Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522004000200006 |
Resumo: | The diagnosis of Armillaria, a genus including distinct species of highly woody plant-pathogenic root-infecting fungi with worldwide distribution, is usually based on morphological characteristics and mating-tests, although the PCR-based restriction fragment length polymorphism (RFLP-PCR), specifically in nuclear rDNA spacers, have also been applied. In the present study, mating-tests and restriction analysis of Internal Transcribed Spacer 1 (ITS1) were used to identify 20 isolates of a Portuguese Armillaria collection. Although the majority of the diploid isolates (80%) could be identified in diploid-haploid pairings, the method is laborious, takes too much time (up to 2 months), and presents a high rate of inconclusive results. The ITS1 region showed to be a reliable molecular marker for A. mellea, in particular when HinfI restriction analysis is applied, since two fragments with 245 bp and 125 bp have been obtained for this most aggressive species whereas 290 bp and 70 bp were produced from isolates of the other European species. As simple molecular techniques are involved and the whole procedure can be performed in one day, A. mellea identification by ITS1 analysis is a clearly accessible and more advantageous tool to plant pathology laboratories, mainly those involved on the control and preservation of forest trees. |
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Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNARFLP-PCRDNAArmillaria melleawoody plantscork oakThe diagnosis of Armillaria, a genus including distinct species of highly woody plant-pathogenic root-infecting fungi with worldwide distribution, is usually based on morphological characteristics and mating-tests, although the PCR-based restriction fragment length polymorphism (RFLP-PCR), specifically in nuclear rDNA spacers, have also been applied. In the present study, mating-tests and restriction analysis of Internal Transcribed Spacer 1 (ITS1) were used to identify 20 isolates of a Portuguese Armillaria collection. Although the majority of the diploid isolates (80%) could be identified in diploid-haploid pairings, the method is laborious, takes too much time (up to 2 months), and presents a high rate of inconclusive results. The ITS1 region showed to be a reliable molecular marker for A. mellea, in particular when HinfI restriction analysis is applied, since two fragments with 245 bp and 125 bp have been obtained for this most aggressive species whereas 290 bp and 70 bp were produced from isolates of the other European species. As simple molecular techniques are involved and the whole procedure can be performed in one day, A. mellea identification by ITS1 analysis is a clearly accessible and more advantageous tool to plant pathology laboratories, mainly those involved on the control and preservation of forest trees.Unidade de Silvicultura e Produtos Florestais2004-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522004000200006Silva Lusitana v.12 n.1 2004reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522004000200006Bragança,HelenaTenreiro,RogérioSantos,Natérciainfo:eu-repo/semantics/openAccess2024-02-06T16:59:04Zoai:scielo:S0870-63522004000200006Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:15:32.813003Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA |
title |
Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA |
spellingShingle |
Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA Bragança,Helena RFLP-PCR DNA Armillaria mellea woody plants cork oak |
title_short |
Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA |
title_full |
Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA |
title_fullStr |
Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA |
title_full_unstemmed |
Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA |
title_sort |
Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA |
author |
Bragança,Helena |
author_facet |
Bragança,Helena Tenreiro,Rogério Santos,Natércia |
author_role |
author |
author2 |
Tenreiro,Rogério Santos,Natércia |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Bragança,Helena Tenreiro,Rogério Santos,Natércia |
dc.subject.por.fl_str_mv |
RFLP-PCR DNA Armillaria mellea woody plants cork oak |
topic |
RFLP-PCR DNA Armillaria mellea woody plants cork oak |
description |
The diagnosis of Armillaria, a genus including distinct species of highly woody plant-pathogenic root-infecting fungi with worldwide distribution, is usually based on morphological characteristics and mating-tests, although the PCR-based restriction fragment length polymorphism (RFLP-PCR), specifically in nuclear rDNA spacers, have also been applied. In the present study, mating-tests and restriction analysis of Internal Transcribed Spacer 1 (ITS1) were used to identify 20 isolates of a Portuguese Armillaria collection. Although the majority of the diploid isolates (80%) could be identified in diploid-haploid pairings, the method is laborious, takes too much time (up to 2 months), and presents a high rate of inconclusive results. The ITS1 region showed to be a reliable molecular marker for A. mellea, in particular when HinfI restriction analysis is applied, since two fragments with 245 bp and 125 bp have been obtained for this most aggressive species whereas 290 bp and 70 bp were produced from isolates of the other European species. As simple molecular techniques are involved and the whole procedure can be performed in one day, A. mellea identification by ITS1 analysis is a clearly accessible and more advantageous tool to plant pathology laboratories, mainly those involved on the control and preservation of forest trees. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522004000200006 |
url |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522004000200006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522004000200006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Unidade de Silvicultura e Produtos Florestais |
publisher.none.fl_str_mv |
Unidade de Silvicultura e Produtos Florestais |
dc.source.none.fl_str_mv |
Silva Lusitana v.12 n.1 2004 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799137255211663360 |