Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles

Detalhes bibliográficos
Autor(a) principal: Graça, Ana L.
Data de Publicação: 2022
Outros Autores: Gomez-Florit, Manuel, Osório, Hugo, Rodrigues, Márcia T., Domingues, Rui Miguel Andrade, Reis, R. L., Gomes, Manuela E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/80589
Resumo: Extracellular vesicles (EVs) have emerged as cell-free nanotherapeutic agents for the potential treatment of multiple diseases and for tissue engineering and regenerative medicine strategies. Nevertheless, the field has typically relied on EVs derived from stem cells, the production of which in high quantities and high reproducibility is still under debate. Platelet-derived EVs were produced by a freeze–thaw method of platelet concentrates, a highly available clinical waste material. The aim of this study was to produce and thoroughly characterize platelet-derived EVs and understand their effects in adipose-tissue derived stem cells (hASCs), endothelial cells (HUVECs) and macrophages. Two different EV populations were obtained after differential centrifugation, namely small EVs (sEVs) and medium EVs (mEVs), which showed different size distributions and unique proteomic signatures. EV interaction with hASCs resulted in the modulation of the gene expression of markers related to their commitment toward different lineages. Moreover, mEVs showed higher angiogenic potential than sEVs, in a tube formation assay with HUVECs. Also, the EVs were able to modulate macrophage polarization. Altogether, these results suggest that platelet-derived EVs are promising candidates to be used as biochemical signals or therapeutic tools in tissue engineering and regenerative medicine approaches.
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spelling Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesiclesAngiogenesisExtracellular vesiclesInflammationPlateletsProteomicsStem cellsScience & TechnologyExtracellular vesicles (EVs) have emerged as cell-free nanotherapeutic agents for the potential treatment of multiple diseases and for tissue engineering and regenerative medicine strategies. Nevertheless, the field has typically relied on EVs derived from stem cells, the production of which in high quantities and high reproducibility is still under debate. Platelet-derived EVs were produced by a freeze–thaw method of platelet concentrates, a highly available clinical waste material. The aim of this study was to produce and thoroughly characterize platelet-derived EVs and understand their effects in adipose-tissue derived stem cells (hASCs), endothelial cells (HUVECs) and macrophages. Two different EV populations were obtained after differential centrifugation, namely small EVs (sEVs) and medium EVs (mEVs), which showed different size distributions and unique proteomic signatures. EV interaction with hASCs resulted in the modulation of the gene expression of markers related to their commitment toward different lineages. Moreover, mEVs showed higher angiogenic potential than sEVs, in a tube formation assay with HUVECs. Also, the EVs were able to modulate macrophage polarization. Altogether, these results suggest that platelet-derived EVs are promising candidates to be used as biochemical signals or therapeutic tools in tissue engineering and regenerative medicine approaches.The authors acknowledge ERC CoG MagTendon grant agreement 772817; EC Twinning project Achilles 810850; Fundação para a Ciência e a Tecnologia (FCT) for PhD grant PD/59/2013 and PD/BD/135255/2017, Post-Doc grant SFRH/BPD/112459/2015, CEECIND/01375/2017. Project NORTE-01-0145-FEDER-000021 supported by NORTE2020, under the PORTUGAL2020 Partnership, through the European Regional Development Found and the Portuguese Mass Spectrometry Network, integrated in the National Roadmap of Research Infrastructures of Strategic Relevance (ROTEIRO/0028/2013; LISBOA-01-0145-FEDER-022125). The authors also thank to the Plastic Surgery Department of Hospital da Prelada (Porto, Portugal) and Serviço de Imunohemoterapia do Centro Hospitalar de São João (CHUSJ; Porto, Portugal) for providing adipose tissue samples and platelet concentrates, respectively. Centro de Engenharia Biológica (CEB) of the University of Minho is acknowledged for allowing the use of the ultracentrifuge. Some figures were created with BioRender.com.Royal Society of ChemistryUniversidade do MinhoGraça, Ana L.Gomez-Florit, ManuelOsório, HugoRodrigues, Márcia T.Domingues, Rui Miguel AndradeReis, R. L.Gomes, Manuela E.2022-052022-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80589engGraça A. L., Gómez-Florit M., Osório H., Rodrigues M. T., Domingues R. M. A., Reis R. L., Gomes M. E. Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles, Nanoscale, Vol. 14, Issue 17, pp. 6543-6556, doi:10.1039/D1NR08108J, 20222040-33642040-337210.1039/D1NR08108J35420605https://pubs.rsc.org/en/content/articlelanding/2022/NR/D1NR08108Jinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:27Zoai:repositorium.sdum.uminho.pt:1822/80589Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:42:08.647401Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles
title Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles
spellingShingle Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles
Graça, Ana L.
Angiogenesis
Extracellular vesicles
Inflammation
Platelets
Proteomics
Stem cells
Science & Technology
title_short Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles
title_full Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles
title_fullStr Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles
title_full_unstemmed Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles
title_sort Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles
author Graça, Ana L.
author_facet Graça, Ana L.
Gomez-Florit, Manuel
Osório, Hugo
Rodrigues, Márcia T.
Domingues, Rui Miguel Andrade
Reis, R. L.
Gomes, Manuela E.
author_role author
author2 Gomez-Florit, Manuel
Osório, Hugo
Rodrigues, Márcia T.
Domingues, Rui Miguel Andrade
Reis, R. L.
Gomes, Manuela E.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Graça, Ana L.
Gomez-Florit, Manuel
Osório, Hugo
Rodrigues, Márcia T.
Domingues, Rui Miguel Andrade
Reis, R. L.
Gomes, Manuela E.
dc.subject.por.fl_str_mv Angiogenesis
Extracellular vesicles
Inflammation
Platelets
Proteomics
Stem cells
Science & Technology
topic Angiogenesis
Extracellular vesicles
Inflammation
Platelets
Proteomics
Stem cells
Science & Technology
description Extracellular vesicles (EVs) have emerged as cell-free nanotherapeutic agents for the potential treatment of multiple diseases and for tissue engineering and regenerative medicine strategies. Nevertheless, the field has typically relied on EVs derived from stem cells, the production of which in high quantities and high reproducibility is still under debate. Platelet-derived EVs were produced by a freeze–thaw method of platelet concentrates, a highly available clinical waste material. The aim of this study was to produce and thoroughly characterize platelet-derived EVs and understand their effects in adipose-tissue derived stem cells (hASCs), endothelial cells (HUVECs) and macrophages. Two different EV populations were obtained after differential centrifugation, namely small EVs (sEVs) and medium EVs (mEVs), which showed different size distributions and unique proteomic signatures. EV interaction with hASCs resulted in the modulation of the gene expression of markers related to their commitment toward different lineages. Moreover, mEVs showed higher angiogenic potential than sEVs, in a tube formation assay with HUVECs. Also, the EVs were able to modulate macrophage polarization. Altogether, these results suggest that platelet-derived EVs are promising candidates to be used as biochemical signals or therapeutic tools in tissue engineering and regenerative medicine approaches.
publishDate 2022
dc.date.none.fl_str_mv 2022-05
2022-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/80589
url https://hdl.handle.net/1822/80589
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Graça A. L., Gómez-Florit M., Osório H., Rodrigues M. T., Domingues R. M. A., Reis R. L., Gomes M. E. Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles, Nanoscale, Vol. 14, Issue 17, pp. 6543-6556, doi:10.1039/D1NR08108J, 2022
2040-3364
2040-3372
10.1039/D1NR08108J
35420605
https://pubs.rsc.org/en/content/articlelanding/2022/NR/D1NR08108J
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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