Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry

Detalhes bibliográficos
Autor(a) principal: Quini, Caio C. [UNESP]
Data de Publicação: 2017
Outros Autores: Prospero, Andre G. [UNESP], Calabresi, Marcos F. F. [UNESP], Moretto, Gustavo M. [UNESP], Zufelato, Nicholas, Krishnan, Sunil, Pina, Diana R. [UNESP], Oliveira, Ricardo B., Baffa, Oswaldo, Bakuzis, Andris F., Miranda, Jose R. A. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.nano.2017.02.005
http://hdl.handle.net/11449/162855
Resumo: We describe the development of a joint in vivo/ex vivo protocol to monitor magnetic nanoparticles in animal models. Alternating current biosusceptometry (ACB) enables the assessment of magnetic nanoparticle accumulation, followed by quantitative analysis of concentrations in organs of interest. We present a study of real-time liver accumulation, followed by the assessment of sequential biodistribution using the same technique. For quantification, we validated our results by comparing all of the data with electron spin resonance (ESR). The ACB had viable temporal resolution and accuracy to differentiate temporal parameters of liver accumulation, caused by vasculature extravasation and macrophages action. The biodistribution experiment showed different uptake profiles for different doses and injection protocols. Comparisons with the ESR system indicated a correlation index of 0.993. We present the ACB system as an accessible and versatile tool to monitor magnetic nanoparticles, allowing in vivo and real-time evaluations of distribution and quantitative assessments of particle concentrations. (C) 2017 Elsevier Inc. All rights reserved.
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spelling Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometryAC biosusceptometryMagnetic nanoparticlesBiomedical instrumentsReal time monitoringQuantification methodsNanoparticle uptakeWe describe the development of a joint in vivo/ex vivo protocol to monitor magnetic nanoparticles in animal models. Alternating current biosusceptometry (ACB) enables the assessment of magnetic nanoparticle accumulation, followed by quantitative analysis of concentrations in organs of interest. We present a study of real-time liver accumulation, followed by the assessment of sequential biodistribution using the same technique. For quantification, we validated our results by comparing all of the data with electron spin resonance (ESR). The ACB had viable temporal resolution and accuracy to differentiate temporal parameters of liver accumulation, caused by vasculature extravasation and macrophages action. The biodistribution experiment showed different uptake profiles for different doses and injection protocols. Comparisons with the ESR system indicated a correlation index of 0.993. We present the ACB system as an accessible and versatile tool to monitor magnetic nanoparticles, allowing in vivo and real-time evaluations of distribution and quantitative assessments of particle concentrations. (C) 2017 Elsevier Inc. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Fis & Biofis, Lab Biomagnetismo, IBB, Botucatu, SP, BrazilUniv Fed Goias, Inst Fis, Goiania, Go, BrazilUniv Texas MD Anderson Canc Ctr, Dept Expt Radiat Oncol, Houston, TX 77030 USAUniv Estadual Paulista, Fac Med Botucatu, Dept Doencas Trop & Diagnost Imagem, Botucatu, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Dept Fis & Biofis, Lab Biomagnetismo, IBB, Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med Botucatu, Dept Doencas Trop & Diagnost Imagem, Botucatu, SP, BrazilFAPESP: 2010/07639-9FAPESP: 2011/18696-6FAPESP: 2013/20842-6FAPESP: 2015/14914-0Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Federal de Goiás (UFG)Univ Texas MD Anderson Canc CtrUniversidade de São Paulo (USP)Quini, Caio C. [UNESP]Prospero, Andre G. [UNESP]Calabresi, Marcos F. F. [UNESP]Moretto, Gustavo M. [UNESP]Zufelato, NicholasKrishnan, SunilPina, Diana R. [UNESP]Oliveira, Ricardo B.Baffa, OswaldoBakuzis, Andris F.Miranda, Jose R. A. [UNESP]2018-11-26T17:34:41Z2018-11-26T17:34:41Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1519-1529application/pdfhttp://dx.doi.org/10.1016/j.nano.2017.02.005Nanomedicine-nanotechnology Biology And Medicine. Amsterdam: Elsevier Science Bv, v. 13, n. 4, p. 1519-1529, 2017.1549-9634http://hdl.handle.net/11449/16285510.1016/j.nano.2017.02.005WOS:000402678800019WOS000402678800019.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNanomedicine-nanotechnology Biology And Medicine1,743info:eu-repo/semantics/openAccess2024-08-15T15:22:34Zoai:repositorio.unesp.br:11449/162855Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:22:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry
title Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry
spellingShingle Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry
Quini, Caio C. [UNESP]
AC biosusceptometry
Magnetic nanoparticles
Biomedical instruments
Real time monitoring
Quantification methods
Nanoparticle uptake
title_short Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry
title_full Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry
title_fullStr Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry
title_full_unstemmed Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry
title_sort Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry
author Quini, Caio C. [UNESP]
author_facet Quini, Caio C. [UNESP]
Prospero, Andre G. [UNESP]
Calabresi, Marcos F. F. [UNESP]
Moretto, Gustavo M. [UNESP]
Zufelato, Nicholas
Krishnan, Sunil
Pina, Diana R. [UNESP]
Oliveira, Ricardo B.
