Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus

Detalhes bibliográficos
Autor(a) principal: Santos, Paloma Daguer Ewerton dos
Data de Publicação: 2021
Outros Autores: Melo, Karla Fabiane Lopes de, Franco Neto, Walter Felix, Casseb, Samir Mansour Moraes, Cruz, Ana Cecília Ribeiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/12035
Resumo: Introduction: The Mayaro virus (MAYV) is an arbovirus belonging to the Togaviridae family of the alphavirus genus, which is transmitted by hematophagous arthropods to vertebrate hosts, whereas non-human primates are considered primary reservoirs. Objective: To evaluate the expression of messenger RNAs (mRNA) of crucial proteins in the microRNA pathway and inflammatory cytokines in human cells during infection by the MAYV. Materials and Methods: Viral samples propagated in RD cell cultures. The RNAs were collected and extracted at predetermined times. The total RNA was then used to detect mRNA by PCR in real time (qRT-PCR). Results: the Mayaro virus RNA was verified in the cells during all the days of infection. The AgR2 mRNA protein dropped sharply by 2 dpi and 3 dpi. Conclusion: The present study demonstrated the reduction of proteins in infection with the Mayaro virus.
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spelling Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus Evaluación de la expresión de RNA mensajero (RNAm) de las proteínas clave de la ruta del microRNA (miRNA) en células RD infectadas con el Virus MayaroAvaliação da expressão de RNA mensageiro (RNAm) das proteínas-chave da via do microRNA (miRNA) em células RD infectadas com o vírus MayaroMayaroMicroRNADicerDrosha.MayaroMicroRNADicerDrosha.MayaroMicroRNADicerDrosha.Introduction: The Mayaro virus (MAYV) is an arbovirus belonging to the Togaviridae family of the alphavirus genus, which is transmitted by hematophagous arthropods to vertebrate hosts, whereas non-human primates are considered primary reservoirs. Objective: To evaluate the expression of messenger RNAs (mRNA) of crucial proteins in the microRNA pathway and inflammatory cytokines in human cells during infection by the MAYV. Materials and Methods: Viral samples propagated in RD cell cultures. The RNAs were collected and extracted at predetermined times. The total RNA was then used to detect mRNA by PCR in real time (qRT-PCR). Results: the Mayaro virus RNA was verified in the cells during all the days of infection. The AgR2 mRNA protein dropped sharply by 2 dpi and 3 dpi. Conclusion: The present study demonstrated the reduction of proteins in infection with the Mayaro virus.Introducción: El virus Mayaro (MAYV) es un arbovirus perteneciente a la familia Togaviridae del género alfavirus, que se transmite por artrópodos hematófagos a hospederos vertebrados, mientras que los primates no humanos se consideran reservorios primarios. Objetivo: Evaluar la expresión de RNA mensajeros (RNAm) de proteínas cruciales en la vía del microARN y citocinas inflamatorias en células humanas durante la infección por el virus MAYV. Materiales y métodos: Muestras virales propagadas en cultivos de células RD. Los ARN se recogieron y extrajeron en momentos predeterminados. El ARN total se utilizó luego para detectar RNAm por PCR en tiempo real (qRT-PCR). Resultados: se verificó el RNA del virus Mayaro en las células durante todos los días de infección. La proteína de ARNm de AgR 2 se redujo drásticamente en 2 dpi y 3 dpi. Conclusión: El presente estudio demostró la reducción de proteínas en la infección por el virus Mayaro.Introdução: O vírus Mayaro (MAYV) é um arbovírus pertencente à família Togaviridae do gênero alfavírus, que é transmitido por artrópodes hematófagos a hospedeiros vertebrados, enquanto primatas não humanos são considerados reservatórios primários. Objetivo: Avaliar a expressão de RNAs mensageiros (mRNA) de proteínas cruciais na via de microRNAs e citocinas inflamatórias em células humanas durante a infecção pelo vírus MAYV. Materiais e Métodos: Amostras virais propagadas em culturas de células RD. Os RNAs foram coletados e extraídos em horários predeterminados. O RNA total foi então usado para detectar mRNA por PCR em tempo real (qRT-PCR). Resultados: o RNA