Polyhydroxyalkanoates: waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture
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Publication Date: | 2014 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/1822/32972 |
Summary: | This integrated study shows that waste glycerol can be bio-valorized by the fabrication of electrospun scaffolds for stem cells. Human mesenchymal stem cells (hMSC) provide an interesting model of regenerating cells because of their ability to differentiate into osteo-, chrondro-, adipo- and myogenic lineages. Moreover, hMSC have modulatory properties with potential on treatment of immunologic diseases. Electrospun fiber meshes offer tunable mechanical and physical properties that can mimic the structure of the native extracellular matrix, the natural environment where cells inhabit. Following a biorefinery approach, crude glycerol directly recovered from a biodiesel post-reaction stream was fed as major C source toCupriavidus necator DSM 545 to produce polyhydroxyalkanoates at polymer titers of 9â 25 g/L. Two of the P(3HB-4HB-3HV) terpolymers produced, one containing 11.4% 4HB and 3.5% 3HV and the other containing 35.6% 4HB and 3.4% 3HV, were electrospun into fibers of average diameters of 600 and 1400 nm, respectively. hMSC were cultured for 7 days in both fiber meshes, showing their ability to support stem cell growth at acceptable proliferation levels. Comparative results clearly demonstrate that scaffold topology is critical, with electrospun PHA fibers succeeding on the support of significant cell adhesion and proliferation, where planar PHA films failed. |
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Polyhydroxyalkanoates: waste glycerol upgrade into electrospun fibrous scaffolds for stem cells cultureElectrospinningMesenchymal stem cellPolyhydroxyalkanoatesStem cell scaffoldsWaste glycerolThis integrated study shows that waste glycerol can be bio-valorized by the fabrication of electrospun scaffolds for stem cells. Human mesenchymal stem cells (hMSC) provide an interesting model of regenerating cells because of their ability to differentiate into osteo-, chrondro-, adipo- and myogenic lineages. Moreover, hMSC have modulatory properties with potential on treatment of immunologic diseases. Electrospun fiber meshes offer tunable mechanical and physical properties that can mimic the structure of the native extracellular matrix, the natural environment where cells inhabit. Following a biorefinery approach, crude glycerol directly recovered from a biodiesel post-reaction stream was fed as major C source toCupriavidus necator DSM 545 to produce polyhydroxyalkanoates at polymer titers of 9â 25 g/L. Two of the P(3HB-4HB-3HV) terpolymers produced, one containing 11.4% 4HB and 3.5% 3HV and the other containing 35.6% 4HB and 3.4% 3HV, were electrospun into fibers of average diameters of 600 and 1400 nm, respectively. hMSC were cultured for 7 days in both fiber meshes, showing their ability to support stem cell growth at acceptable proliferation levels. Comparative results clearly demonstrate that scaffold topology is critical, with electrospun PHA fibers succeeding on the support of significant cell adhesion and proliferation, where planar PHA films failed.FCT -Fundação para a Ciência e a Tecnologia(SFRH/BPD/26678/2006)ElsevierUniversidade do MinhoCanadas, Raphael FaustinoCavalheiro, João M. B. T.Guerreiro, João D. T.Almeida, M. Catarina M. D. dePollet, EricSilva, Cláudia Lobato daFonseca, M. M. R. daFerreira, Frederico Castelo2014-112014-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32972engCanadas R. F., Cavalheiro J. M., Guerreiro J. D., de Almeida M. C., Pollet E., da Silva C. L., da Fonseca M. M., Ferreira F. C. Polyhydroxyalkanoates: Waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture, International Journal of Biological Macromolecules, Vol. 71, pp. 131-40, 20140141-813010.1016/j.ijbiomac.2014.05.00824836511http://www.sciencedirect.com/science/article/pii/S014181301400292Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:37:22Zoai:repositorium.sdum.uminho.pt:1822/32972Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:33:39.776954Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Polyhydroxyalkanoates: waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture |
title |
Polyhydroxyalkanoates: waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture |
spellingShingle |
Polyhydroxyalkanoates: waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture Canadas, Raphael Faustino Electrospinning Mesenchymal stem cell Polyhydroxyalkanoates Stem cell scaffolds Waste glycerol |
title_short |
Polyhydroxyalkanoates: waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture |
title_full |
Polyhydroxyalkanoates: waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture |
title_fullStr |
Polyhydroxyalkanoates: waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture |
title_full_unstemmed |
Polyhydroxyalkanoates: waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture |
title_sort |
Polyhydroxyalkanoates: waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture |
author |
Canadas, Raphael Faustino |
author_facet |
Canadas, Raphael Faustino Cavalheiro, João M. B. T. Guerreiro, João D. T. Almeida, M. Catarina M. D. de Pollet, Eric Silva, Cláudia Lobato da Fonseca, M. M. R. da Ferreira, Frederico Castelo |
author_role |
author |
author2 |
Cavalheiro, João M. B. T. Guerreiro, João D. T. Almeida, M. Catarina M. D. de Pollet, Eric Silva, Cláudia Lobato da Fonseca, M. M. R. da Ferreira, Frederico Castelo |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Canadas, Raphael Faustino Cavalheiro, João M. B. T. Guerreiro, João D. T. Almeida, M. Catarina M. D. de Pollet, Eric Silva, Cláudia Lobato da Fonseca, M. M. R. da Ferreira, Frederico Castelo |
dc.subject.por.fl_str_mv |
Electrospinning Mesenchymal stem cell Polyhydroxyalkanoates Stem cell scaffolds Waste glycerol |
topic |
Electrospinning Mesenchymal stem cell Polyhydroxyalkanoates Stem cell scaffolds Waste glycerol |
description |
This integrated study shows that waste glycerol can be bio-valorized by the fabrication of electrospun scaffolds for stem cells. Human mesenchymal stem cells (hMSC) provide an interesting model of regenerating cells because of their ability to differentiate into osteo-, chrondro-, adipo- and myogenic lineages. Moreover, hMSC have modulatory properties with potential on treatment of immunologic diseases. Electrospun fiber meshes offer tunable mechanical and physical properties that can mimic the structure of the native extracellular matrix, the natural environment where cells inhabit. Following a biorefinery approach, crude glycerol directly recovered from a biodiesel post-reaction stream was fed as major C source toCupriavidus necator DSM 545 to produce polyhydroxyalkanoates at polymer titers of 9â 25 g/L. Two of the P(3HB-4HB-3HV) terpolymers produced, one containing 11.4% 4HB and 3.5% 3HV and the other containing 35.6% 4HB and 3.4% 3HV, were electrospun into fibers of average diameters of 600 and 1400 nm, respectively. hMSC were cultured for 7 days in both fiber meshes, showing their ability to support stem cell growth at acceptable proliferation levels. Comparative results clearly demonstrate that scaffold topology is critical, with electrospun PHA fibers succeeding on the support of significant cell adhesion and proliferation, where planar PHA films failed. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-11 2014-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/32972 |
url |
http://hdl.handle.net/1822/32972 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Canadas R. F., Cavalheiro J. M., Guerreiro J. D., de Almeida M. C., Pollet E., da Silva C. L., da Fonseca M. M., Ferreira F. C. Polyhydroxyalkanoates: Waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture, International Journal of Biological Macromolecules, Vol. 71, pp. 131-40, 2014 0141-8130 10.1016/j.ijbiomac.2014.05.008 24836511 http://www.sciencedirect.com/science/article/pii/S014181301400292X |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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