Self-assembled nanoparticles as new smart contrast agents for magnetic resonance imaging

Detalhes bibliográficos
Autor(a) principal: Simão, Teresa
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/1735
Resumo: Dissertação de mest., Ciências Biomédicas, Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2010
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spelling Self-assembled nanoparticles as new smart contrast agents for magnetic resonance imagingAgente de contraste inteligenteImagiologia médicaNanopartículasPolímeros dibloco anfifílicosMucina-1Dissertação de mest., Ciências Biomédicas, Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2010The aim of this thesis is to develop smart and targeted nanoparticle contrast agents for Magnetic Resonance Imaging. These nanoparticles were designed to improve the sensitivity of this high resolution imaging technique and thereby improve early cancer detection, which is a major factor for the reduction of cancer mortality. The designed nanoparticles are expected to accumulate in the tumor through passive and active targeting. In addition, when exposed to the characteristic low pH of the cancer microenvironment, the nanoparticles will release the contrast agent, which will turn on its imaging capability. This contrast agent consists of pH-sensitive polymeric micelles formed by self-assembly, loaded with Gadolinium (III) complexes and bioconjugated with the C595 monoclonal antibody against the human MUC1 protein, which is overexpressed during tumorigenesis since its early stages. The capability of micelles to disintegrate and release the encapsulated contrast in acidic conditions was proved by 1.5T MRI experiments. The MRI study showed no image signal from the sample with intact micelles whereas a signal enhancement was observed from the sample at low pH. After one mouse has been intramuscularly injected with Gadolinium(III)-loaded micelles in both hind legs, the MR image demonstrated a stronger signal from the right hind leg, which was previously injected with an acid solution. To assess in vitro cytotoxicity of free and encapsulated Gadolinium (III) complexes in polymeric micelles, MTT assays were performed on different cancer cell lines. Encapsulated Gadolinium (III) complexes showed significantly lower cytotoxicity than free Gadolinium (III) complexes, even at the highest concentration. To verify the target capability of nanoparticles, fluorescent dye-loaded polymeric micelles were incubated with breast cancer cells expressing MUC1 (verified by semi-quantitative RT-PCR, Western blotting and flow cytometry) and mouse bone marrow stroma cells. Antibody-conjugated micelles had superior affinity for MUC1-expressing human breast cancer cells than for mouse bone marrow cells.Mouffouk, FouziSantos, Nuno Rodrigues dosSapientiaSimão, Teresa2012-10-11T17:57:26Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.1/1735eng615.8 SIM*Sel Caveinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:12:53Zoai:sapientia.ualg.pt:10400.1/1735Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:55:51.924628Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Self-assembled nanoparticles as new smart contrast agents for magnetic resonance imaging
title Self-assembled nanoparticles as new smart contrast agents for magnetic resonance imaging
spellingShingle Self-assembled nanoparticles as new smart contrast agents for magnetic resonance imaging
Simão, Teresa
Agente de contraste inteligente
Imagiologia médica
Nanopartículas
Polímeros dibloco anfifílicos
Mucina-1
title_short Self-assembled nanoparticles as new smart contrast agents for magnetic resonance imaging
title_full Self-assembled nanoparticles as new smart contrast agents for magnetic resonance imaging
title_fullStr Self-assembled nanoparticles as new smart contrast agents for magnetic resonance imaging
title_full_unstemmed Self-assembled nanoparticles as new smart contrast agents for magnetic resonance imaging
title_sort Self-assembled nanoparticles as new smart contrast agents for magnetic resonance imaging
author Simão, Teresa
author_facet Simão, Teresa
author_role author
dc.contributor.none.fl_str_mv Mouffouk, Fouzi
Santos, Nuno Rodrigues dos
Sapientia
dc.contributor.author.fl_str_mv Simão, Teresa
dc.subject.por.fl_str_mv Agente de contraste inteligente
Imagiologia médica
Nanopartículas
Polímeros dibloco anfifílicos
Mucina-1
topic Agente de contraste inteligente
Imagiologia médica
Nanopartículas
Polímeros dibloco anfifílicos
Mucina-1
description Dissertação de mest., Ciências Biomédicas, Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2010
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
2012-10-11T17:57:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/1735
url http://hdl.handle.net/10400.1/1735
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 615.8 SIM*Sel Cave
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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