Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and Release

Detalhes bibliográficos
Autor(a) principal: Barroso, Telma
Data de Publicação: 2012
Outros Autores: Roque, Ana Cecília Afonso, Ricardo, Ana Isabel Nobre Martins Aguiar de Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/20795
Resumo: The authors would like to thank the financial support from Fundacao para a Ciencia e Tecnologia, Portugal, through contracts MIT-Pt/BS-CTRM/0051/2008, PTDC/EBB-BIO/102163/2008, PTDC/EBB-BIO/098961/2008 and PTDC/EBB-BIO/118317/2010 and doctoral grant SFRH/BD/62475/2009 (T. B.), Bioengineering Systems Focus Area, Fundacao Calouste Gulbenkian, FEDER and FSE. We wish to thank the Analytical Services Laboratory of REQUIMTE for the characterization of materials. Authors are also thankful to Lonza Biologics, UK (Dr Richard Alldread) and the Animal Cell Technology Unit of ITQB-UNL/IBET (Dr Paula M Alves and Dr Ana Teixeira) for providing the cell culture bulks of antibodies.
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spelling Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and ReleaseAFFINITY-CHROMATOGRAPHYMECHANICAL-PROPERTIESBIOMIMETIC LIGANDSBINDING LIGANDSCAFFOLDSNANOPARTICLES;PURIFICATIONSEPARATIONIMMOBILIZATIONPERFORMANCEThe authors would like to thank the financial support from Fundacao para a Ciencia e Tecnologia, Portugal, through contracts MIT-Pt/BS-CTRM/0051/2008, PTDC/EBB-BIO/102163/2008, PTDC/EBB-BIO/098961/2008 and PTDC/EBB-BIO/118317/2010 and doctoral grant SFRH/BD/62475/2009 (T. B.), Bioengineering Systems Focus Area, Fundacao Calouste Gulbenkian, FEDER and FSE. We wish to thank the Analytical Services Laboratory of REQUIMTE for the characterization of materials. Authors are also thankful to Lonza Biologics, UK (Dr Richard Alldread) and the Animal Cell Technology Unit of ITQB-UNL/IBET (Dr Paula M Alves and Dr Ana Teixeira) for providing the cell culture bulks of antibodies.Chitosan-based monoliths activated by plasma technology induced the coupling of a robust biomimetic ligand, previously reported as an artificial Protein A, with high yields while minimizing the environmental impact of the procedure. Due to the high porosity, good mechanical and tunable physicochemical properties of the affinity chitosan-based monoliths, it is possible to achieve high binding capacities (150 ± 10 mg antibody per gram support), and to recover 90 ± 5% of the bound protein with 98% purity directly from cell-culture extracts. Therefore, the chitosan-based monoliths prepared by clean processes exhibit a remarkable performance for the one-step capture and recovery of pure antibodies or other biological molecules with biopharmaceutical relevance.CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)DQ - Departamento de QuímicaRUNBarroso, TelmaRoque, Ana Cecília AfonsoRicardo, Ana Isabel Nobre Martins Aguiar de Oliveira2017-05-30T13:57:29Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/20795eng2046-2069PURE: 401898https://doi.org/10.1039/C2RA21687Finfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:06:39Zoai:run.unl.pt:10362/20795Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:26:28.952048Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and Release
title Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and Release
spellingShingle Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and Release
Barroso, Telma
AFFINITY-CHROMATOGRAPHY
MECHANICAL-PROPERTIES
BIOMIMETIC LIGANDS
BINDING LIGAND
SCAFFOLDS
NANOPARTICLES;
PURIFICATION
SEPARATION
IMMOBILIZATION
PERFORMANCE
title_short Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and Release
title_full Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and Release
title_fullStr Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and Release
title_full_unstemmed Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and Release
title_sort Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and Release
author Barroso, Telma
author_facet Barroso, Telma
Roque, Ana Cecília Afonso
Ricardo, Ana Isabel Nobre Martins Aguiar de Oliveira
author_role author
author2 Roque, Ana Cecília Afonso
Ricardo, Ana Isabel Nobre Martins Aguiar de Oliveira
author2_role author
author
dc.contributor.none.fl_str_mv CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Barroso, Telma
Roque, Ana Cecília Afonso
Ricardo, Ana Isabel Nobre Martins Aguiar de Oliveira
dc.subject.por.fl_str_mv AFFINITY-CHROMATOGRAPHY
MECHANICAL-PROPERTIES
BIOMIMETIC LIGANDS
BINDING LIGAND
SCAFFOLDS
NANOPARTICLES;
PURIFICATION
SEPARATION
IMMOBILIZATION
PERFORMANCE
topic AFFINITY-CHROMATOGRAPHY
MECHANICAL-PROPERTIES
BIOMIMETIC LIGANDS
BINDING LIGAND
SCAFFOLDS
NANOPARTICLES;
PURIFICATION
SEPARATION
IMMOBILIZATION
PERFORMANCE
description The authors would like to thank the financial support from Fundacao para a Ciencia e Tecnologia, Portugal, through contracts MIT-Pt/BS-CTRM/0051/2008, PTDC/EBB-BIO/102163/2008, PTDC/EBB-BIO/098961/2008 and PTDC/EBB-BIO/118317/2010 and doctoral grant SFRH/BD/62475/2009 (T. B.), Bioengineering Systems Focus Area, Fundacao Calouste Gulbenkian, FEDER and FSE. We wish to thank the Analytical Services Laboratory of REQUIMTE for the characterization of materials. Authors are also thankful to Lonza Biologics, UK (Dr Richard Alldread) and the Animal Cell Technology Unit of ITQB-UNL/IBET (Dr Paula M Alves and Dr Ana Teixeira) for providing the cell culture bulks of antibodies.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2017-05-30T13:57:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/20795
url http://hdl.handle.net/10362/20795
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2046-2069
PURE: 401898
https://doi.org/10.1039/C2RA21687F
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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