Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning

Detalhes bibliográficos
Autor(a) principal: Bayindir, Irem
Data de Publicação: 2015
Outros Autores: Babaeikelishomi, Rohollah, Kocanova, Silvia, Sousa, Isabel Sofia, Lerch, Sarah, Hardt, Olaf, Wild, Stefan, Bosio, Andreas, Bystricky, Kerstin, Herzig, Stephan, Vegiopoulos, Alexandros
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/109148
https://doi.org/10.3389/fendo.2015.00129
Resumo: De novo formation of beige/brite adipocytes from progenitor cells contributes to the thermogenic adaptation of adipose tissue and holds great potential for the therapeutic remodeling of fat as a treatment for obesity. Despite the recent identification of several factors regulating browning of white fat, there is a lack of physiological cell models for the mechanistic investigation of progenitor-mediated beige/brite differentiation. We have previously revealed prostacyclin (PGI2) as one of the few known endogenous extracellular mediators promoting de novo beige/brite formation by relaying β-adrenergic stimulation to the progenitor level. Here, we present a cell model based on murine primary progenitor cells defined by markers previously shown to be relevant for in vivo browning, including a simplified isolation procedure. We demonstrate the specific and broad induction of thermogenic gene expression by PGI2 signaling in the absence of lineage conversion, and reveal the previously unidentified nuclear relocalization of the Ucp1 gene locus in association with transcriptional activation. By profiling the time course of the progenitor response, we show that PGI2 signaling promoted progenitor cell activation through cell cycle and adhesion pathways prior to metabolic maturation toward an oxidative cell phenotype. Our results highlight the importance of core progenitor activation pathways for the recruitment of thermogenic cells and provide a resource for further mechanistic investigation.
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spelling Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browningbeige/brite differentiationadipocyte progenitorsprostacyclinPGI2adipocyte cell modeladipose tissue remodelingnuclear localizationDe novo formation of beige/brite adipocytes from progenitor cells contributes to the thermogenic adaptation of adipose tissue and holds great potential for the therapeutic remodeling of fat as a treatment for obesity. Despite the recent identification of several factors regulating browning of white fat, there is a lack of physiological cell models for the mechanistic investigation of progenitor-mediated beige/brite differentiation. We have previously revealed prostacyclin (PGI2) as one of the few known endogenous extracellular mediators promoting de novo beige/brite formation by relaying β-adrenergic stimulation to the progenitor level. Here, we present a cell model based on murine primary progenitor cells defined by markers previously shown to be relevant for in vivo browning, including a simplified isolation procedure. We demonstrate the specific and broad induction of thermogenic gene expression by PGI2 signaling in the absence of lineage conversion, and reveal the previously unidentified nuclear relocalization of the Ucp1 gene locus in association with transcriptional activation. By profiling the time course of the progenitor response, we show that PGI2 signaling promoted progenitor cell activation through cell cycle and adhesion pathways prior to metabolic maturation toward an oxidative cell phenotype. Our results highlight the importance of core progenitor activation pathways for the recruitment of thermogenic cells and provide a resource for further mechanistic investigation.Frontiers Media S.A.2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/109148http://hdl.handle.net/10316/109148https://doi.org/10.3389/fendo.2015.00129eng1664-2392Bayindir, IremBabaeikelishomi, RohollahKocanova, SilviaSousa, Isabel SofiaLerch, SarahHardt, OlafWild, StefanBosio, AndreasBystricky, KerstinHerzig, StephanVegiopoulos, Alexandrosinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-29T08:08:37Zoai:estudogeral.uc.pt:10316/109148Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:20.806774Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning
title Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning
spellingShingle Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning
Bayindir, Irem
beige/brite differentiation
adipocyte progenitors
prostacyclin
PGI2
adipocyte cell model
adipose tissue remodeling
nuclear localization
title_short Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning
title_full Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning
title_fullStr Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning
title_full_unstemmed Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning
title_sort Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning
author Bayindir, Irem
author_facet Bayindir, Irem
Babaeikelishomi, Rohollah
Kocanova, Silvia
Sousa, Isabel Sofia
Lerch, Sarah
Hardt, Olaf
Wild, Stefan
Bosio, Andreas
Bystricky, Kerstin
Herzig, Stephan
Vegiopoulos, Alexandros
author_role author
author2 Babaeikelishomi, Rohollah
Kocanova, Silvia
Sousa, Isabel Sofia
Lerch, Sarah
Hardt, Olaf
Wild, Stefan
Bosio, Andreas
Bystricky, Kerstin
Herzig, Stephan
Vegiopoulos, Alexandros
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bayindir, Irem
Babaeikelishomi, Rohollah
Kocanova, Silvia
Sousa, Isabel Sofia
Lerch, Sarah
Hardt, Olaf
Wild, Stefan
Bosio, Andreas
Bystricky, Kerstin
Herzig, Stephan
Vegiopoulos, Alexandros
dc.subject.por.fl_str_mv beige/brite differentiation
adipocyte progenitors
prostacyclin
PGI2
adipocyte cell model
adipose tissue remodeling
nuclear localization
topic beige/brite differentiation
adipocyte progenitors
prostacyclin
PGI2
adipocyte cell model
adipose tissue remodeling
nuclear localization
description De novo formation of beige/brite adipocytes from progenitor cells contributes to the thermogenic adaptation of adipose tissue and holds great potential for the therapeutic remodeling of fat as a treatment for obesity. Despite the recent identification of several factors regulating browning of white fat, there is a lack of physiological cell models for the mechanistic investigation of progenitor-mediated beige/brite differentiation. We have previously revealed prostacyclin (PGI2) as one of the few known endogenous extracellular mediators promoting de novo beige/brite formation by relaying β-adrenergic stimulation to the progenitor level. Here, we present a cell model based on murine primary progenitor cells defined by markers previously shown to be relevant for in vivo browning, including a simplified isolation procedure. We demonstrate the specific and broad induction of thermogenic gene expression by PGI2 signaling in the absence of lineage conversion, and reveal the previously unidentified nuclear relocalization of the Ucp1 gene locus in association with transcriptional activation. By profiling the time course of the progenitor response, we show that PGI2 signaling promoted progenitor cell activation through cell cycle and adhesion pathways prior to metabolic maturation toward an oxidative cell phenotype. Our results highlight the importance of core progenitor activation pathways for the recruitment of thermogenic cells and provide a resource for further mechanistic investigation.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/109148
http://hdl.handle.net/10316/109148
https://doi.org/10.3389/fendo.2015.00129
url http://hdl.handle.net/10316/109148
https://doi.org/10.3389/fendo.2015.00129
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-2392
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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