receptor T regulatory cells |
description |
Objective - To provide evidence of the existence of membrane progesterone receptor alpha (mPRa) on regulatory T cells (Treg) in peripheral blood during pregnancy, postulating a possible explanation for the effect of progesterone on preterm birth. Design Cross-sectional study. Setting - Tertiary Obstetric Department in a University Hospital. Population - Healthy pregnant women. Methods - Treg cells from peripheral blood samples were studied by flow cytometry using multiple monoclonal antibody expression. Main outcome measures - Evaluate the number and percentage of CD4+CD25highCD127low, the number and percentage of Treg cells among the total CD4+ T cells, and the percentage and mean fluorescence intensity (MFI) of mPRa in that population, using several gating strategies. Results - 43 peripheral blood samples were collected from healthy women during pregnancy, whose median gestational age was 28.7 7.1 (16–40) weeks. The percentage of CD4+ in the total lymphocytes was 43% (32–51) and the percentage of CD4+CD25highCD127low was 4.8% (1.6–5.9), with only 45% (16–72) of those cells expressing the intracellular marker FoxP3 (Treg cell pool). We confirmed the existence of mPRa in that specific population because 8.0% (2.02–33) of the Treg cells were marked with the specific monoclonal antibody, with an mPRa+ MFI of 719 (590–1471). Conclusions - This research shows that Treg cells express mPRa during pregnancy, which might play an important role in immune modulation by progesterone. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/28266 http://hdl.handle.net/10316/28266 https://doi.org/10.1111/1471-0528.13294 |
url |
http://hdl.handle.net/10316/28266 https://doi.org/10.1111/1471-0528.13294 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://onlinelibrary.wiley.com/doi/10.1111/1471-0528.13294/abstract |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Royal College of Obstetricians and Gynaecologists |
publisher.none.fl_str_mv |
Royal College of Obstetricians and Gynaecologists |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133706558898176 |