Evaluation of Nanoparticle Uptake iii Co-culture Cancer Modeis

Detalhes bibliográficos
Autor(a) principal: Costa, C.E.
Data de Publicação: 2013
Outros Autores: Gaspar, V.M., Marques, J.G., Coutinho, Paula, Correia, I. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10314/2513
Resumo: Co-culture models are currently bridging the gap between classical cultures and in vivo animal models. Exploring this novel approach unlocks the possibility to mimic the tumor microenvironment in vitro, through the establishment of cancer-stroma synergistic interactions. Notably, these organotypic models offer a perfect platform for the development and pre-clinical evaluation of candidate nanocarriers loaded with anti-tumoral drugs in a high throughput screening mode, with lower costs and absence of ethical issues. However, this evaluation was until now limited to co-culture systems established with precise cell ratios, not addressing the natural cell heterogeneity commonly found in different tumors. Therefore, herein the multifunctional nanocarriers efficiency was characterized in various fibroblast-MCF-7 co-culture systems containing different cell ratios, in order to unravel key design parameters that influence nanocarrier performance and the therapeutic outcome. The successful establishment of the co-culture models was confirmed by the tissue-like distribution of the different cells in culture. Nanoparticles incubation in the various co-culture systems reveals that these nanocarriers possess targeting specificity for cancer cells, indicating their suitability for being used in this illness therapy. Additionally, by using different co-culture ratios, different nanoparticle uptake profiles were obtained. These findings are of crucial importance for the future design and optimization of new drug delivery systems, since their real targeting capacity must be addressed in heterogenous cell populations, such as those found in tumors.
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spelling Evaluation of Nanoparticle Uptake iii Co-culture Cancer ModeisCo-culture models are currently bridging the gap between classical cultures and in vivo animal models. Exploring this novel approach unlocks the possibility to mimic the tumor microenvironment in vitro, through the establishment of cancer-stroma synergistic interactions. Notably, these organotypic models offer a perfect platform for the development and pre-clinical evaluation of candidate nanocarriers loaded with anti-tumoral drugs in a high throughput screening mode, with lower costs and absence of ethical issues. However, this evaluation was until now limited to co-culture systems established with precise cell ratios, not addressing the natural cell heterogeneity commonly found in different tumors. Therefore, herein the multifunctional nanocarriers efficiency was characterized in various fibroblast-MCF-7 co-culture systems containing different cell ratios, in order to unravel key design parameters that influence nanocarrier performance and the therapeutic outcome. The successful establishment of the co-culture models was confirmed by the tissue-like distribution of the different cells in culture. Nanoparticles incubation in the various co-culture systems reveals that these nanocarriers possess targeting specificity for cancer cells, indicating their suitability for being used in this illness therapy. Additionally, by using different co-culture ratios, different nanoparticle uptake profiles were obtained. These findings are of crucial importance for the future design and optimization of new drug delivery systems, since their real targeting capacity must be addressed in heterogenous cell populations, such as those found in tumors.PEst-OE/EGEJUI4D5G/2011Michiya Maiauaaki, Osaka University, Japan2016-08-022013-07-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10314/2513http://hdl.handle.net/10314/2513engCoata EC. Gaspar VM. Marques JG, Coilinho P, Correia 012013) Evaluatio, orNanoparticie Uprate in Co’ruftere Canrer ModaIs, rros C’JE 5(71; e70072.Costa, C.E.Gaspar, V.M.Marques, J.G.Coutinho, PaulaCorreia, I. J.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-14T02:55:28Zoai:bdigital.ipg.pt:10314/2513Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:42:05.228309Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of Nanoparticle Uptake iii Co-culture Cancer Modeis
title Evaluation of Nanoparticle Uptake iii Co-culture Cancer Modeis
spellingShingle Evaluation of Nanoparticle Uptake iii Co-culture Cancer Modeis
Costa, C.E.
title_short Evaluation of Nanoparticle Uptake iii Co-culture Cancer Modeis
title_full Evaluation of Nanoparticle Uptake iii Co-culture Cancer Modeis
title_fullStr Evaluation of Nanoparticle Uptake iii Co-culture Cancer Modeis
title_full_unstemmed Evaluation of Nanoparticle Uptake iii Co-culture Cancer Modeis
title_sort Evaluation of Nanoparticle Uptake iii Co-culture Cancer Modeis
author Costa, C.E.
author_facet Costa, C.E.
Gaspar, V.M.
Marques, J.G.
Coutinho, Paula
Correia, I. J.
author_role author
author2 Gaspar, V.M.
Marques, J.G.
Coutinho, Paula
Correia, I. J.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Costa, C.E.
Gaspar, V.M.
Marques, J.G.
Coutinho, Paula
Correia, I. J.
description Co-culture models are currently bridging the gap between classical cultures and in vivo animal models. Exploring this novel approach unlocks the possibility to mimic the tumor microenvironment in vitro, through the establishment of cancer-stroma synergistic interactions. Notably, these organotypic models offer a perfect platform for the development and pre-clinical evaluation of candidate nanocarriers loaded with anti-tumoral drugs in a high throughput screening mode, with lower costs and absence of ethical issues. However, this evaluation was until now limited to co-culture systems established with precise cell ratios, not addressing the natural cell heterogeneity commonly found in different tumors. Therefore, herein the multifunctional nanocarriers efficiency was characterized in various fibroblast-MCF-7 co-culture systems containing different cell ratios, in order to unravel key design parameters that influence nanocarrier performance and the therapeutic outcome. The successful establishment of the co-culture models was confirmed by the tissue-like distribution of the different cells in culture. Nanoparticles incubation in the various co-culture systems reveals that these nanocarriers possess targeting specificity for cancer cells, indicating their suitability for being used in this illness therapy. Additionally, by using different co-culture ratios, different nanoparticle uptake profiles were obtained. These findings are of crucial importance for the future design and optimization of new drug delivery systems, since their real targeting capacity must be addressed in heterogenous cell populations, such as those found in tumors.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-26T00:00:00Z
2016-08-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10314/2513
http://hdl.handle.net/10314/2513
url http://hdl.handle.net/10314/2513
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Coata EC. Gaspar VM. Marques JG, Coilinho P, Correia 012013) Evaluatio, orNanoparticie Uprate in Co’ruftere Canrer ModaIs, rros C’JE 5(71; e70072.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Michiya Maiauaaki, Osaka University, Japan
publisher.none.fl_str_mv Michiya Maiauaaki, Osaka University, Japan
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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