Reorganisation ofHoxdregulatory landscapes during the evolution of a snake-like body plan
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/685 |
Resumo: | Within land vertebrate species, snakes display extreme variations in their body plan, characterized by the absence of limbs and an elongated morphology. Such a particular interpretation of the basic vertebrate body architecture has often been associated with changes in the function or regulation of Hox genes. Here, we use an interspecies comparative approach to investigate different regulatory aspects at the snake HoxD locus. We report that, unlike in other vertebrates, snake mesoderm-specific enhancers are mostly located within the HoxD cluster itself rather than outside. In addition, despite both the absence of limbs and an altered Hoxd gene regulation in external genitalia, the limb-associated bimodal HoxD chromatin structure is maintained at the snake locus. Finally, we show that snake and mouse orthologous enhancer sequences can display distinct expression specificities. These results show that vertebrate morphological evolution likely involved extensive reorganisation at Hox loci, yet within a generally conserved regulatory framework. |
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Reorganisation ofHoxdregulatory landscapes during the evolution of a snake-like body plandevelopmental biologyevolutionary biologygenomicsmousereptilessnakessquamatastem cellsWithin land vertebrate species, snakes display extreme variations in their body plan, characterized by the absence of limbs and an elongated morphology. Such a particular interpretation of the basic vertebrate body architecture has often been associated with changes in the function or regulation of Hox genes. Here, we use an interspecies comparative approach to investigate different regulatory aspects at the snake HoxD locus. We report that, unlike in other vertebrates, snake mesoderm-specific enhancers are mostly located within the HoxD cluster itself rather than outside. In addition, despite both the absence of limbs and an altered Hoxd gene regulation in external genitalia, the limb-associated bimodal HoxD chromatin structure is maintained at the snake locus. Finally, we show that snake and mouse orthologous enhancer sequences can display distinct expression specificities. These results show that vertebrate morphological evolution likely involved extensive reorganisation at Hox loci, yet within a generally conserved regulatory framework.Fundação para a Ciência e Tecnologia grant: (PTDB/BEX-BID/0899/2014); Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung grant: (310030B_138662); Claraz Foundation; Université de Genève; Instituto Federal de Educação Ciência e Tecnologia do Espírito Santo.eLife Sciences PublicationsARCAGuerreiro, IsabelGitto, SandraNovoa, AnaCodourey, JulienNguyen Huynh, Thi HanhGonzalez, FedericoMilinkovitch, Michel CMallo, MoisesDuboule, Denis2016-08-11T16:23:40Z2016-08-012016-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/685engeLife 2016;5:e1608710.7554/eLife.16087info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:04Zoai:arca.igc.gulbenkian.pt:10400.7/685Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:55.223953Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Reorganisation ofHoxdregulatory landscapes during the evolution of a snake-like body plan |
title |
Reorganisation ofHoxdregulatory landscapes during the evolution of a snake-like body plan |
spellingShingle |
Reorganisation ofHoxdregulatory landscapes during the evolution of a snake-like body plan Guerreiro, Isabel developmental biology evolutionary biology genomics mouse reptiles snakes squamata stem cells |
title_short |
Reorganisation ofHoxdregulatory landscapes during the evolution of a snake-like body plan |
title_full |
Reorganisation ofHoxdregulatory landscapes during the evolution of a snake-like body plan |
title_fullStr |
Reorganisation ofHoxdregulatory landscapes during the evolution of a snake-like body plan |
title_full_unstemmed |
Reorganisation ofHoxdregulatory landscapes during the evolution of a snake-like body plan |
title_sort |
Reorganisation ofHoxdregulatory landscapes during the evolution of a snake-like body plan |
author |
Guerreiro, Isabel |
author_facet |
Guerreiro, Isabel Gitto, Sandra Novoa, Ana Codourey, Julien Nguyen Huynh, Thi Hanh Gonzalez, Federico Milinkovitch, Michel C Mallo, Moises Duboule, Denis |
author_role |
author |
author2 |
Gitto, Sandra Novoa, Ana Codourey, Julien Nguyen Huynh, Thi Hanh Gonzalez, Federico Milinkovitch, Michel C Mallo, Moises Duboule, Denis |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Guerreiro, Isabel Gitto, Sandra Novoa, Ana Codourey, Julien Nguyen Huynh, Thi Hanh Gonzalez, Federico Milinkovitch, Michel C Mallo, Moises Duboule, Denis |
dc.subject.por.fl_str_mv |
developmental biology evolutionary biology genomics mouse reptiles snakes squamata stem cells |
topic |
developmental biology evolutionary biology genomics mouse reptiles snakes squamata stem cells |
description |
Within land vertebrate species, snakes display extreme variations in their body plan, characterized by the absence of limbs and an elongated morphology. Such a particular interpretation of the basic vertebrate body architecture has often been associated with changes in the function or regulation of Hox genes. Here, we use an interspecies comparative approach to investigate different regulatory aspects at the snake HoxD locus. We report that, unlike in other vertebrates, snake mesoderm-specific enhancers are mostly located within the HoxD cluster itself rather than outside. In addition, despite both the absence of limbs and an altered Hoxd gene regulation in external genitalia, the limb-associated bimodal HoxD chromatin structure is maintained at the snake locus. Finally, we show that snake and mouse orthologous enhancer sequences can display distinct expression specificities. These results show that vertebrate morphological evolution likely involved extensive reorganisation at Hox loci, yet within a generally conserved regulatory framework. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-11T16:23:40Z 2016-08-01 2016-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/685 |
url |
http://hdl.handle.net/10400.7/685 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
eLife 2016;5:e16087 10.7554/eLife.16087 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
eLife Sciences Publications |
publisher.none.fl_str_mv |
eLife Sciences Publications |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130574676295680 |