Expression of fibroblast growth factor 8 isoforms in the breast cancer cell line MDA-MB-231 and malignant and non-malignant breast tissue

• Autor(a) principal: Inês Isabel Carvalho Guedes
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.22/16704
• topic: Breast cancer; Proliferation; Migration

Breast cancer represents a quartar of all cancers diagnosed in women. Fibroblast growth factor 8 (FGF8) plays an important role in embryogenesis and it is involved in the development and differentiation of different organs. the level of FGF8 in normal adult tissues is very low or non-detectable but it has been reported to be expressed at elevated levels in some malignancies ncluding breast cancer. To evaluate the effects of FGF8 on breast cancer cells, proliferation and migration od MDA-MB-231 cells transfectef with FGF8 isoforms a, b and e in comparison with control transfected cells were studied. The expression of the FGF8 isoforms breast tissue using qPCR was also studied. The results showed an increased level of each FGF8 isoform in transfected cells compared to mock-transfected cells. Rate of proliferation was higher in the cells transfected with FGF8e than in control cells. The results also demonstrated that the rate of migration was higher in MDA-MB-231 FGF8a cells than in control cells. Expression of FGF8b and FGF8e was detected in a small number of breast cancer tissues and non-malignant breast tissues. However, demonstration of FGF8b and FGF8e expression in breast and breast cancer tissue warrants further studies to understand the role of FGF8 isoforms in breast cancer. Due to the heterogneity of breast cancer tissue, a large number of tumor samples would be needed for further analyses.