Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5053 https://doi.org/10.1016/j.talanta.2006.08.032 |
Resumo: | A flow cell has been designed for use with an electrochemical enzyme biosensor, based on low-cost carbon-film electrodes. Three types of mediators were used: cobalt and copper hexacyanoferrates and poly(neutral red) (PNR), covered with glucose oxidase (GOx) immobilised by cross-linking with glutaraldehyde in the presence of bovine serum albumin or inside a oxysilane sol-gel network. Mixtures of sol-gel precursors were made from 3-aminopropyl-triethoxysilane (APTOS) together with methyltrimethoxysilane (MTMOS), methyltriethoxysilane (MTEOS), tetraethyloxysilane (TEOS) or 3-glycidoxypropyl-trimethoxysilane (GOPMOS), and the best chosen for encapsulation. Optimisation in batch mode, using amperometric detection at fixed potential, showed the PNR-GOx modified carbon-film electrodes to be best for flow analysis for both glutaraldehyde and sol-gel enzyme immobilisation. Both types of enzyme electrode were tested under flow conditions and the reproducibility and stability of the biosensors were evaluated. The biosensors were used for fermentation monitoring of glucose in grape must and interference studies were also performed. |
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Design and application of a flow cell for carbon-film based electrochemical enzyme biosensorsFlow cellElectrochemical enzyme biosensorEnzyme immobilisationSol-gelGlucose determinationGrape mustA flow cell has been designed for use with an electrochemical enzyme biosensor, based on low-cost carbon-film electrodes. Three types of mediators were used: cobalt and copper hexacyanoferrates and poly(neutral red) (PNR), covered with glucose oxidase (GOx) immobilised by cross-linking with glutaraldehyde in the presence of bovine serum albumin or inside a oxysilane sol-gel network. Mixtures of sol-gel precursors were made from 3-aminopropyl-triethoxysilane (APTOS) together with methyltrimethoxysilane (MTMOS), methyltriethoxysilane (MTEOS), tetraethyloxysilane (TEOS) or 3-glycidoxypropyl-trimethoxysilane (GOPMOS), and the best chosen for encapsulation. Optimisation in batch mode, using amperometric detection at fixed potential, showed the PNR-GOx modified carbon-film electrodes to be best for flow analysis for both glutaraldehyde and sol-gel enzyme immobilisation. Both types of enzyme electrode were tested under flow conditions and the reproducibility and stability of the biosensors were evaluated. The biosensors were used for fermentation monitoring of glucose in grape must and interference studies were also performed.http://www.sciencedirect.com/science/article/B6THP-4M0BH9Y-2/1/306f5db86217ea276bf808fb05c028862007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5053http://hdl.handle.net/10316/5053https://doi.org/10.1016/j.talanta.2006.08.032engTalanta. 71:5 (2007) 1893-1900Barsan, Madalina M.Klincar, JanjaBatic, MartinBrett, Christopher M. A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-07-27T09:46:37Zoai:estudogeral.uc.pt:10316/5053Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:16.596083Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors |
title |
Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors |
spellingShingle |
Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors Barsan, Madalina M. Flow cell Electrochemical enzyme biosensor Enzyme immobilisation Sol-gel Glucose determination Grape must |
title_short |
Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors |
title_full |
Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors |
title_fullStr |
Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors |
title_full_unstemmed |
Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors |
title_sort |
Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors |
author |
Barsan, Madalina M. |
author_facet |
Barsan, Madalina M. Klincar, Janja Batic, Martin Brett, Christopher M. A. |
author_role |
author |
author2 |
Klincar, Janja Batic, Martin Brett, Christopher M. A. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Barsan, Madalina M. Klincar, Janja Batic, Martin Brett, Christopher M. A. |
dc.subject.por.fl_str_mv |
Flow cell Electrochemical enzyme biosensor Enzyme immobilisation Sol-gel Glucose determination Grape must |
topic |
Flow cell Electrochemical enzyme biosensor Enzyme immobilisation Sol-gel Glucose determination Grape must |
description |
A flow cell has been designed for use with an electrochemical enzyme biosensor, based on low-cost carbon-film electrodes. Three types of mediators were used: cobalt and copper hexacyanoferrates and poly(neutral red) (PNR), covered with glucose oxidase (GOx) immobilised by cross-linking with glutaraldehyde in the presence of bovine serum albumin or inside a oxysilane sol-gel network. Mixtures of sol-gel precursors were made from 3-aminopropyl-triethoxysilane (APTOS) together with methyltrimethoxysilane (MTMOS), methyltriethoxysilane (MTEOS), tetraethyloxysilane (TEOS) or 3-glycidoxypropyl-trimethoxysilane (GOPMOS), and the best chosen for encapsulation. Optimisation in batch mode, using amperometric detection at fixed potential, showed the PNR-GOx modified carbon-film electrodes to be best for flow analysis for both glutaraldehyde and sol-gel enzyme immobilisation. Both types of enzyme electrode were tested under flow conditions and the reproducibility and stability of the biosensors were evaluated. The biosensors were used for fermentation monitoring of glucose in grape must and interference studies were also performed. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5053 http://hdl.handle.net/10316/5053 https://doi.org/10.1016/j.talanta.2006.08.032 |
url |
http://hdl.handle.net/10316/5053 https://doi.org/10.1016/j.talanta.2006.08.032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Talanta. 71:5 (2007) 1893-1900 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133904836231168 |