Molecular diagnosis of Trichodorus and Paratrichodorus species

Detalhes bibliográficos
Autor(a) principal: Duarte, I. M.
Data de Publicação: 2004
Outros Autores: Almeida, M. T. M., Brown, D. J. F., Oliveira, C. M. G., Riga, E., Karanastasi, E., Neilson, R.
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/2364
Resumo: Some trichodorid species are known to vector specific strains of Tobacco Rattle Virus (TRV) that have a deleterious effect upon several economically important crops, e.g. potato and tobacco. Given the complex relationship that exists between trichodorids and the various TRV strains, it is imperative that a rapid and accurate identification to species level is achieved for the effective use of control measures within a crop husbandry regime. Morphological identification is sometimes subjective and requires well-trained specialists. Furthermore, there is a decline in the taxonomic skill-base of a number of invertebrate groups, including nematodes, within Europe. The present study reports the application of a PCR-RFLP method that explores the variability of the 18S rDNA gene to robustly discriminate trichodorid species belonging to Trichodorus and Paratrichodorus. A PCR product, 614 bp in length, located at the 3’ end of the 18S rDNA gene was digested with seven restriction enzymes that generated patterns that were both consistent among populations of the same species and yet discriminated trichodorids at the species level. The proposed protocol was tested with eleven trichodorid species from Portugal and six non-indigenous trichodorid species and proved to be effective.
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spelling Molecular diagnosis of Trichodorus and Paratrichodorus speciesTrichodoridsTrichodorusParatrichodorusMolecular diagnosisTRVPCR-RFLP method18S rDNAPortugalIdentificationSome trichodorid species are known to vector specific strains of Tobacco Rattle Virus (TRV) that have a deleterious effect upon several economically important crops, e.g. potato and tobacco. Given the complex relationship that exists between trichodorids and the various TRV strains, it is imperative that a rapid and accurate identification to species level is achieved for the effective use of control measures within a crop husbandry regime. Morphological identification is sometimes subjective and requires well-trained specialists. Furthermore, there is a decline in the taxonomic skill-base of a number of invertebrate groups, including nematodes, within Europe. The present study reports the application of a PCR-RFLP method that explores the variability of the 18S rDNA gene to robustly discriminate trichodorid species belonging to Trichodorus and Paratrichodorus. A PCR product, 614 bp in length, located at the 3’ end of the 18S rDNA gene was digested with seven restriction enzymes that generated patterns that were both consistent among populations of the same species and yet discriminated trichodorids at the species level. The proposed protocol was tested with eleven trichodorid species from Portugal and six non-indigenous trichodorid species and proved to be effective.Universidade do MinhoDuarte, I. M.Almeida, M. T. M.Brown, D. J. F.Oliveira, C. M. G.Riga, E.Karanastasi, E.Neilson, R.20042004-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/2364engDUARTE, I. M. [et al.] - Molecular diagnosis of Trichodorus and Paratrichodorus species. In EUROPEAN SOCIETY OF NEMATOLOGISTS ; C.N.R. INSTITUTE FOR PLANT PROTECTION. BARI, ed. lit. - “European Society of Nematologists : International Symposium, 27 : programme and abstracts” [Em linha]. [S.l. : s.n.] [c. 2004]. [Consult. 22 Jun 2005]. Disponível em: http://www.esn-symposium.ba.cnr.it/postsymposium/main.htmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:34:47Zoai:repositorium.sdum.uminho.pt:1822/2364Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:34:47Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular diagnosis of Trichodorus and Paratrichodorus species
title Molecular diagnosis of Trichodorus and Paratrichodorus species
spellingShingle Molecular diagnosis of Trichodorus and Paratrichodorus species
Duarte, I. M.
Trichodorids
Trichodorus
Paratrichodorus
Molecular diagnosis
TRV
PCR-RFLP method
18S rDNA
Portugal
Identification
title_short Molecular diagnosis of Trichodorus and Paratrichodorus species
title_full Molecular diagnosis of Trichodorus and Paratrichodorus species
title_fullStr Molecular diagnosis of Trichodorus and Paratrichodorus species
title_full_unstemmed Molecular diagnosis of Trichodorus and Paratrichodorus species
title_sort Molecular diagnosis of Trichodorus and Paratrichodorus species
author Duarte, I. M.
author_facet Duarte, I. M.
Almeida, M. T. M.
Brown, D. J. F.
Oliveira, C. M. G.
Riga, E.
Karanastasi, E.
Neilson, R.
author_role author
author2 Almeida, M. T. M.
Brown, D. J. F.
Oliveira, C. M. G.
Riga, E.
Karanastasi, E.
Neilson, R.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Duarte, I. M.
Almeida, M. T. M.
Brown, D. J. F.
Oliveira, C. M. G.
Riga, E.
Karanastasi, E.
Neilson, R.
dc.subject.por.fl_str_mv Trichodorids
Trichodorus
Paratrichodorus
Molecular diagnosis
TRV
PCR-RFLP method
18S rDNA
Portugal
Identification
topic Trichodorids
Trichodorus
Paratrichodorus
Molecular diagnosis
TRV
PCR-RFLP method
18S rDNA
Portugal
Identification
description Some trichodorid species are known to vector specific strains of Tobacco Rattle Virus (TRV) that have a deleterious effect upon several economically important crops, e.g. potato and tobacco. Given the complex relationship that exists between trichodorids and the various TRV strains, it is imperative that a rapid and accurate identification to species level is achieved for the effective use of control measures within a crop husbandry regime. Morphological identification is sometimes subjective and requires well-trained specialists. Furthermore, there is a decline in the taxonomic skill-base of a number of invertebrate groups, including nematodes, within Europe. The present study reports the application of a PCR-RFLP method that explores the variability of the 18S rDNA gene to robustly discriminate trichodorid species belonging to Trichodorus and Paratrichodorus. A PCR product, 614 bp in length, located at the 3’ end of the 18S rDNA gene was digested with seven restriction enzymes that generated patterns that were both consistent among populations of the same species and yet discriminated trichodorids at the species level. The proposed protocol was tested with eleven trichodorid species from Portugal and six non-indigenous trichodorid species and proved to be effective.
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/2364
url http://hdl.handle.net/1822/2364
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv DUARTE, I. M. [et al.] - Molecular diagnosis of Trichodorus and Paratrichodorus species. In EUROPEAN SOCIETY OF NEMATOLOGISTS ; C.N.R. INSTITUTE FOR PLANT PROTECTION. BARI, ed. lit. - “European Society of Nematologists : International Symposium, 27 : programme and abstracts” [Em linha]. [S.l. : s.n.] [c. 2004]. [Consult. 22 Jun 2005]. Disponível em: http://www.esn-symposium.ba.cnr.it/postsymposium/main.html
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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