Chicken antibodies are highly suitable for particle enhanced turbidimetric assays

Detalhes bibliográficos
Autor(a) principal: Larsson, Anders
Data de Publicação: 2022
Outros Autores: Campbell, Andrew, Eriksson, Mats
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/145220
Resumo: Funding Information: The project was supported by funding from the Uppsala University Research Fund. The kappa free light chain study was supported by Eurostars (grant number 114334). Publisher Copyright: Copyright © 2022 Larsson, Campbell and Eriksson.
id RCAP_b416c4050c2b8a9d85293ed900e5d19d
oai_identifier_str oai:run.unl.pt:10362/145220
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Chicken antibodies are highly suitable for particle enhanced turbidimetric assayschicken IgYcystatin Cfree light chainsimmunoassaysparticle enhanced turbidimetric assayPETIAImmunology and AllergyImmunologyFunding Information: The project was supported by funding from the Uppsala University Research Fund. The kappa free light chain study was supported by Eurostars (grant number 114334). Publisher Copyright: Copyright © 2022 Larsson, Campbell and Eriksson.Antibody-based assays are commonly used in clinical laboratories for analyzing plasma, serum and other samples for particular protein markers. Although such assays have been traditionally based on antibodies raised in mammals (e.g., mice, rabbits, goats), there are several advantages of using avian antibodies (IgY) raised in chickens, including production volumes, costs, and ethical/animal welfare considerations. A further disadvantage of using mammalian IgG in such assays is the potential for agglutination when exposed to rheumatoid factor (RF) in serum. However, when used in the free form the immune complexes formed with avian antibodies have been reported to have less ability than those formed with mammalian antibodies to cause the light scatter which are used for instrument measurement. In addition, when the amount of antigen exceeds the maximum precipitating point in relation to the amount of antibody, there is a rapid decline in the absorbance values of the immune complexes (antigen excess) when IgY is used. However, when avian antibodies are conjugated to a substrate and used in particle enhanced turbidimetric assays (PETIA), these problems are avoided. Here we investigated three clinical assays using chicken antibodies, one using free (unbound) IgY and two with IgY-based PETIA. The IgY PETIA demonstrated a strong scatter response, even at high antigen concentrations in contrast to the steep decline seen with free IgY antibodies. IgY PETIA reagents can provide test results with low coefficient of variation (<1% for duplicate samples). We also investigated the effect of RF on agglutination of mammalian antibodies (IgG from mouse, rabbit, sheep, and human) and chicken antibodies. Whereas agglutination was observed with all the mammalian antibodies in the presence of RF, this was not observed at all with chicken IgY. Our results support the growing body of evidence that chicken egg yolks can thus be a valuable source of antibodies for use in PETIA in clinical laboratories.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNLarsson, AndersCampbell, AndrewEriksson, Mats2022-11-03T22:14:05Z2022-10-112022-10-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/145220eng1664-3224PURE: 47542880https://doi.org/10.3389/fimmu.2022.1016781info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:25:24Zoai:run.unl.pt:10362/145220Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:58.144486Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chicken antibodies are highly suitable for particle enhanced turbidimetric assays
title Chicken antibodies are highly suitable for particle enhanced turbidimetric assays
spellingShingle Chicken antibodies are highly suitable for particle enhanced turbidimetric assays
Larsson, Anders
chicken IgY
cystatin C
free light chains
immunoassays
particle enhanced turbidimetric assay
PETIA
Immunology and Allergy
Immunology
title_short Chicken antibodies are highly suitable for particle enhanced turbidimetric assays
title_full Chicken antibodies are highly suitable for particle enhanced turbidimetric assays
title_fullStr Chicken antibodies are highly suitable for particle enhanced turbidimetric assays
title_full_unstemmed Chicken antibodies are highly suitable for particle enhanced turbidimetric assays
title_sort Chicken antibodies are highly suitable for particle enhanced turbidimetric assays
author Larsson, Anders
author_facet Larsson, Anders
Campbell, Andrew
Eriksson, Mats
author_role author
author2 Campbell, Andrew
Eriksson, Mats
author2_role author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Larsson, Anders
Campbell, Andrew
Eriksson, Mats
dc.subject.por.fl_str_mv chicken IgY
cystatin C
free light chains
immunoassays
particle enhanced turbidimetric assay
PETIA
Immunology and Allergy
Immunology
topic chicken IgY
cystatin C
free light chains
immunoassays
particle enhanced turbidimetric assay
PETIA
Immunology and Allergy
Immunology
description Funding Information: The project was supported by funding from the Uppsala University Research Fund. The kappa free light chain study was supported by Eurostars (grant number 114334). Publisher Copyright: Copyright © 2022 Larsson, Campbell and Eriksson.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-03T22:14:05Z
2022-10-11
2022-10-11T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/145220
url http://hdl.handle.net/10362/145220
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-3224
PURE: 47542880
https://doi.org/10.3389/fimmu.2022.1016781
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138111765086208

Registros relacionados