Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human blood
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.26/36706 |
Resumo: | This paper presents a method for the determination of xylazine in whole blood using solid-phase extraction and gas chromatography-mass spectrometry. This technique required only 0.5 mL of sample, and protriptyline was used as internal standard (IS). Limits of detection and quantitation (LOQ) were 2 and 10 ng/mL, respectively. The method was found to be linear between the LOQ and 3.50 microg/mL, with correlation coefficients higher than 0.9922. Precision (intra- and interday) and accuracy were in conformity with the criteria normally accepted in bioanalytical method validation. The analyte was stable in the matrix for at least 18 h at room temperature and for at least three freeze/thaw cycles. Mean recovery, calculated at three concentration levels, was 87%. To the best of our knowledge, this is the first time that solid-phase extraction is used as sample preparation technique for the determination of this compound in biological media. Because of its simplicity and speed when compared to other extraction techniques, the herein described method can be successfully applied in the diagnosis of intoxications by xylazine. |
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Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human bloodDrug StabilityHumansReproducibility of ResultsToxicologyVeterinary DrugsXylazineGas Chromatography-Mass SpectrometrySolid Phase ExtractionThis paper presents a method for the determination of xylazine in whole blood using solid-phase extraction and gas chromatography-mass spectrometry. This technique required only 0.5 mL of sample, and protriptyline was used as internal standard (IS). Limits of detection and quantitation (LOQ) were 2 and 10 ng/mL, respectively. The method was found to be linear between the LOQ and 3.50 microg/mL, with correlation coefficients higher than 0.9922. Precision (intra- and interday) and accuracy were in conformity with the criteria normally accepted in bioanalytical method validation. The analyte was stable in the matrix for at least 18 h at room temperature and for at least three freeze/thaw cycles. Mean recovery, calculated at three concentration levels, was 87%. To the best of our knowledge, this is the first time that solid-phase extraction is used as sample preparation technique for the determination of this compound in biological media. Because of its simplicity and speed when compared to other extraction techniques, the herein described method can be successfully applied in the diagnosis of intoxications by xylazine.info:eu-repo/semantics/publishedVersionOxford University Press2021-06-07T15:21:41Z2021-06-072007-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10400.26/36706oai:comum.rcaap.pt:10400.26/36706engJournal of Analytical Toxicology 2007; 31(3): 165-91945-2403http://hdl.handle.net/10400.26/36706https://doi.org/10.1093/jat/31.3.165https://academic.oup.com/jat/article/31/3/165/712199Barroso, MárioGallardo, EugeniaMargalho, CláudiaDevesa, NPimentel, JVieira, Duarte Nunoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-20T14:25:34Zoai:comum.rcaap.pt:10400.26/36706Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:23:04.766128Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human blood |
title |
Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human blood |
spellingShingle |
Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human blood Barroso, Mário Drug Stability Humans Reproducibility of Results Toxicology Veterinary Drugs Xylazine Gas Chromatography-Mass Spectrometry Solid Phase Extraction |
title_short |
Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human blood |
title_full |
Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human blood |
title_fullStr |
Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human blood |
title_full_unstemmed |
Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human blood |
title_sort |
Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human blood |
author |
Barroso, Mário |
author_facet |
Barroso, Mário Gallardo, Eugenia Margalho, Cláudia Devesa, N Pimentel, J Vieira, Duarte Nuno |
author_role |
author |
author2 |
Gallardo, Eugenia Margalho, Cláudia Devesa, N Pimentel, J Vieira, Duarte Nuno |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Barroso, Mário Gallardo, Eugenia Margalho, Cláudia Devesa, N Pimentel, J Vieira, Duarte Nuno |
dc.subject.por.fl_str_mv |
Drug Stability Humans Reproducibility of Results Toxicology Veterinary Drugs Xylazine Gas Chromatography-Mass Spectrometry Solid Phase Extraction |
topic |
Drug Stability Humans Reproducibility of Results Toxicology Veterinary Drugs Xylazine Gas Chromatography-Mass Spectrometry Solid Phase Extraction |
description |
This paper presents a method for the determination of xylazine in whole blood using solid-phase extraction and gas chromatography-mass spectrometry. This technique required only 0.5 mL of sample, and protriptyline was used as internal standard (IS). Limits of detection and quantitation (LOQ) were 2 and 10 ng/mL, respectively. The method was found to be linear between the LOQ and 3.50 microg/mL, with correlation coefficients higher than 0.9922. Precision (intra- and interday) and accuracy were in conformity with the criteria normally accepted in bioanalytical method validation. The analyte was stable in the matrix for at least 18 h at room temperature and for at least three freeze/thaw cycles. Mean recovery, calculated at three concentration levels, was 87%. To the best of our knowledge, this is the first time that solid-phase extraction is used as sample preparation technique for the determination of this compound in biological media. Because of its simplicity and speed when compared to other extraction techniques, the herein described method can be successfully applied in the diagnosis of intoxications by xylazine. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-04-01T00:00:00Z 2021-06-07T15:21:41Z 2021-06-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.26/36706 oai:comum.rcaap.pt:10400.26/36706 |
url |
http://hdl.handle.net/10400.26/36706 |
identifier_str_mv |
oai:comum.rcaap.pt:10400.26/36706 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Analytical Toxicology 2007; 31(3): 165-9 1945-2403 http://hdl.handle.net/10400.26/36706 https://doi.org/10.1093/jat/31.3.165 https://academic.oup.com/jat/article/31/3/165/712199 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130675957202944 |