Innovative on-line SPEHPLC-FD method for the analysis of Bisphenol A in saliva

• Autor(a) principal: Prost-Tournier, Oséa Marie Poe Rava
• Data de Publicação: 2024
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/20.500.11816/4698
• topic: BISPHENOL A (BPA); SPE-HPLC-FD; SALIVA; RESIN-MATRIX; DENTAL COMPOSITE

Introduction: Bisphenol A (BPA) is a synthetic organic compound found in the organic matrix of composite resins used in dentistry. It represents a potential risk for patients requiring composite resin treatments, as BPA is recognised as an endocrine disruptor. Objective: To develop and validate an effective on-line SPE-LC-FD method for quantifying BPA in saliva and apply it to real samples. Materials and Methods: Before on-line SPE-HPLC-FD analysis, 6 mL of acetonitrile was added to a 2 mL aliquot of saliva to precipitate the proteins. The supernatant was then evaporated and reconstituted in a 2 mL solution of 5% ethanol. A 500 L aliquot was injected directly into the HPLC system. The initial step involved cleaning the sample on the on-line SPE column (Restricted Access Media column; RP-18 ADS (20 mm × 4 mm; 25 m)), after which the retained components were automatically transferred to the analytical column (Luna PFP column (2) (150 mm × 4.6 mm; 3 m, 100 Å)) for component resolution and subsequent fluorescence detection. Results: The method, validated according to the ICH Guidelines, revealed to be accurate and precise under the linear range, with detection and quantification limits of 1 ng/mL and 5 ng/mL, respectively. Discussion: The detection of BPA in saliva after a dental treatment requires a more sensitive method with low quantification limits. Conclusion: The on-line SPE-HPLC-FD method that was developed is efficient and suitable for quantifying BPA in· saliva.