Structural basis for energy transduction by respiratory alternative complex III
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1038/s41467-018-04141-8 |
Resumo: | Electron transfer in respiratory chains generates the electrochemical potential that serves as energy source for the cell. Prokaryotes can use a wide range of electron donors and acceptors and may have alternative complexes performing the same catalytic reactions as the mitochondrial complexes. This is the case for the alternative complex III (ACIII), a quinol:cytochrome c/HiPIP oxidoreductase. In order to understand the catalytic mechanism of this respiratory enzyme, we determined the structure of ACIII from Rhodothermus marinus at 3.9 Å resolution by single-particle cryo-electron microscopy. ACIII presents a so-far unique structure, for which we establish the arrangement of the cofactors (four iron-sulfur clusters and six c-type hemes) and propose the location of the quinol-binding site and the presence of two putative proton pathways in the membrane. Altogether, this structure provides insights into a mechanism for energy transduction and introduces ACIII as a redox-driven proton pump. |
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Structural basis for energy transduction by respiratory alternative complex IIIChemistry(all)Biochemistry, Genetics and Molecular Biology(all)Physics and Astronomy(all)Electron transfer in respiratory chains generates the electrochemical potential that serves as energy source for the cell. Prokaryotes can use a wide range of electron donors and acceptors and may have alternative complexes performing the same catalytic reactions as the mitochondrial complexes. This is the case for the alternative complex III (ACIII), a quinol:cytochrome c/HiPIP oxidoreductase. In order to understand the catalytic mechanism of this respiratory enzyme, we determined the structure of ACIII from Rhodothermus marinus at 3.9 Å resolution by single-particle cryo-electron microscopy. ACIII presents a so-far unique structure, for which we establish the arrangement of the cofactors (four iron-sulfur clusters and six c-type hemes) and propose the location of the quinol-binding site and the presence of two putative proton pathways in the membrane. Altogether, this structure provides insights into a mechanism for energy transduction and introduces ACIII as a redox-driven proton pump.Molecular, Structural and Cellular Microbiology (MOSTMICRO)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNSousa, Joana S.Calisto, FilipaLanger, Julian D.Mills, Deryck J.Refojo, Patrícia N.Teixeira, MiguelKühlbrandt, WernerVonck, JanetPereira, Manuela M.2019-04-29T22:15:10Z2018-12-012018-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1038/s41467-018-04141-8eng2041-1723PURE: 6322796http://www.scopus.com/inward/record.url?scp=85046338159&partnerID=8YFLogxKhttps://doi.org/10.1038/s41467-018-04141-8info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:32:08Zoai:run.unl.pt:10362/68101Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:34:42.327699Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Structural basis for energy transduction by respiratory alternative complex III |
title |
Structural basis for energy transduction by respiratory alternative complex III |
spellingShingle |
Structural basis for energy transduction by respiratory alternative complex III Sousa, Joana S. Chemistry(all) Biochemistry, Genetics and Molecular Biology(all) Physics and Astronomy(all) |
title_short |
Structural basis for energy transduction by respiratory alternative complex III |
title_full |
Structural basis for energy transduction by respiratory alternative complex III |
title_fullStr |
Structural basis for energy transduction by respiratory alternative complex III |
title_full_unstemmed |
Structural basis for energy transduction by respiratory alternative complex III |
title_sort |
Structural basis for energy transduction by respiratory alternative complex III |
author |
Sousa, Joana S. |
author_facet |
Sousa, Joana S. Calisto, Filipa Langer, Julian D. Mills, Deryck J. Refojo, Patrícia N. Teixeira, Miguel Kühlbrandt, Werner Vonck, Janet Pereira, Manuela M. |
author_role |
author |
author2 |
Calisto, Filipa Langer, Julian D. Mills, Deryck J. Refojo, Patrícia N. Teixeira, Miguel Kühlbrandt, Werner Vonck, Janet Pereira, Manuela M. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Molecular, Structural and Cellular Microbiology (MOSTMICRO) Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
dc.contributor.author.fl_str_mv |
Sousa, Joana S. Calisto, Filipa Langer, Julian D. Mills, Deryck J. Refojo, Patrícia N. Teixeira, Miguel Kühlbrandt, Werner Vonck, Janet Pereira, Manuela M. |
dc.subject.por.fl_str_mv |
Chemistry(all) Biochemistry, Genetics and Molecular Biology(all) Physics and Astronomy(all) |
topic |
Chemistry(all) Biochemistry, Genetics and Molecular Biology(all) Physics and Astronomy(all) |
description |
Electron transfer in respiratory chains generates the electrochemical potential that serves as energy source for the cell. Prokaryotes can use a wide range of electron donors and acceptors and may have alternative complexes performing the same catalytic reactions as the mitochondrial complexes. This is the case for the alternative complex III (ACIII), a quinol:cytochrome c/HiPIP oxidoreductase. In order to understand the catalytic mechanism of this respiratory enzyme, we determined the structure of ACIII from Rhodothermus marinus at 3.9 Å resolution by single-particle cryo-electron microscopy. ACIII presents a so-far unique structure, for which we establish the arrangement of the cofactors (four iron-sulfur clusters and six c-type hemes) and propose the location of the quinol-binding site and the presence of two putative proton pathways in the membrane. Altogether, this structure provides insights into a mechanism for energy transduction and introduces ACIII as a redox-driven proton pump. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 2018-12-01T00:00:00Z 2019-04-29T22:15:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1038/s41467-018-04141-8 |
url |
https://doi.org/10.1038/s41467-018-04141-8 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2041-1723 PURE: 6322796 http://www.scopus.com/inward/record.url?scp=85046338159&partnerID=8YFLogxK https://doi.org/10.1038/s41467-018-04141-8 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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