Assessment of yeast viability under hyperbaric conditions through a modeling approach

Detalhes bibliográficos
Autor(a) principal: Coutinho, J. A. P.
Data de Publicação: 2005
Outros Autores: Belo, Isabel, Ferreira, Eugénio C., Coelho, M. A. Z.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/3368
Resumo: The effect of pressure (0.1–1.5 MPa) and oxygen concentration on the growth and viability of Saccharomyces cerevisiae was studied. Cell viability was assessed through the Methylene Blue staining method and the percentages of viable and non-viable cells were estimated using digital image processing. A model taking into account cell viability was developed and used to describe the measured data. The model reveals the opposing effects between oxygen availability and the baric and oxidative stresses present on the system and can successfully describe not only the traditional biomass–product–substrate (X–P–S) volution but also the ratio of viable cells with time. It is shown that cell viability in general is not constant during the experiments but strongly depends on the environment.
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spelling Assessment of yeast viability under hyperbaric conditions through a modeling approachModelingDigital image processingCell viabilityYeastScience & TechnologyThe effect of pressure (0.1–1.5 MPa) and oxygen concentration on the growth and viability of Saccharomyces cerevisiae was studied. Cell viability was assessed through the Methylene Blue staining method and the percentages of viable and non-viable cells were estimated using digital image processing. A model taking into account cell viability was developed and used to describe the measured data. The model reveals the opposing effects between oxygen availability and the baric and oxidative stresses present on the system and can successfully describe not only the traditional biomass–product–substrate (X–P–S) volution but also the ratio of viable cells with time. It is shown that cell viability in general is not constant during the experiments but strongly depends on the environment.WileyUniversidade do MinhoCoutinho, J. A. P.Belo, IsabelFerreira, Eugénio C.Coelho, M. A. Z.2005-082005-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/3368eng"Journal of Chemical Tecnology and Biotechnology". ISSN 0268-2575. 80:8 (Aug. 2005) 872-877.0268-257510.1002/jctb.1248http://www3.interscience.wiley.com/cgi-bin/fulltext/110476309/PDFSTARTinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:20:36Zoai:repositorium.sdum.uminho.pt:1822/3368Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:13:47.629450Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Assessment of yeast viability under hyperbaric conditions through a modeling approach
title Assessment of yeast viability under hyperbaric conditions through a modeling approach
spellingShingle Assessment of yeast viability under hyperbaric conditions through a modeling approach
Coutinho, J. A. P.
Modeling
Digital image processing
Cell viability
Yeast
Science & Technology
title_short Assessment of yeast viability under hyperbaric conditions through a modeling approach
title_full Assessment of yeast viability under hyperbaric conditions through a modeling approach
title_fullStr Assessment of yeast viability under hyperbaric conditions through a modeling approach
title_full_unstemmed Assessment of yeast viability under hyperbaric conditions through a modeling approach
title_sort Assessment of yeast viability under hyperbaric conditions through a modeling approach
author Coutinho, J. A. P.
author_facet Coutinho, J. A. P.
Belo, Isabel
Ferreira, Eugénio C.
Coelho, M. A. Z.
author_role author
author2 Belo, Isabel
Ferreira, Eugénio C.
Coelho, M. A. Z.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Coutinho, J. A. P.
Belo, Isabel
Ferreira, Eugénio C.
Coelho, M. A. Z.
dc.subject.por.fl_str_mv Modeling
Digital image processing
Cell viability
Yeast
Science & Technology
topic Modeling
Digital image processing
Cell viability
Yeast
Science & Technology
description The effect of pressure (0.1–1.5 MPa) and oxygen concentration on the growth and viability of Saccharomyces cerevisiae was studied. Cell viability was assessed through the Methylene Blue staining method and the percentages of viable and non-viable cells were estimated using digital image processing. A model taking into account cell viability was developed and used to describe the measured data. The model reveals the opposing effects between oxygen availability and the baric and oxidative stresses present on the system and can successfully describe not only the traditional biomass–product–substrate (X–P–S) volution but also the ratio of viable cells with time. It is shown that cell viability in general is not constant during the experiments but strongly depends on the environment.
publishDate 2005
dc.date.none.fl_str_mv 2005-08
2005-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/3368
url https://hdl.handle.net/1822/3368
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of Chemical Tecnology and Biotechnology". ISSN 0268-2575. 80:8 (Aug. 2005) 872-877.
0268-2575
10.1002/jctb.1248
http://www3.interscience.wiley.com/cgi-bin/fulltext/110476309/PDFSTART
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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