Comparative Cr, As and CCA induced Cytostaticity in mice kidney: a contribution to assess CCA toxicity
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/36946 |
Resumo: | CCA (Chromium Copper Arsenate) treated wood, widely used in outdoor residential structures and playgrounds, poses considerable dangers of leaching of its components to the environment. In this study, mouse kidney samples were used to evaluate the effects of CCA, chromium trioxide (CrO3) and arsenic pentoxide (As2O5) on cell pathophysiology by flow cytometry. Samples were collected after 14, 24, 48 and 96 h of animal exposure. While Cr had no statistically significant cytostatic effects, As2O5 induced a S-phase delay in animals exposed for 24 h, and over time a G0/G1 phase blockage. The effects of CCA in S-phase were similar, but more severe than those of As2O5. Since environmental and public health hazards due to the long durability of CCA-treated wood products, these data confirm that CCA has profoundly toxic effects on cell cycle, distinct from the compounds themselves. These cytostatic effects support cell cycle dynamics as a valuable endpoint to assess the toxicity of remaining CCA-treated infrastructures, and the expected increased waste stream over the coming decades. |
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7160 |
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Comparative Cr, As and CCA induced Cytostaticity in mice kidney: a contribution to assess CCA toxicityCCAArseniteChromiumClastogenicityCytostaticityKidneyCCA (Chromium Copper Arsenate) treated wood, widely used in outdoor residential structures and playgrounds, poses considerable dangers of leaching of its components to the environment. In this study, mouse kidney samples were used to evaluate the effects of CCA, chromium trioxide (CrO3) and arsenic pentoxide (As2O5) on cell pathophysiology by flow cytometry. Samples were collected after 14, 24, 48 and 96 h of animal exposure. While Cr had no statistically significant cytostatic effects, As2O5 induced a S-phase delay in animals exposed for 24 h, and over time a G0/G1 phase blockage. The effects of CCA in S-phase were similar, but more severe than those of As2O5. Since environmental and public health hazards due to the long durability of CCA-treated wood products, these data confirm that CCA has profoundly toxic effects on cell cycle, distinct from the compounds themselves. These cytostatic effects support cell cycle dynamics as a valuable endpoint to assess the toxicity of remaining CCA-treated infrastructures, and the expected increased waste stream over the coming decades.Elsevier2023-04-12T14:03:27Z2020-01-01T00:00:00Z2020-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/36946eng1382-668910.1016/j.etap.2019.103297Matos, Rita CerejeiraOliveira, HelenaFonseca, Henrique M. A. C.Morais, SimoneSharma, BechanSantos, Conceiçãode Lourdes Pereira, Mariainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:11:16Zoai:ria.ua.pt:10773/36946Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:07:36.701815Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Comparative Cr, As and CCA induced Cytostaticity in mice kidney: a contribution to assess CCA toxicity |
title |
Comparative Cr, As and CCA induced Cytostaticity in mice kidney: a contribution to assess CCA toxicity |
spellingShingle |
Comparative Cr, As and CCA induced Cytostaticity in mice kidney: a contribution to assess CCA toxicity Matos, Rita Cerejeira CCA Arsenite Chromium Clastogenicity Cytostaticity Kidney |
title_short |
Comparative Cr, As and CCA induced Cytostaticity in mice kidney: a contribution to assess CCA toxicity |
title_full |
Comparative Cr, As and CCA induced Cytostaticity in mice kidney: a contribution to assess CCA toxicity |
title_fullStr |
Comparative Cr, As and CCA induced Cytostaticity in mice kidney: a contribution to assess CCA toxicity |
title_full_unstemmed |
Comparative Cr, As and CCA induced Cytostaticity in mice kidney: a contribution to assess CCA toxicity |
title_sort |
Comparative Cr, As and CCA induced Cytostaticity in mice kidney: a contribution to assess CCA toxicity |
author |
Matos, Rita Cerejeira |
author_facet |
Matos, Rita Cerejeira Oliveira, Helena Fonseca, Henrique M. A. C. Morais, Simone Sharma, Bechan Santos, Conceição de Lourdes Pereira, Maria |
author_role |
author |
author2 |
Oliveira, Helena Fonseca, Henrique M. A. C. Morais, Simone Sharma, Bechan Santos, Conceição de Lourdes Pereira, Maria |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Matos, Rita Cerejeira Oliveira, Helena Fonseca, Henrique M. A. C. Morais, Simone Sharma, Bechan Santos, Conceição de Lourdes Pereira, Maria |
dc.subject.por.fl_str_mv |
CCA Arsenite Chromium Clastogenicity Cytostaticity Kidney |
topic |
CCA Arsenite Chromium Clastogenicity Cytostaticity Kidney |
description |
CCA (Chromium Copper Arsenate) treated wood, widely used in outdoor residential structures and playgrounds, poses considerable dangers of leaching of its components to the environment. In this study, mouse kidney samples were used to evaluate the effects of CCA, chromium trioxide (CrO3) and arsenic pentoxide (As2O5) on cell pathophysiology by flow cytometry. Samples were collected after 14, 24, 48 and 96 h of animal exposure. While Cr had no statistically significant cytostatic effects, As2O5 induced a S-phase delay in animals exposed for 24 h, and over time a G0/G1 phase blockage. The effects of CCA in S-phase were similar, but more severe than those of As2O5. Since environmental and public health hazards due to the long durability of CCA-treated wood products, these data confirm that CCA has profoundly toxic effects on cell cycle, distinct from the compounds themselves. These cytostatic effects support cell cycle dynamics as a valuable endpoint to assess the toxicity of remaining CCA-treated infrastructures, and the expected increased waste stream over the coming decades. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01T00:00:00Z 2020-01 2023-04-12T14:03:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/36946 |
url |
http://hdl.handle.net/10773/36946 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1382-6689 10.1016/j.etap.2019.103297 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137730564718592 |