Therapeutic effects of platelet-derived extracellular vesicles in a bioengineered tendon disease model
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/79367 |
Resumo: | Tendon injuries represent over 30–50% of musculoskeletal disorders worldwide, yet the available therapies do not provide complete tendon repair/regeneration and full functionality restor-ing. Extracellular vesicles (EVs), membrane-enclosed nanoparticles, have emerged as the next breakthrough in tissue engineering and regenerative medicine to promote endogenous tissue regen-eration. Here, we developed a 3D human in vitro model mimicking the signature of pathological tendon and used it to evaluate the influence that different platelet-derived EVs might have in tendon tissue repair mechanisms. For this, different EV populations isolated from platelets, small EVs (sEVs) and medium EVs (mEVs), were added to the culture media of human tendon-derived cells (hTDCs) cultured on isotropic nanofibrous scaffolds. The platelet-derived EVs increased the expression of tenogenic markers, promoted a healthy extracellular matrix (ECM) remodeling, and the synthesis of anti-inflammatory mediators. These findings suggest that platelet EVs provided relevant biochemical cues that potentiated a recovery of hTDCs phenotype from a diseased to a healthy state. Thus, this study opens new perspectives for the translation of platelet-derived EVs as therapeutics. |
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Therapeutic effects of platelet-derived extracellular vesicles in a bioengineered tendon disease modelExtracellular vesiclesPlateletsTendinopathyTendon-derived cellsIn vitro modelsFibersTissue engineeringScience & TechnologyTendon injuries represent over 30–50% of musculoskeletal disorders worldwide, yet the available therapies do not provide complete tendon repair/regeneration and full functionality restor-ing. Extracellular vesicles (EVs), membrane-enclosed nanoparticles, have emerged as the next breakthrough in tissue engineering and regenerative medicine to promote endogenous tissue regen-eration. Here, we developed a 3D human in vitro model mimicking the signature of pathological tendon and used it to evaluate the influence that different platelet-derived EVs might have in tendon tissue repair mechanisms. For this, different EV populations isolated from platelets, small EVs (sEVs) and medium EVs (mEVs), were added to the culture media of human tendon-derived cells (hTDCs) cultured on isotropic nanofibrous scaffolds. The platelet-derived EVs increased the expression of tenogenic markers, promoted a healthy extracellular matrix (ECM) remodeling, and the synthesis of anti-inflammatory mediators. These findings suggest that platelet EVs provided relevant biochemical cues that potentiated a recovery of hTDCs phenotype from a diseased to a healthy state. Thus, this study opens new perspectives for the translation of platelet-derived EVs as therapeutics.This research was funded by the ERC CoG MagTendon grant agreement 772817; EC Twinning project Achilles 810850; Fundacao para a Ciencia e a Tecnologia (FCT) for PhD grant PD/59/2013 and PD/BD/135255/2017, individual contracts CEECIND/01375/2017 (M.G.-F.) and 2020.03410.CEECIND (R.M.A.D), and project PTDC/NAN-MAT/30595/2017.MDPIUniversidade do MinhoGraça, Ana L.Domingues, Rui Miguel AndradeCalejo, IsabelGómez-Florit, ManuelGomes, Manuela E.2022-032022-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/79367engGraça A. L., Domingues R. M. A., Calejo I., Gómez-Florit M., Gomes M. E. Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model, International Journal of Molecular Sciences, Vol. 23, Issue 6, pp. 1-16, doi:10.3390/ijms23062948, 20221661-65961422-006710.3390/ijms2306294835328370https://www.mdpi.com/1422-0067/23/6/2948info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:35Zoai:repositorium.sdum.uminho.pt:1822/79367Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:18:37.992604Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Therapeutic effects of platelet-derived extracellular vesicles in a bioengineered tendon disease model |
title |
Therapeutic effects of platelet-derived extracellular vesicles in a bioengineered tendon disease model |
spellingShingle |
Therapeutic effects of platelet-derived extracellular vesicles in a bioengineered tendon disease model Graça, Ana L. Extracellular vesicles Platelets Tendinopathy Tendon-derived cells In vitro models Fibers Tissue engineering Science & Technology |
title_short |
Therapeutic effects of platelet-derived extracellular vesicles in a bioengineered tendon disease model |
title_full |
Therapeutic effects of platelet-derived extracellular vesicles in a bioengineered tendon disease model |
title_fullStr |
Therapeutic effects of platelet-derived extracellular vesicles in a bioengineered tendon disease model |
title_full_unstemmed |
Therapeutic effects of platelet-derived extracellular vesicles in a bioengineered tendon disease model |
title_sort |
Therapeutic effects of platelet-derived extracellular vesicles in a bioengineered tendon disease model |
author |
Graça, Ana L. |
author_facet |
Graça, Ana L. Domingues, Rui Miguel Andrade Calejo, Isabel Gómez-Florit, Manuel Gomes, Manuela E. |
author_role |
author |
author2 |
Domingues, Rui Miguel Andrade Calejo, Isabel Gómez-Florit, Manuel Gomes, Manuela E. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Graça, Ana L. Domingues, Rui Miguel Andrade Calejo, Isabel Gómez-Florit, Manuel Gomes, Manuela E. |
dc.subject.por.fl_str_mv |
Extracellular vesicles Platelets Tendinopathy Tendon-derived cells In vitro models Fibers Tissue engineering Science & Technology |
topic |
Extracellular vesicles Platelets Tendinopathy Tendon-derived cells In vitro models Fibers Tissue engineering Science & Technology |
description |
Tendon injuries represent over 30–50% of musculoskeletal disorders worldwide, yet the available therapies do not provide complete tendon repair/regeneration and full functionality restor-ing. Extracellular vesicles (EVs), membrane-enclosed nanoparticles, have emerged as the next breakthrough in tissue engineering and regenerative medicine to promote endogenous tissue regen-eration. Here, we developed a 3D human in vitro model mimicking the signature of pathological tendon and used it to evaluate the influence that different platelet-derived EVs might have in tendon tissue repair mechanisms. For this, different EV populations isolated from platelets, small EVs (sEVs) and medium EVs (mEVs), were added to the culture media of human tendon-derived cells (hTDCs) cultured on isotropic nanofibrous scaffolds. The platelet-derived EVs increased the expression of tenogenic markers, promoted a healthy extracellular matrix (ECM) remodeling, and the synthesis of anti-inflammatory mediators. These findings suggest that platelet EVs provided relevant biochemical cues that potentiated a recovery of hTDCs phenotype from a diseased to a healthy state. Thus, this study opens new perspectives for the translation of platelet-derived EVs as therapeutics. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03 2022-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/79367 |
url |
https://hdl.handle.net/1822/79367 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Graça A. L., Domingues R. M. A., Calejo I., Gómez-Florit M., Gomes M. E. Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model, International Journal of Molecular Sciences, Vol. 23, Issue 6, pp. 1-16, doi:10.3390/ijms23062948, 2022 1661-6596 1422-0067 10.3390/ijms23062948 35328370 https://www.mdpi.com/1422-0067/23/6/2948 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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