do vírus Mayaro foi verificado nas células durante todos os dias de infecção. A proteína AgR2 mRNA caiu drasticamente em 2 dpi e 3 dpi. Conclusão: O presente estudo demonstrou redução de proteínas na infecção pelo vírus Mayaro.Research, Society and Development2021-01-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1203510.33448/rsd-v10i1.12035Research, Society and Development; Vol. 10 No. 1; e55610112035Research, Society and Development; Vol. 10 Núm. 1; e55610112035Research, Society and Development; v. 10 n. 1; e556101120352525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/12035/10860Copyright (c) 2021 Paloma Daguer Ewerton dos Santos; Karla Fabiane Lopes de Melo; Walter Felix Franco Neto; Samir Mansour Moraes Casseb; Ana Cecília Ribeiro Cruzhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSantos, Paloma Daguer Ewerton dosMelo, Karla Fabiane Lopes deFranco Neto, Walter Felix Casseb, Samir Mansour MoraesCruz, Ana Cecília Ribeiro2021-02-20T21:19:23Zoai:ojs.pkp.sfu.ca:article/12035Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:43.239910Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus
Evaluación de la expresión de RNA mensajero (RNAm) de las proteínas clave de la ruta del microRNA (miRNA) en células RD infectadas con el Virus Mayaro
Avaliação da expressão de RNA mensageiro (RNAm) das proteínas-chave da via do microRNA (miRNA) em células RD infectadas com o vírus Mayaro
title Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus
spellingShingle Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus
Santos, Paloma Daguer Ewerton dos
Mayaro
MicroRNA
Dicer
Drosha.
Mayaro
MicroRNA
Dicer
Drosha.
Mayaro
MicroRNA
Dicer
Drosha.
title_short Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus
title_full Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus
title_fullStr Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus
title_full_unstemmed Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus
title_sort Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus
author Santos, Paloma Daguer Ewerton dos
author_facet Santos, Paloma Daguer Ewerton dos
Melo, Karla Fabiane Lopes de
Franco Neto, Walter Felix
Casseb, Samir Mansour Moraes
Cruz, Ana Cecília Ribeiro
author_role author
author2 Melo, Karla Fabiane Lopes de
Franco Neto, Walter Felix
Casseb, Samir Mansour Moraes
Cruz, Ana Cecília Ribeiro
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santos, Paloma Daguer Ewerton dos
Melo, Karla Fabiane Lopes de
Franco Neto, Walter Felix
Casseb, Samir Mansour Moraes
Cruz, Ana Cecília Ribeiro
dc.subject.por.fl_str_mv Mayaro
MicroRNA
Dicer
Drosha.
Mayaro
MicroRNA
Dicer
Drosha.
Mayaro
MicroRNA
Dicer
Drosha.
topic Mayaro
MicroRNA
Dicer
Drosha.
Mayaro
MicroRNA
Dicer
Drosha.
Mayaro
MicroRNA
Dicer
Drosha.
description Introduction: The Mayaro virus (MAYV) is an arbovirus belonging to the Togaviridae family of the alphavirus genus, which is transmitted by hematophagous arthropods to vertebrate hosts, whereas non-human primates are considered primary reservoirs. Objective: To evaluate the expression of messenger RNAs (mRNA) of crucial proteins in the microRNA pathway and inflammatory cytokines in human cells during infection by the MAYV. Materials and Methods: Viral samples propagated in RD cell cultures. The RNAs were collected and extracted at predetermined times. The total RNA was then used to detect mRNA by PCR in real time (qRT-PCR). Results: the Mayaro virus RNA was verified in the cells during all the days of infection. The AgR2 mRNA protein dropped sharply by 2 dpi and 3 dpi. Conclusion: The present study demonstrated the reduction of proteins in infection with the Mayaro virus.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/12035
10.33448/rsd-v10i1.12035
url https://rsdjournal.org/index.php/rsd/article/view/12035
identifier_str_mv 10.33448/rsd-v10i1.12035
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/12035/10860
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 1; e55610112035
Research, Society and Development; Vol. 10 Núm. 1; e55610112035
Research, Society and Development; v. 10 n. 1; e55610112035
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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