Studies on the impact of selected pretreatments on protein solubility of Arthrospira platensis microalga

Detalhes bibliográficos
Autor(a) principal: Spínola, Maria P.
Data de Publicação: 2023
Outros Autores: Costa, Mónica M., Prates, José A.M
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/27788
Resumo: Research Areas: Agriculture
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spelling Studies on the impact of selected pretreatments on protein solubility of Arthrospira platensis microalgaArthrospira sp.microalgaemechanical pretreatmentsproteins solubilityResearch Areas: AgricultureArthrospira platensis has emerged as a novel protein feed source since it contains high protein level and quality. However, this microalga presents a recalcitrant cell wall and its main proteins form protein-pigment complexes attached to the thylakoid membrane. The objective of the present study was to evaluate the influence of mechanical/physical pretreatments (bead milling, extrusion, freeze-drying, heating, microwave and sonication) on A. platensis protein solubility. Total protein content and solubility were assessed by Bradford method and SDS-PAGE quantification. Protein degradation was assessed through quantification of protein fractions (18–26 kDa, 40–48 kDa and others) in SDS-PAGE gels. Peptide formation was evaluated using the o-phthaldialdehyde assay. The results showed a decrease in total protein content in the supernatant with extrusion (0.07 to 1.42 mg/mL) and microwave pretreatments, and in the pellet with extrusion. Therefore, extrusion, followed by microwave, was the most effective pretreatment for A. platensis proteins denaturation and solubility. It is suggested that the extrusion process cause an irreversible denaturation and aggregation of the major microalga proteins (c-phycocyanin and allophycocyanin), with a strong decrease in their solubility. Therefore, extrusion could increase the bioaccessibility of A. platensis proteins and enable the incorporation of this microalga at higher levels in monogastric dietsMDPIRepositório da Universidade de LisboaSpínola, Maria P.Costa, Mónica M.Prates, José A.M2023-05-21T18:11:31Z2023-01-162023-01-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/27788engSpínola MP, Costa MM, Prates JAM. 2023. Studies on the Impact of Selected Pretreatments on Protein Solubility of Arthrospira platensis Microalga. Agriculture, 13(1):221. DOI: https://doi.org/10.3390/agriculture1301022110.3390/agriculture130102212077-0472info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-05-28T01:31:10Zoai:www.repository.utl.pt:10400.5/27788Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:56:28.344069Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Studies on the impact of selected pretreatments on protein solubility of Arthrospira platensis microalga
title Studies on the impact of selected pretreatments on protein solubility of Arthrospira platensis microalga
spellingShingle Studies on the impact of selected pretreatments on protein solubility of Arthrospira platensis microalga
Spínola, Maria P.
Arthrospira sp.
microalgae
mechanical pretreatments
proteins solubility
title_short Studies on the impact of selected pretreatments on protein solubility of Arthrospira platensis microalga
title_full Studies on the impact of selected pretreatments on protein solubility of Arthrospira platensis microalga
title_fullStr Studies on the impact of selected pretreatments on protein solubility of Arthrospira platensis microalga
title_full_unstemmed Studies on the impact of selected pretreatments on protein solubility of Arthrospira platensis microalga
title_sort Studies on the impact of selected pretreatments on protein solubility of Arthrospira platensis microalga
author Spínola, Maria P.
author_facet Spínola, Maria P.
Costa, Mónica M.
Prates, José A.M
author_role author
author2 Costa, Mónica M.
Prates, José A.M
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Spínola, Maria P.
Costa, Mónica M.
Prates, José A.M
dc.subject.por.fl_str_mv Arthrospira sp.
microalgae
mechanical pretreatments
proteins solubility
topic Arthrospira sp.
microalgae
mechanical pretreatments
proteins solubility
description Research Areas: Agriculture
publishDate 2023
dc.date.none.fl_str_mv 2023-05-21T18:11:31Z
2023-01-16
2023-01-16T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/27788
url http://hdl.handle.net/10400.5/27788
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Spínola MP, Costa MM, Prates JAM. 2023. Studies on the Impact of Selected Pretreatments on Protein Solubility of Arthrospira platensis Microalga. Agriculture, 13(1):221. DOI: https://doi.org/10.3390/agriculture13010221
10.3390/agriculture13010221
2077-0472
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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