MicroRNA processing machinery in the developing chick embryo

Detalhes bibliográficos
Autor(a) principal: Carraco, Gil
Data de Publicação: 2014
Outros Autores: Gonçalves, Ana N., Serra, Carlos, Andrade, Raquel P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/32919
Resumo: Uncorrected proof
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spelling MicroRNA processing machinery in the developing chick embryoDROSHADGCR8XPO5DICER1Chick embryoMicroRNA machineryScience & TechnologyUncorrected proofGene expression regulation during embryo development is under strict regulation to ensure proper gene expression in both time and space. The involvement of microRNAs (miRNA) in early vertebrate development is documented and inactivation of different proteins involved in miRNA synthesis results in severe malformations or even arrests vertebrate embryo development. However, there is very limited information on when and in what tissues the genes encoding these proteins are expressed. Herein, we report a detailed characterization of the expression patterns of DROSHA, DGCR8, XPO5 and DICER1 in the developing chick embryo, from HH1 (when the egg is laid) to HH25 (5-days incubation), using whole mount in situ hybridization and cross-section analysis. We found that these genes are co-expressed in multiple tissues, mostly after stage HH4. Before early gastrulation DICER1 expression was never detected, suggesting the operation of a Dicer-independent pathway for miRNA synthesis. Our results support an important role for miRNAs in vertebrate embryo development and provide the necessary framework to unveil additional roles for these RNA processing proteins in development.The authors thank C.J. Sheeba for lab coaching to C.S. and I. Palmeirim, S. Thorsteinsdottir and lab members for helpful discussions. G.C. and A.C.G. were supported by Fundacao para a Ciencia e a Tecnologia (FCT), Portugal (grants UMINHO/BI/282/2013 and UMINHO/BI/8/2014, respectively). R.P.A. was funded by Programa Operacional Regional do Norte (ON.2) NORTE-07-0124-FEDER-ElsevierUniversidade do MinhoCarraco, GilGonçalves, Ana N.Serra, CarlosAndrade, Raquel P.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32919eng1567-133X10.1016/j.gep.2014.09.00225266146http://www.sciencedirect.com/science/article/pii/S1567133X14000763info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:46:59Zoai:repositorium.sdum.uminho.pt:1822/32919Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:45:02.176325Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv MicroRNA processing machinery in the developing chick embryo
title MicroRNA processing machinery in the developing chick embryo
spellingShingle MicroRNA processing machinery in the developing chick embryo
Carraco, Gil
DROSHA
DGCR8
XPO5
DICER1
Chick embryo
MicroRNA machinery
Science & Technology
title_short MicroRNA processing machinery in the developing chick embryo
title_full MicroRNA processing machinery in the developing chick embryo
title_fullStr MicroRNA processing machinery in the developing chick embryo
title_full_unstemmed MicroRNA processing machinery in the developing chick embryo
title_sort MicroRNA processing machinery in the developing chick embryo
author Carraco, Gil
author_facet Carraco, Gil
Gonçalves, Ana N.
Serra, Carlos
Andrade, Raquel P.
author_role author
author2 Gonçalves, Ana N.
Serra, Carlos
Andrade, Raquel P.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Carraco, Gil
Gonçalves, Ana N.
Serra, Carlos
Andrade, Raquel P.
dc.subject.por.fl_str_mv DROSHA
DGCR8
XPO5
DICER1
Chick embryo
MicroRNA machinery
Science & Technology
topic DROSHA
DGCR8
XPO5
DICER1
Chick embryo
MicroRNA machinery
Science & Technology
description Uncorrected proof
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/32919
url http://hdl.handle.net/1822/32919
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1567-133X
10.1016/j.gep.2014.09.002
25266146
http://www.sciencedirect.com/science/article/pii/S1567133X14000763
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799133012984594432

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