Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nervous systems-regenerative medicine: effects on neuron/glial cell viability and internalization efficiency
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/20648 |
Resumo: | The applicability of CMCht/PAMAM dendrimer nanoparticles for CNS applications was investigated. AFM and TEM observations revealed that the nanoparticles possessed a nanosphere- like shape with a size from 22.0 to 30.7 nm. The nanoparticles could be bound to fluorescent-probe FITC for tracing purposes. Post-natal hippocampal neurons and cortical glial cells were both able to internalize the FITC-labeled CMCht/PAMAM dendrimer nanoparticles with high efficiency. The percentage of positive cells internalizing the nanoparticles varied, reaching a peak after 48 h of incubation. Further experiments for periods up to 7 d revealed that the periodical addition of FITC-labelled CMCht/ PAMAM dendrimer nanoparticles was needed to maintain the overall percentage of cells internalizing them. Finally, it was also observed that cell viability was not significantly affected by the incubation of dendrimer nanoparticles. |
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Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nervous systems-regenerative medicine: effects on neuron/glial cell viability and internalization efficiencyCell viabilityDendrimersGlial cellsInternalizationIntracellular drug delivery systemsScience & TechnologyThe applicability of CMCht/PAMAM dendrimer nanoparticles for CNS applications was investigated. AFM and TEM observations revealed that the nanoparticles possessed a nanosphere- like shape with a size from 22.0 to 30.7 nm. The nanoparticles could be bound to fluorescent-probe FITC for tracing purposes. Post-natal hippocampal neurons and cortical glial cells were both able to internalize the FITC-labeled CMCht/PAMAM dendrimer nanoparticles with high efficiency. The percentage of positive cells internalizing the nanoparticles varied, reaching a peak after 48 h of incubation. Further experiments for periods up to 7 d revealed that the periodical addition of FITC-labelled CMCht/ PAMAM dendrimer nanoparticles was needed to maintain the overall percentage of cells internalizing them. Finally, it was also observed that cell viability was not significantly affected by the incubation of dendrimer nanoparticles.Financial support from the Portuguese Foundation for Science and Technology through funds from POCTI and/or FEDER programs (Funding to ICVS, 3B's Research Group, post-doctoral and pre-doctoral fellowships to A.J. Salgado and J.M. Oliveira - SFRH/BPD/17595/2004; SFRH/BD/21786/2005) is gratefully acknowledged. This work was also carried out under the scope of the European NoE EXPERTISSUES (NMP3-CT-2004-500283) and HIPPOCRATES STREP (NMP3-CT-2003-505758) projects.Wiley-VCH VerlagUniversidade do MinhoSalgado, A. J.Oliveira, Joaquim M.Pirraco, RogérioPereira, Vitor H.Fraga, J. S.Marques, A. P.Neves, N. M.Mano, J. F.Reis, R. L.Sousa, Nuno2010-102010-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20648eng1616-518710.1002/mabi.20100000520602413http://onlinelibrary.wiley.com/doi/10.1002/mabi.201000005/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:11:30Zoai:repositorium.sdum.uminho.pt:1822/20648Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:03:17.113972Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nervous systems-regenerative medicine: effects on neuron/glial cell viability and internalization efficiency |
title |
Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nervous systems-regenerative medicine: effects on neuron/glial cell viability and internalization efficiency |
spellingShingle |
Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nervous systems-regenerative medicine: effects on neuron/glial cell viability and internalization efficiency Salgado, A. J. Cell viability Dendrimers Glial cells Internalization Intracellular drug delivery systems Science & Technology |
title_short |
Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nervous systems-regenerative medicine: effects on neuron/glial cell viability and internalization efficiency |
title_full |
Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nervous systems-regenerative medicine: effects on neuron/glial cell viability and internalization efficiency |
title_fullStr |
Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nervous systems-regenerative medicine: effects on neuron/glial cell viability and internalization efficiency |
title_full_unstemmed |
Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nervous systems-regenerative medicine: effects on neuron/glial cell viability and internalization efficiency |
title_sort |
Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nervous systems-regenerative medicine: effects on neuron/glial cell viability and internalization efficiency |
author |
Salgado, A. J. |
author_facet |
Salgado, A. J. Oliveira, Joaquim M. Pirraco, Rogério Pereira, Vitor H. Fraga, J. S. Marques, A. P. Neves, N. M. Mano, J. F. Reis, R. L. Sousa, Nuno |
author_role |
author |
author2 |
Oliveira, Joaquim M. Pirraco, Rogério Pereira, Vitor H. Fraga, J. S. Marques, A. P. Neves, N. M. Mano, J. F. Reis, R. L. Sousa, Nuno |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Salgado, A. J. Oliveira, Joaquim M. Pirraco, Rogério Pereira, Vitor H. Fraga, J. S. Marques, A. P. Neves, N. M. Mano, J. F. Reis, R. L. Sousa, Nuno |
dc.subject.por.fl_str_mv |
Cell viability Dendrimers Glial cells Internalization Intracellular drug delivery systems Science & Technology |
topic |
Cell viability Dendrimers Glial cells Internalization Intracellular drug delivery systems Science & Technology |
description |
The applicability of CMCht/PAMAM dendrimer nanoparticles for CNS applications was investigated. AFM and TEM observations revealed that the nanoparticles possessed a nanosphere- like shape with a size from 22.0 to 30.7 nm. The nanoparticles could be bound to fluorescent-probe FITC for tracing purposes. Post-natal hippocampal neurons and cortical glial cells were both able to internalize the FITC-labeled CMCht/PAMAM dendrimer nanoparticles with high efficiency. The percentage of positive cells internalizing the nanoparticles varied, reaching a peak after 48 h of incubation. Further experiments for periods up to 7 d revealed that the periodical addition of FITC-labelled CMCht/ PAMAM dendrimer nanoparticles was needed to maintain the overall percentage of cells internalizing them. Finally, it was also observed that cell viability was not significantly affected by the incubation of dendrimer nanoparticles. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10 2010-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/20648 |
url |
http://hdl.handle.net/1822/20648 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1616-5187 10.1002/mabi.201000005 20602413 http://onlinelibrary.wiley.com/doi/10.1002/mabi.201000005/pdf |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-VCH Verlag |
publisher.none.fl_str_mv |
Wiley-VCH Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132437978021888 |