Immunohistochemistry detection of putative miR-200c and miR-203 targets in breast cancer patients

Detalhes bibliográficos
Autor(a) principal: Santos, Bruno Filipe da Silva
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/13914
Resumo: Part of this thesis will be published in the following: Gomes, B.C., Santos, B. 2015. Methods for studying microRNAs expression and their targets in formalin-fixed, paraffin-embedded (FFPE) breast cancer tissues. In Methods in Molecular Biology: Cancer Drug Resistance (Rueff, J. & Rodrigues, A.S. eds), Springer Protocols.
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spelling Immunohistochemistry detection of putative miR-200c and miR-203 targets in breast cancer patientsATMBMI1Breast cancermiR-200cmiR-203SIX1Part of this thesis will be published in the following: Gomes, B.C., Santos, B. 2015. Methods for studying microRNAs expression and their targets in formalin-fixed, paraffin-embedded (FFPE) breast cancer tissues. In Methods in Molecular Biology: Cancer Drug Resistance (Rueff, J. & Rodrigues, A.S. eds), Springer Protocols.The morphological variability and clinical evolution of breast cancer have prompted researchers to find a strategy to classify the disease and to possibly define supportive prognostic and predictive indicators. Recently, some studies have focused on the putative utility of miRNA as a novel class of cancer markers. Since potential targets of miRNAs are often provided only by bioinformatic tools there is a gap in this area of study. Therefore, parallel to the quantification of the expression of miR-203 and miR-200c in tumor tissue from patients from Central Lisbon Hospital with breast cancer, the aim of this dissertation was to analyze the expression of their putative targets – ATM, BMI1, SIX1 and SOX2 – by immunohistochemistry. 45 samples were analyzed corresponding to 43 patients whose mean age at diagnosis was 62 years. The most common type was invasive carcinoma NOS (71,1%) followed by invasive lobular carcinoma (8,9%). 86,4% of samples were ER positive, 79,1% PR positive, 13,6% HER2 positive and 45,5% high ki67. miR-200c was downregulated in 12,8% of the samples and upregulated in 23,1%. Comparatively, 20,5% of tumors presented miR-203 downregulation and 30,8% upregulation. Anti-ATM and anti-BMI1 antibodies didn’t perform properly thus, they were not assessed. Regarding to SIX1 and SOX2, only 13.3% and 8.9% of tumors were positive, respectively. Furthermore, a statistically significant association between the expression of both proteins and various clinicopathological parameters was not found, except for the number of pregnancies that seems to be associated with SIX1 positivity (p = 0.034). Regarding the relationship between levels of miRNAs and expression of their putative targets, there was no statistically association. In the future a bigger sample size should be used to increase the robustness of results and patient’s follow-up would allow evaluating the association between SIX1 and SOX2 with therapeutic outcome.Rodrigues, AntónioMartins, ManuelaGomes, BrunoRUNSantos, Bruno Filipe da Silva2014-12-15T17:42:50Z2014-112014-122014-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/13914enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:48:48Zoai:run.unl.pt:10362/13914Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:21:30.125499Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Immunohistochemistry detection of putative miR-200c and miR-203 targets in breast cancer patients
title Immunohistochemistry detection of putative miR-200c and miR-203 targets in breast cancer patients
spellingShingle Immunohistochemistry detection of putative miR-200c and miR-203 targets in breast cancer patients
Santos, Bruno Filipe da Silva
ATM
BMI1
Breast cancer
miR-200c
miR-203
SIX1
title_short Immunohistochemistry detection of putative miR-200c and miR-203 targets in breast cancer patients
title_full Immunohistochemistry detection of putative miR-200c and miR-203 targets in breast cancer patients
title_fullStr Immunohistochemistry detection of putative miR-200c and miR-203 targets in breast cancer patients
title_full_unstemmed Immunohistochemistry detection of putative miR-200c and miR-203 targets in breast cancer patients
title_sort Immunohistochemistry detection of putative miR-200c and miR-203 targets in breast cancer patients
author Santos, Bruno Filipe da Silva
author_facet Santos, Bruno Filipe da Silva
author_role author
dc.contributor.none.fl_str_mv Rodrigues, António
Martins, Manuela
Gomes, Bruno
RUN
dc.contributor.author.fl_str_mv Santos, Bruno Filipe da Silva
dc.subject.por.fl_str_mv ATM
BMI1
Breast cancer
miR-200c
miR-203
SIX1
topic ATM
BMI1
Breast cancer
miR-200c
miR-203
SIX1
description Part of this thesis will be published in the following: Gomes, B.C., Santos, B. 2015. Methods for studying microRNAs expression and their targets in formalin-fixed, paraffin-embedded (FFPE) breast cancer tissues. In Methods in Molecular Biology: Cancer Drug Resistance (Rueff, J. & Rodrigues, A.S. eds), Springer Protocols.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-15T17:42:50Z
2014-11
2014-12
2014-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/13914
url http://hdl.handle.net/10362/13914
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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