Origin and epidemiological history of HIV-1 CRF14_BG
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/59101 |
Resumo: | Background CRF14_BG isolates, originally found in Spain, are characterized by CXCR4 tropism and rapid disease progression. This study aimed to identify the origin of CRF14_BG and reconstruct its epidemiological history based on new isolates from Portugal. Methodology/Principal Findings C2V3C3 env gene sequences were obtained from 62 samples collected in 1993–1998 from Portuguese HIV-1 patients. Full-length genomic sequences were obtained from three patients. Viral subtypes, diversity, divergence rate and positive selection were investigated by phylogenetic analysis. The molecular structure of the genomes was determined by bootscanning. A relaxed molecular clock model was used to date the origin of CRF14_BG. Geno2pheno was used to predict viral tropism. Subtype B was the most prevalent subtype (45 sequences; 73%) followed by CRF14_BG (8; 13%), G (4; 6%), F1 (2; 3%), C (2; 3%) and CRF02_AG (1; 2%). Three CRF14_BG sequences were derived from 1993 samples. Near full-length genomic sequences were strongly related to the CRF14_BG isolates from Spain. Genetic diversity of the Portuguese isolates was significantly higher than the Spanish isolates (0.044 vs 0.014, P<0.0001). The mean date of origin of the CRF14_BG cluster was estimated to be 1992 (range, 1989 and 1996) based on the subtype G genomic region and 1989 (range, 1984–1993) based on the subtype B genomic region. Most CRF14_BG strains (78.9%) were predicted to be CXCR4. Finally, up to five amino acids were under selective pressure in subtype B V3 loop whereas only one was found in the CRF14_BG cluster. Conclusions CRF14_BG emerged in Portugal in the early 1990 s soon after the beginning of the HIV-1 epidemics, spread to Spain in late 1990 s as a consequence of IVDUs migration and then to the rest of Europe. CXCR4 tropism is a general characteristic of this CRF that may have been selected for by escape from neutralizing antibody response. |
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Origin and epidemiological history of HIV-1 CRF14_BGBackground CRF14_BG isolates, originally found in Spain, are characterized by CXCR4 tropism and rapid disease progression. This study aimed to identify the origin of CRF14_BG and reconstruct its epidemiological history based on new isolates from Portugal. Methodology/Principal Findings C2V3C3 env gene sequences were obtained from 62 samples collected in 1993–1998 from Portuguese HIV-1 patients. Full-length genomic sequences were obtained from three patients. Viral subtypes, diversity, divergence rate and positive selection were investigated by phylogenetic analysis. The molecular structure of the genomes was determined by bootscanning. A relaxed molecular clock model was used to date the origin of CRF14_BG. Geno2pheno was used to predict viral tropism. Subtype B was the most prevalent subtype (45 sequences; 73%) followed by CRF14_BG (8; 13%), G (4; 6%), F1 (2; 3%), C (2; 3%) and CRF02_AG (1; 2%). Three CRF14_BG sequences were derived from 1993 samples. Near full-length genomic sequences were strongly related to the CRF14_BG isolates from Spain. Genetic diversity of the Portuguese isolates was significantly higher than the Spanish isolates (0.044 vs 0.014, P<0.0001). The mean date of origin of the CRF14_BG cluster was estimated to be 1992 (range, 1989 and 1996) based on the subtype G genomic region and 1989 (range, 1984–1993) based on the subtype B genomic region. Most CRF14_BG strains (78.9%) were predicted to be CXCR4. Finally, up to five amino acids were under selective pressure in subtype B V3 loop whereas only one was found in the CRF14_BG cluster. Conclusions CRF14_BG emerged in Portugal in the early 1990 s soon after the beginning of the HIV-1 epidemics, spread to Spain in late 1990 s as a consequence of IVDUs migration and then to the rest of Europe. CXCR4 tropism is a general characteristic of this CRF that may have been selected for by escape from neutralizing antibody response.This work was supported by grant PTDC/SAU-FCF/67673/2006 from Fundação para a Ciência e Tecnologia Portugal, and by CHAIN (Collaborative HIV and Anti-HIV Drug Resistance Network), European Union. Inês Bártolo and Pedro Borrego are recipients of PhD scholarships from Fundação para a Ciência e Tecnologia (FCT), Portugal. Ana Abecasis is supported by a Post-Doc grant from the Fundação para a Ciência e Tecnologia (FCT), Portugal (SFRH/BPD/65605/2009). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.PlosRepositório da Universidade de LisboaBártolo, InêsAbecasis, Ana B.Borrego, PedroBarroso, HelenaMcCutchan, FrancineGomes, PerpetuaCamacho, RicardoTaveira, Nuno2023-08-31T12:20:25Z20112023-02-03T14:38:39Z2011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/59101engBártolo I, Abecasis AB, Borrego P, Barroso H, McCutchan F, Gomes P, et al. Origin and epidemiological history of hiv-1 crf14_bg. PLOS ONE [Internet]. 28 de setembro de 2011;6(9):e24130. Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0024130cv-prod-110846110.1371/journal.pone.00241302-s2.0-80053252119info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:03:34Zoai:repositorio.ul.pt:10451/59101Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:06:42.579670Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Origin and epidemiological history of HIV-1 CRF14_BG |
title |
Origin and epidemiological history of HIV-1 CRF14_BG |
spellingShingle |
Origin and epidemiological history of HIV-1 CRF14_BG Bártolo, Inês |
title_short |
Origin and epidemiological history of HIV-1 CRF14_BG |
title_full |
Origin and epidemiological history of HIV-1 CRF14_BG |
title_fullStr |
Origin and epidemiological history of HIV-1 CRF14_BG |
title_full_unstemmed |
Origin and epidemiological history of HIV-1 CRF14_BG |
title_sort |
Origin and epidemiological history of HIV-1 CRF14_BG |
author |
Bártolo, Inês |
author_facet |
Bártolo, Inês Abecasis, Ana B. Borrego, Pedro Barroso, Helena McCutchan, Francine Gomes, Perpetua Camacho, Ricardo Taveira, Nuno |
author_role |
author |
author2 |
Abecasis, Ana B. Borrego, Pedro Barroso, Helena McCutchan, Francine Gomes, Perpetua Camacho, Ricardo Taveira, Nuno |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Bártolo, Inês Abecasis, Ana B. Borrego, Pedro Barroso, Helena McCutchan, Francine Gomes, Perpetua Camacho, Ricardo Taveira, Nuno |
description |
Background CRF14_BG isolates, originally found in Spain, are characterized by CXCR4 tropism and rapid disease progression. This study aimed to identify the origin of CRF14_BG and reconstruct its epidemiological history based on new isolates from Portugal. Methodology/Principal Findings C2V3C3 env gene sequences were obtained from 62 samples collected in 1993–1998 from Portuguese HIV-1 patients. Full-length genomic sequences were obtained from three patients. Viral subtypes, diversity, divergence rate and positive selection were investigated by phylogenetic analysis. The molecular structure of the genomes was determined by bootscanning. A relaxed molecular clock model was used to date the origin of CRF14_BG. Geno2pheno was used to predict viral tropism. Subtype B was the most prevalent subtype (45 sequences; 73%) followed by CRF14_BG (8; 13%), G (4; 6%), F1 (2; 3%), C (2; 3%) and CRF02_AG (1; 2%). Three CRF14_BG sequences were derived from 1993 samples. Near full-length genomic sequences were strongly related to the CRF14_BG isolates from Spain. Genetic diversity of the Portuguese isolates was significantly higher than the Spanish isolates (0.044 vs 0.014, P<0.0001). The mean date of origin of the CRF14_BG cluster was estimated to be 1992 (range, 1989 and 1996) based on the subtype G genomic region and 1989 (range, 1984–1993) based on the subtype B genomic region. Most CRF14_BG strains (78.9%) were predicted to be CXCR4. Finally, up to five amino acids were under selective pressure in subtype B V3 loop whereas only one was found in the CRF14_BG cluster. Conclusions CRF14_BG emerged in Portugal in the early 1990 s soon after the beginning of the HIV-1 epidemics, spread to Spain in late 1990 s as a consequence of IVDUs migration and then to the rest of Europe. CXCR4 tropism is a general characteristic of this CRF that may have been selected for by escape from neutralizing antibody response. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2011-01-01T00:00:00Z 2023-08-31T12:20:25Z 2023-02-03T14:38:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/59101 |
url |
http://hdl.handle.net/10451/59101 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bártolo I, Abecasis AB, Borrego P, Barroso H, McCutchan F, Gomes P, et al. Origin and epidemiological history of hiv-1 crf14_bg. PLOS ONE [Internet]. 28 de setembro de 2011;6(9):e24130. Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0024130 cv-prod-1108461 10.1371/journal.pone.0024130 2-s2.0-80053252119 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Plos |
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Plos |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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