Expression of fibroblast growth factor 8 isoforms in the breast cancer cell line MDA-MB-231 and malignant and non-malignant breast tissue
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/16704 |
Resumo: | Breast cancer represents a quartar of all cancers diagnosed in women. Fibroblast growth factor 8 (FGF8) plays an important role in embryogenesis and it is involved in the development and differentiation of different organs. the level of FGF8 in normal adult tissues is very low or non-detectable but it has been reported to be expressed at elevated levels in some malignancies ncluding breast cancer. To evaluate the effects of FGF8 on breast cancer cells, proliferation and migration od MDA-MB-231 cells transfectef with FGF8 isoforms a, b and e in comparison with control transfected cells were studied. The expression of the FGF8 isoforms breast tissue using qPCR was also studied. The results showed an increased level of each FGF8 isoform in transfected cells compared to mock-transfected cells. Rate of proliferation was higher in the cells transfected with FGF8e than in control cells. The results also demonstrated that the rate of migration was higher in MDA-MB-231 FGF8a cells than in control cells. Expression of FGF8b and FGF8e was detected in a small number of breast cancer tissues and non-malignant breast tissues. However, demonstration of FGF8b and FGF8e expression in breast and breast cancer tissue warrants further studies to understand the role of FGF8 isoforms in breast cancer. Due to the heterogneity of breast cancer tissue, a large number of tumor samples would be needed for further analyses. |
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Expression of fibroblast growth factor 8 isoforms in the breast cancer cell line MDA-MB-231 and malignant and non-malignant breast tissueBreast cancerProliferationMigrationBreast cancer represents a quartar of all cancers diagnosed in women. Fibroblast growth factor 8 (FGF8) plays an important role in embryogenesis and it is involved in the development and differentiation of different organs. the level of FGF8 in normal adult tissues is very low or non-detectable but it has been reported to be expressed at elevated levels in some malignancies ncluding breast cancer. To evaluate the effects of FGF8 on breast cancer cells, proliferation and migration od MDA-MB-231 cells transfectef with FGF8 isoforms a, b and e in comparison with control transfected cells were studied. The expression of the FGF8 isoforms breast tissue using qPCR was also studied. The results showed an increased level of each FGF8 isoform in transfected cells compared to mock-transfected cells. Rate of proliferation was higher in the cells transfected with FGF8e than in control cells. The results also demonstrated that the rate of migration was higher in MDA-MB-231 FGF8a cells than in control cells. Expression of FGF8b and FGF8e was detected in a small number of breast cancer tissues and non-malignant breast tissues. However, demonstration of FGF8b and FGF8e expression in breast and breast cancer tissue warrants further studies to understand the role of FGF8 isoforms in breast cancer. Due to the heterogneity of breast cancer tissue, a large number of tumor samples would be needed for further analyses.O cancro da mama representa um quarto de todos os cancros diagnosticados em mulheres. O fator de crescimento fibroblástico 8 (FGF8) tem um papel importante na embriogénese e está envolvido no desenvolvimento e diferenciação de diferentes órgãos. Apesar de níveis baixos terem sido detetados em tecidos hormonais, foi descrito que o FGF8 é expresso em níveis elevados no cancro da mama. Para avaliar os efeitos do FGF8 nas células do cancro da mama, foi estudada a proliferação e migração de células MDA-MB-231 transformadas com as isoformas a, b e e em comparação a células controlo. A expressão das isoformas de FGF8 em tecido mamário foi estudada por qPCR. Os resultados mostraram níveis aumentados de células transformadas com isoformas de FGF8 quando comparadas com células controlo. A taxa de proliferação foi mais elevada em células transformadas com a isoforma e. Os resultados também demonstraram que a taxa de migração foi mais elevada em células MDA-MB-231FGF8a quando comparada com