Paper-based in-situ gold nanoparticle synthesis for colorimetric, non-enzymatic glucose level determination

Detalhes bibliográficos
Autor(a) principal: Pinheiro, Tomás
Data de Publicação: 2020
Outros Autores: Ferrão, João, Marques, Ana Carolina, Oliveira, Maria J., Batra, Nitin M., Costa, Pedro M. F. J., Macedo, M. Paula, Águas, Hugo, Martins, Rodrigo, Fortunato, Elvira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/117136
Resumo: ref. 787410 SFRH/BD/115173/2016 SFRH/BD/132057/2017
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spelling Paper-based in-situ gold nanoparticle synthesis for colorimetric, non-enzymatic glucose level determinationColorimetricDiabetesEnzyme-freeGlucoseGold nanoparticlesIn-situ synthesisPaper-based platformChemical Engineering(all)Materials Science(all)SDG 3 - Good Health and Well-beingref. 787410 SFRH/BD/115173/2016 SFRH/BD/132057/2017Due to its properties, paper represents an alternative to perform point-of-care tests for colorimetric determination of glucose levels, providing simple, rapid, and inexpensive means of diagnosis. In this work, we report the development of a novel, rapid, disposable, inexpensive, enzyme-free, and colorimetric paper-based assay for glucose level determination. This sensing strategy is based on the synthesis of gold nanoparticles (AuNPs) by reduction of a gold salt precursor, in which glucose acts simultaneously as reducing and capping agent. This leads to a direct measurement of glucose without any enzymes or depending on the detection of intermediate products as in conventional enzymatic colorimetric methods. Firstly, we modelled the synthesis reaction of AuNPs to determine the optical, morphological, and kinetic properties and their manipulation for glucose sensing, by determining the influence of each of the reaction precursors towards the produced AuNPs, providing a guide for the manipulation of nucleation and growth. The adaptation of this synthesis into the developed paper platform was tested and calibrated using different standard solutions with physiological concentrations of glucose. The response of the colorimetric signals obtained with this paper-based platform showed a linear behavior until 20 mM, required for glycemic control in diabetes, using the Red × Value/Grey feature combination as a calibration metric, to describe the variations in color intensity and hue in the spot test zone. The colorimetric sensor revealed a detection limit of 0.65 mM, depending on calibration metric and sensitivity of 0.013 AU/mM for a linear sensitivity range from 1.25 to 20 mM, with high specificity for the determination of glucose in complex standards with other common reducing interferents and human serum.DCM - Departamento de Ciência dos MateriaisCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNPinheiro, TomásFerrão, JoãoMarques, Ana CarolinaOliveira, Maria J.Batra, Nitin M.Costa, Pedro M. F. J.Macedo, M. PaulaÁguas, HugoMartins, RodrigoFortunato, Elvira2021-05-05T23:24:50Z2020-10-142020-10-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20application/pdfhttp://hdl.handle.net/10362/117136eng2079-4991PURE: 26294877https://doi.org/10.3390/nano10102027info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:00:10Zoai:run.unl.pt:10362/117136Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:29.439509Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Paper-based in-situ gold nanoparticle synthesis for colorimetric, non-enzymatic glucose level determination
title Paper-based in-situ gold nanoparticle synthesis for colorimetric, non-enzymatic glucose level determination
spellingShingle Paper-based in-situ gold nanoparticle synthesis for colorimetric, non-enzymatic glucose level determination
Pinheiro, Tomás
Colorimetric
Diabetes
Enzyme-free
Glucose
Gold nanoparticles
In-situ synthesis
Paper-based platform
Chemical Engineering(all)
Materials Science(all)
SDG 3 - Good Health and Well-being
title_short Paper-based in-situ gold nanoparticle synthesis for colorimetric, non-enzymatic glucose level determination
title_full Paper-based in-situ gold nanoparticle synthesis for colorimetric, non-enzymatic glucose level determination
title_fullStr Paper-based in-situ gold nanoparticle synthesis for colorimetric, non-enzymatic glucose level determination
title_full_unstemmed Paper-based in-situ gold nanoparticle synthesis for colorimetric, non-enzymatic glucose level determination
title_sort Paper-based in-situ gold nanoparticle synthesis for colorimetric, non-enzymatic glucose level determination
author Pinheiro, Tomás
author_facet Pinheiro, Tomás
Ferrão, João
Marques, Ana Carolina
Oliveira, Maria J.
Batra, Nitin M.
Costa, Pedro M. F. J.
Macedo, M. Paula
Águas, Hugo
Martins, Rodrigo
Fortunato, Elvira
author_role author
author2 Ferrão, João
Marques, Ana Carolina
Oliveira, Maria J.
Batra, Nitin M.
Costa, Pedro M. F. J.
Macedo, M. Paula
Águas, Hugo
Martins, Rodrigo
Fortunato, Elvira
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCM - Departamento de Ciência dos Materiais
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Pinheiro, Tomás
Ferrão, João
Marques, Ana Carolina
Oliveira, Maria J.
Batra, Nitin M.
Costa, Pedro M. F. J.
Macedo, M. Paula
Águas, Hugo
Martins, Rodrigo
Fortunato, Elvira
dc.subject.por.fl_str_mv Colorimetric
Diabetes
Enzyme-free
Glucose
Gold nanoparticles
In-situ synthesis
Paper-based platform
Chemical Engineering(all)
Materials Science(all)
SDG 3 - Good Health and Well-being
topic Colorimetric
Diabetes
Enzyme-free
Glucose
Gold nanoparticles
In-situ synthesis
Paper-based platform
Chemical Engineering(all)
Materials Science(all)
SDG 3 - Good Health and Well-being
description ref. 787410 SFRH/BD/115173/2016 SFRH/BD/132057/2017
publishDate 2020
dc.date.none.fl_str_mv 2020-10-14
2020-10-14T00:00:00Z
2021-05-05T23:24:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/117136
url http://hdl.handle.net/10362/117136
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2079-4991
PURE: 26294877
https://doi.org/10.3390/nano10102027
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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