The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains

Detalhes bibliográficos
Autor(a) principal: Guimarães, Monick L.
Data de Publicação: 2012
Outros Autores: Velarde-Dunois, Ketty G., Segurondo, David, Morgado, Mariza G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15815
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Guimarães, Monick L.Velarde-Dunois, Ketty G.Segurondo, DavidMorgado, Mariza G.2016-09-15T15:36:31Z2016-09-15T15:36:31Z2012GUIMARÃES, Monick L. et al. The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains. Virology Journal, v.9:19, 7p, 2012.1743-422Xhttps://www.arca.fiocruz.br/handle/icict/158151743-422XengBioMed CentralHIV-1BolíviaSubtiposHIV-1SubtypesBoliviaCRFThe HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Policía Nacional. La Paz, Bolivia.Ministerio de la Salud. Programa Departamental de ITS, VIH/SIDA. La Paz, Bolivia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Background: Molecular epidemiological studies of HIV-1 in South America have revealed the occurrence of subtypes B, F1 and BF1 recombinants. Even so, little information concerning the HIV-1 molecular epidemiology in Bolivia is available. In this study we performed phylogenetic analyses from samples collected in Bolivia at two different points in time over a 10 year span. We analyzed these samples to estimate the trends in the HIV subtype and recombinant forms over time. Materials and methods: Fifty one HIV-1 positive samples were collected in Bolivia over two distinct periods (1996 and 2005). These samples were genetically characterized based on partial pol protease/reverse transcriptase (pr/rt) and env regions. Alignment and neighbor-joining (NJ) phylogenetic analyses were established from partial env (n = 37) and all pol sequences using Mega 4. The remaining 14 env sequences from 1996 were previously characterized based on HMA-env (Heteroduplex mobility assay). The Simplot v.3.5.1 program was used to verify intragenic recombination, and SplitsTree 4.0 was employed to confirm the phylogenetic relationship of the BF1 recombinant samples. Results: Phylogenetic analysis of both env and pol regions confirmed the predominance of “pure” subtype B (72.5%) samples circulating in Bolivia and revealed a high prevalence of BF1 genotypes (27.5%). Eleven out of 14 BF1 recombinants displayed a mosaic structure identical or similar to that described for the CRF12_BF variant, one sample was classified as CRF17_BF, and two others were F1pol/Benv. No “pure” HIV-1 subtype F1 or B” variant of subtype B was detected in the present study. Of note, samples characterized as CRF12_BF-related were depicted only in 2005. Conclusion: HIV-1 genetic diversity in Bolivia is mostly driven by subtype B followed by BF1 recombinant strains from the CRF12_BF “family”. No significant temporal changes were detected between the mid-1990s and the mid- 2000s for subtype B (76.2% vs 70.0%) or BF1 recombinant (23.8% vs 30.0%) samples from Bolivia.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15815/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmonick2_guimaraew_etal_IOC_2012.pdfmonick2_guimaraew_etal_IOC_2012.pdfapplication/pdf515424https://www.arca.fiocruz.br/bitstream/icict/15815/2/monick2_guimaraew_etal_IOC_2012.pdffd3e8f2b8bff48ae4804c30f13466e89MD52TEXTmonick2_guimaraew_etal_IOC_2012.pdf.txtmonick2_guimaraew_etal_IOC_2012.pdf.txtExtracted texttext/plain31740https://www.arca.fiocruz.br/bitstream/icict/15815/3/monick2_guimaraew_etal_IOC_2012.pdf.txte5d2927963d00df1fecbb0f357387212MD53icict/158152018-04-02 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dc.title.pt_BR.fl_str_mv The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains
title The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains
spellingShingle The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains
Guimarães, Monick L.
HIV-1
Bolívia
Subtipos
HIV-1
Subtypes
Bolivia
CRF
title_short The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains
title_full The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains
title_fullStr The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains
title_full_unstemmed The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains
title_sort The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains
author Guimarães, Monick L.
author_facet Guimarães, Monick L.
Velarde-Dunois, Ketty G.
Segurondo, David
Morgado, Mariza G.
author_role author
author2 Velarde-Dunois, Ketty G.
Segurondo, David
Morgado, Mariza G.
author2_role author
author
author
dc.contributor.author.fl_str_mv Guimarães, Monick L.
Velarde-Dunois, Ketty G.
Segurondo, David
Morgado, Mariza G.
dc.subject.other.pt_BR.fl_str_mv HIV-1
Bolívia
Subtipos
topic HIV-1
Bolívia
Subtipos
HIV-1
Subtypes
Bolivia
CRF
dc.subject.en.pt_BR.fl_str_mv HIV-1
Subtypes
Bolivia
CRF
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2016-09-15T15:36:31Z
dc.date.available.fl_str_mv 2016-09-15T15:36:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUIMARÃES, Monick L. et al. The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains. Virology Journal, v.9:19, 7p, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15815
dc.identifier.issn.pt_BR.fl_str_mv 1743-422X
dc.identifier.eissn.none.fl_str_mv 1743-422X
identifier_str_mv GUIMARÃES, Monick L. et al. The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains. Virology Journal, v.9:19, 7p, 2012.
1743-422X
url https://www.arca.fiocruz.br/handle/icict/15815
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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