Baffa, Oswaldo
Bakuzis, Andris F.
Miranda, Jose R. A. [UNESP]
author_role author
author2 Prospero, Andre G. [UNESP]
Calabresi, Marcos F. F. [UNESP]
Moretto, Gustavo M. [UNESP]
Zufelato, Nicholas
Krishnan, Sunil
Pina, Diana R. [UNESP]
Oliveira, Ricardo B.
Baffa, Oswaldo
Bakuzis, Andris F.
Miranda, Jose R. A. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Goiás (UFG)
Univ Texas MD Anderson Canc Ctr
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Quini, Caio C. [UNESP]
Prospero, Andre G. [UNESP]
Calabresi, Marcos F. F. [UNESP]
Moretto, Gustavo M. [UNESP]
Zufelato, Nicholas
Krishnan, Sunil
Pina, Diana R. [UNESP]
Oliveira, Ricardo B.
Baffa, Oswaldo
Bakuzis, Andris F.
Miranda, Jose R. A. [UNESP]
dc.subject.por.fl_str_mv AC biosusceptometry
Magnetic nanoparticles
Biomedical instruments
Real time monitoring
Quantification methods
Nanoparticle uptake
topic AC biosusceptometry
Magnetic nanoparticles
Biomedical instruments
Real time monitoring
Quantification methods
Nanoparticle uptake
description We describe the development of a joint in vivo/ex vivo protocol to monitor magnetic nanoparticles in animal models. Alternating current biosusceptometry (ACB) enables the assessment of magnetic nanoparticle accumulation, followed by quantitative analysis of concentrations in organs of interest. We present a study of real-time liver accumulation, followed by the assessment of sequential biodistribution using the same technique. For quantification, we validated our results by comparing all of the data with electron spin resonance (ESR). The ACB had viable temporal resolution and accuracy to differentiate temporal parameters of liver accumulation, caused by vasculature extravasation and macrophages action. The biodistribution experiment showed different uptake profiles for different doses and injection protocols. Comparisons with the ESR system indicated a correlation index of 0.993. We present the ACB system as an accessible and versatile tool to monitor magnetic nanoparticles, allowing in vivo and real-time evaluations of distribution and quantitative assessments of particle concentrations. (C) 2017 Elsevier Inc. All rights reserved.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-01
2018-11-26T17:34:41Z
2018-11-26T17:34:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.nano.2017.02.005
Nanomedicine-nanotechnology Biology And Medicine. Amsterdam: Elsevier Science Bv, v. 13, n. 4, p. 1519-1529, 2017.
1549-9634
http://hdl.handle.net/11449/162855
10.1016/j.nano.2017.02.005
WOS:000402678800019
WOS000402678800019.pdf
url http://dx.doi.org/10.1016/j.nano.2017.02.005
http://hdl.handle.net/11449/162855
identifier_str_mv Nanomedicine-nanotechnology Biology And Medicine. Amsterdam: Elsevier Science Bv, v. 13, n. 4, p. 1519-1529, 2017.
1549-9634
10.1016/j.nano.2017.02.005
WOS:000402678800019
WOS000402678800019.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nanomedicine-nanotechnology Biology And Medicine
1,743
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1519-1529
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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