células controlo. Baixos níveis de FGF8b e FGF8e foram detetadas num pequeno número de tecidos mamários malignos e não malignos. No entanto, a demonstração da expressão de FGF8b e e em tecido maligno e não maligno justifica futuro estudos para entender o papel das isoformas de FGF8 no cancro da mama. Devido à heterogeneidade do tecido mamário, mais amostras precisam de ser estudadas.Härkonën, PirkkoPrudêncio, CristinaVieira, MónicaRepositório Científico do Instituto Politécnico do PortoInês Isabel Carvalho Guedes2021-01-19T12:43:42Z2019-12-022019-12-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.22/16704TID:202445739enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:03:52Zoai:recipp.ipp.pt:10400.22/16704Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:36:20.599368Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Expression of fibroblast growth factor 8 isoforms in the breast cancer cell line MDA-MB-231 and malignant and non-malignant breast tissue |
title |
Expression of fibroblast growth factor 8 isoforms in the breast cancer cell line MDA-MB-231 and malignant and non-malignant breast tissue |
spellingShingle |
Expression of fibroblast growth factor 8 isoforms in the breast cancer cell line MDA-MB-231 and malignant and non-malignant breast tissue Inês Isabel Carvalho Guedes Breast cancer Proliferation Migration |
title_short |
Expression of fibroblast growth factor 8 isoforms in the breast cancer cell line MDA-MB-231 and malignant and non-malignant breast tissue |
title_full |
Expression of fibroblast growth factor 8 isoforms in the breast cancer cell line MDA-MB-231 and malignant and non-malignant breast tissue |
title_fullStr |
Expression of fibroblast growth factor 8 isoforms in the breast cancer cell line MDA-MB-231 and malignant and non-malignant breast tissue |
title_full_unstemmed |
Expression of fibroblast growth factor 8 isoforms in the breast cancer cell line MDA-MB-231 and malignant and non-malignant breast tissue |
title_sort |
Expression of fibroblast growth factor 8 isoforms in the breast cancer cell line MDA-MB-231 and malignant and non-malignant breast tissue |
author |
Inês Isabel Carvalho Guedes |
author_facet |
Inês Isabel Carvalho Guedes |
author_role |
author |
dc.contributor.none.fl_str_mv |
Härkonën, Pirkko Prudêncio, Cristina Vieira, Mónica Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Inês Isabel Carvalho Guedes |
dc.subject.por.fl_str_mv |
Breast cancer Proliferation Migration |
topic |
Breast cancer Proliferation Migration |
description |
Breast cancer represents a quartar of all cancers diagnosed in women. Fibroblast growth factor 8 (FGF8) plays an important role in embryogenesis and it is involved in the development and differentiation of different organs. the level of FGF8 in normal adult tissues is very low or non-detectable but it has been reported to be expressed at elevated levels in some malignancies ncluding breast cancer. To evaluate the effects of FGF8 on breast cancer cells, proliferation and migration od MDA-MB-231 cells transfectef with FGF8 isoforms a, b and e in comparison with control transfected cells were studied. The expression of the FGF8 isoforms breast tissue using qPCR was also studied. The results showed an increased level of each FGF8 isoform in transfected cells compared to mock-transfected cells. Rate of proliferation was higher in the cells transfected with FGF8e than in control cells. The results also demonstrated that the rate of migration was higher in MDA-MB-231 FGF8a cells than in control cells. Expression of FGF8b and FGF8e was detected in a small number of breast cancer tissues and non-malignant breast tissues. However, demonstration of FGF8b and FGF8e expression in breast and breast cancer tissue warrants further studies to understand the role of FGF8 isoforms in breast cancer. Due to the heterogneity of breast cancer tissue, a large number of tumor samples would be needed for further analyses. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-02 2019-12-02T00:00:00Z 2021-01-19T12:43:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/16704 TID:202445739 |
url |
http://hdl.handle.net/10400.22/16704 |
identifier_str_mv |
TID:202445739 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131454821629952 |