Molecular diagnosis of Trichodorus and Paratrichodorus species
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/2364 |
Resumo: | Some trichodorid species are known to vector specific strains of Tobacco Rattle Virus (TRV) that have a deleterious effect upon several economically important crops, e.g. potato and tobacco. Given the complex relationship that exists between trichodorids and the various TRV strains, it is imperative that a rapid and accurate identification to species level is achieved for the effective use of control measures within a crop husbandry regime. Morphological identification is sometimes subjective and requires well-trained specialists. Furthermore, there is a decline in the taxonomic skill-base of a number of invertebrate groups, including nematodes, within Europe. The present study reports the application of a PCR-RFLP method that explores the variability of the 18S rDNA gene to robustly discriminate trichodorid species belonging to Trichodorus and Paratrichodorus. A PCR product, 614 bp in length, located at the 3’ end of the 18S rDNA gene was digested with seven restriction enzymes that generated patterns that were both consistent among populations of the same species and yet discriminated trichodorids at the species level. The proposed protocol was tested with eleven trichodorid species from Portugal and six non-indigenous trichodorid species and proved to be effective. |
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Molecular diagnosis of Trichodorus and Paratrichodorus speciesTrichodoridsTrichodorusParatrichodorusMolecular diagnosisTRVPCR-RFLP method18S rDNAPortugalIdentificationSome trichodorid species are known to vector specific strains of Tobacco Rattle Virus (TRV) that have a deleterious effect upon several economically important crops, e.g. potato and tobacco. Given the complex relationship that exists between trichodorids and the various TRV strains, it is imperative that a rapid and accurate identification to species level is achieved for the effective use of control measures within a crop husbandry regime. Morphological identification is sometimes subjective and requires well-trained specialists. Furthermore, there is a decline in the taxonomic skill-base of a number of invertebrate groups, including nematodes, within Europe. The present study reports the application of a PCR-RFLP method that explores the variability of the 18S rDNA gene to robustly discriminate trichodorid species belonging to Trichodorus and Paratrichodorus. A PCR product, 614 bp in length, located at the 3’ end of the 18S rDNA gene was digested with seven restriction enzymes that generated patterns that were both consistent among populations of the same species and yet discriminated trichodorids at the species level. The proposed protocol was tested with eleven trichodorid species from Portugal and six non-indigenous trichodorid species and proved to be effective.Universidade do MinhoDuarte, I. M.Almeida, M. T. M.Brown, D. J. F.Oliveira, C. M. G.Riga, E.Karanastasi, E.Neilson, R.20042004-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/2364engDUARTE, I. M. [et al.] - Molecular diagnosis of Trichodorus and Paratrichodorus species. In EUROPEAN SOCIETY OF NEMATOLOGISTS ; C.N.R. INSTITUTE FOR PLANT PROTECTION. BARI, ed. lit. - “European Society of Nematologists : International Symposium, 27 : programme and abstracts” [Em linha]. [S.l. : s.n.] [c. 2004]. [Consult. 22 Jun 2005]. Disponível em: http://www.esn-symposium.ba.cnr.it/postsymposium/main.htmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:34:47Zoai:repositorium.sdum.uminho.pt:1822/2364Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:34:47Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Molecular diagnosis of Trichodorus and Paratrichodorus species |
title |
Molecular diagnosis of Trichodorus and Paratrichodorus species |
spellingShingle |
Molecular diagnosis of Trichodorus and Paratrichodorus species Duarte, I. M. Trichodorids Trichodorus Paratrichodorus Molecular diagnosis TRV PCR-RFLP method 18S rDNA Portugal Identification |
title_short |
Molecular diagnosis of Trichodorus and Paratrichodorus species |
title_full |
Molecular diagnosis of Trichodorus and Paratrichodorus species |
title_fullStr |
Molecular diagnosis of Trichodorus and Paratrichodorus species |
title_full_unstemmed |
Molecular diagnosis of Trichodorus and Paratrichodorus species |
title_sort |
Molecular diagnosis of Trichodorus and Paratrichodorus species |
author |
Duarte, I. M. |
author_facet |
Duarte, I. M. Almeida, M. T. M. Brown, D. J. F. Oliveira, C. M. G. Riga, E. Karanastasi, E. Neilson, R. |
author_role |
author |
author2 |
Almeida, M. T. M. Brown, D. J. F. Oliveira, C. M. G. Riga, E. Karanastasi, E. Neilson, R. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Duarte, I. M. Almeida, M. T. M. Brown, D. J. F. Oliveira, C. M. G. Riga, E. Karanastasi, E. Neilson, R. |
dc.subject.por.fl_str_mv |
Trichodorids Trichodorus Paratrichodorus Molecular diagnosis TRV PCR-RFLP method 18S rDNA Portugal Identification |
topic |
Trichodorids Trichodorus Paratrichodorus Molecular diagnosis TRV PCR-RFLP method 18S rDNA Portugal Identification |
description |
Some trichodorid species are known to vector specific strains of Tobacco Rattle Virus (TRV) that have a deleterious effect upon several economically important crops, e.g. potato and tobacco. Given the complex relationship that exists between trichodorids and the various TRV strains, it is imperative that a rapid and accurate identification to species level is achieved for the effective use of control measures within a crop husbandry regime. Morphological identification is sometimes subjective and requires well-trained specialists. Furthermore, there is a decline in the taxonomic skill-base of a number of invertebrate groups, including nematodes, within Europe. The present study reports the application of a PCR-RFLP method that explores the variability of the 18S rDNA gene to robustly discriminate trichodorid species belonging to Trichodorus and Paratrichodorus. A PCR product, 614 bp in length, located at the 3’ end of the 18S rDNA gene was digested with seven restriction enzymes that generated patterns that were both consistent among populations of the same species and yet discriminated trichodorids at the species level. The proposed protocol was tested with eleven trichodorid species from Portugal and six non-indigenous trichodorid species and proved to be effective. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 2004-01-01T00:00:00Z |
dc.type.driver.fl_str_mv |
conference object |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/2364 |
url |
http://hdl.handle.net/1822/2364 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
DUARTE, I. M. [et al.] - Molecular diagnosis of Trichodorus and Paratrichodorus species. In EUROPEAN SOCIETY OF NEMATOLOGISTS ; C.N.R. INSTITUTE FOR PLANT PROTECTION. BARI, ed. lit. - “European Society of Nematologists : International Symposium, 27 : programme and abstracts” [Em linha]. [S.l. : s.n.] [c. 2004]. [Consult. 22 Jun 2005]. Disponível em: http://www.esn-symposium.ba.cnr.it/postsymposium/main.html |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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