Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study

Detalhes bibliográficos
Autor(a) principal: Cunha, Eva
Data de Publicação: 2021
Outros Autores: Valente, Sara, Nascimento, Mariana, Pereira, Marcelo, Tavares, Luis, Dias, Ricardo, Oliveira, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22505
Resumo: Research Areas: Science & Technology - Other Topics
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spelling Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot studyNisin-biogelOral microbiomePeriodontal diseaseDogsResearch Areas: Science & Technology - Other TopicsPeriodontal disease (PD) is one of the most widespread inflammatory diseases in dogs. This disease is initiated by a polymicrobial biofilm in the teeth surface (dental plaque), leading to a local inflammatory response, with gingivitis and/or several degrees of periodontitis. For instance, the prevention of bacterial dental plaque formation and its removal are essential steps in PD control. Recent research revealed that the antimicrobial peptide nisin incorporated in the delivery system guar gum (biogel) can inhibit and eradicate bacteria from canine dental plaque, being a promising compound for prevention of PD onset in dogs. However, no information is available regarding its effect on the dog's oral microbiome. In this pilot study, the influence of the nisin-biogel on the diversity of canine oral microbiome was evaluated using next generation sequencing (NGS), aiming to access the viability of nisin-biogel to be used in long-term experiment in dogs. Composite toothbrushing samples of the supragingival plaque from two dogs were collected at three timepoints: T1-before any application of the nisin-biogel to the animals' teeth surface; T2-one hour after one application of the nisin-biogel; and T3-one hour after a total of three applications of the nisin-biogel, each 48 hours. After that, microbial profiling was performed by NGS of the V3V4 16s rRNA region. After only one application of the nisin-biogel to the oral cavity of dogs, a statistically significant reduction in microbial diversity was observed (T2) as well as a reduction of some bacterial species potentially related with distinct stages of PD, when compared with samples collected before any application (T1). However, after a total of three nisin-biogel applications (T3), a recovery of the microbial diversity was detected. In conclusion, the nisin-biogel may influence the canine oral microbiome. A reduction in some bacterial species potentially related with distinct stages of PD was observed. This pilot study will help to design a controlled in vivo clinical trial to evaluate nisin-biogel effect on dental plaque progression and canine periodontal indices evolution in a long-term application period.PEERJ INCRepositório da Universidade de LisboaCunha, EvaValente, SaraNascimento, MarianaPereira, MarceloTavares, LuisDias, RicardoOliveira, Manuela2021-11-04T22:31:56Z2021-07-142021-07-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22505engCunha E, Valente S, Nascimento M, Pereira M, Tavares L, Dias R, Oliveira M. 2021. Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study. PeerJ 9:e11626. DOI:10.7717/peerj.116262167-835910.7717/peerj.11626info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:01Zoai:www.repository.utl.pt:10400.5/22505Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:54.943450Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study
title Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study
spellingShingle Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study
Cunha, Eva
Nisin-biogel
Oral microbiome
Periodontal disease
Dogs
title_short Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study
title_full Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study
title_fullStr Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study
title_full_unstemmed Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study
title_sort Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study
author Cunha, Eva
author_facet Cunha, Eva
Valente, Sara
Nascimento, Mariana
Pereira, Marcelo
Tavares, Luis
Dias, Ricardo
Oliveira, Manuela
author_role author
author2 Valente, Sara
Nascimento, Mariana
Pereira, Marcelo
Tavares, Luis
Dias, Ricardo
Oliveira, Manuela
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Cunha, Eva
Valente, Sara
Nascimento, Mariana
Pereira, Marcelo
Tavares, Luis
Dias, Ricardo
Oliveira, Manuela
dc.subject.por.fl_str_mv Nisin-biogel
Oral microbiome
Periodontal disease
Dogs
topic Nisin-biogel
Oral microbiome
Periodontal disease
Dogs
description Research Areas: Science & Technology - Other Topics
publishDate 2021
dc.date.none.fl_str_mv 2021-11-04T22:31:56Z
2021-07-14
2021-07-14T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22505
url http://hdl.handle.net/10400.5/22505
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cunha E, Valente S, Nascimento M, Pereira M, Tavares L, Dias R, Oliveira M. 2021. Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study. PeerJ 9:e11626. DOI:10.7717/peerj.11626
2167-8359
10.7717/peerj.11626
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PEERJ INC
publisher.none.fl_str_mv PEERJ INC
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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