Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/3037 |
Resumo: | The in vitro antioxidant and growth inhibitory activity of extracts obtained from two Portuguese wild mushrooms (Clitocybe alexandri and Lepista inversa) was studied in human tumour cell lines. The extracts were phenolic (methanolic and ethanolic) and polysaccharidic (boiling water). The antioxidant activity assays included evaluation of radical-scavenging capacity, reducing power and inhibition of lipid peroxidation measured in liposome solutions. Extract-induced cell growth inhibition was measured in four different tumour cell lines (lung, breast, colon and gastric cancer) using the SRB assay. The polysaccharidic extract oft. inversa was the most potent as antioxidant (EC50 < 1.8 +/- 0.1 mg/ml), while the phenolic ethanolic extract of C alexandri was the most potent as inhibitor of growth of the studied cancer cell lines (Gl(50) <26.0 +/- 1.3 mu g/ml). Together, these activities indicate that these mushrooms are promising sources of bioactive compounds. (C) 2010 Elsevier Ltd. All rights reserved. |
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Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell linesWild mushroomsAntioxidant activityGrowth inhibitory activityThe in vitro antioxidant and growth inhibitory activity of extracts obtained from two Portuguese wild mushrooms (Clitocybe alexandri and Lepista inversa) was studied in human tumour cell lines. The extracts were phenolic (methanolic and ethanolic) and polysaccharidic (boiling water). The antioxidant activity assays included evaluation of radical-scavenging capacity, reducing power and inhibition of lipid peroxidation measured in liposome solutions. Extract-induced cell growth inhibition was measured in four different tumour cell lines (lung, breast, colon and gastric cancer) using the SRB assay. The polysaccharidic extract oft. inversa was the most potent as antioxidant (EC50 < 1.8 +/- 0.1 mg/ml), while the phenolic ethanolic extract of C alexandri was the most potent as inhibitor of growth of the studied cancer cell lines (Gl(50) <26.0 +/- 1.3 mu g/ml). Together, these activities indicate that these mushrooms are promising sources of bioactive compounds. (C) 2010 Elsevier Ltd. All rights reserved.ElsevierBiblioteca Digital do IPBVaz, Josiana A.Heleno, Sandrina A.Martins, AnabelaAlmeida, Gabriela M.Vasconcelos, M. HelenaFerreira, Isabel C.F.R.2011-01-06T16:54:03Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10198/3037engVaz, Josiana A.; Heleno, Sandrina A.; Martins, Anabela; Almeida, Gabriela M.; Vasconcelos, M. Helena; Ferreira, Isabel C.F.R. (2010). Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines. Food and Chemical Toxicology. ISSN 0278-6915. 48:10, p. 2881-28840278-691510.1016/j.fct.2010.07.021info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:07:57Zoai:bibliotecadigital.ipb.pt:10198/3037Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:55:58.713387Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines |
title |
Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines |
spellingShingle |
Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines Vaz, Josiana A. Wild mushrooms Antioxidant activity Growth inhibitory activity |
title_short |
Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines |
title_full |
Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines |
title_fullStr |
Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines |
title_full_unstemmed |
Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines |
title_sort |
Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines |
author |
Vaz, Josiana A. |
author_facet |
Vaz, Josiana A. Heleno, Sandrina A. Martins, Anabela Almeida, Gabriela M. Vasconcelos, M. Helena Ferreira, Isabel C.F.R. |
author_role |
author |
author2 |
Heleno, Sandrina A. Martins, Anabela Almeida, Gabriela M. Vasconcelos, M. Helena Ferreira, Isabel C.F.R. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Vaz, Josiana A. Heleno, Sandrina A. Martins, Anabela Almeida, Gabriela M. Vasconcelos, M. Helena Ferreira, Isabel C.F.R. |
dc.subject.por.fl_str_mv |
Wild mushrooms Antioxidant activity Growth inhibitory activity |
topic |
Wild mushrooms Antioxidant activity Growth inhibitory activity |
description |
The in vitro antioxidant and growth inhibitory activity of extracts obtained from two Portuguese wild mushrooms (Clitocybe alexandri and Lepista inversa) was studied in human tumour cell lines. The extracts were phenolic (methanolic and ethanolic) and polysaccharidic (boiling water). The antioxidant activity assays included evaluation of radical-scavenging capacity, reducing power and inhibition of lipid peroxidation measured in liposome solutions. Extract-induced cell growth inhibition was measured in four different tumour cell lines (lung, breast, colon and gastric cancer) using the SRB assay. The polysaccharidic extract oft. inversa was the most potent as antioxidant (EC50 < 1.8 +/- 0.1 mg/ml), while the phenolic ethanolic extract of C alexandri was the most potent as inhibitor of growth of the studied cancer cell lines (Gl(50) <26.0 +/- 1.3 mu g/ml). Together, these activities indicate that these mushrooms are promising sources of bioactive compounds. (C) 2010 Elsevier Ltd. All rights reserved. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z 2011-01-06T16:54:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/3037 |
url |
http://hdl.handle.net/10198/3037 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vaz, Josiana A.; Heleno, Sandrina A.; Martins, Anabela; Almeida, Gabriela M.; Vasconcelos, M. Helena; Ferreira, Isabel C.F.R. (2010). Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines. Food and Chemical Toxicology. ISSN 0278-6915. 48:10, p. 2881-2884 0278-6915 10.1016/j.fct.2010.07.021 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799135165951246336 |