Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections

Detalhes bibliográficos
Autor(a) principal: Dardouri, Maïssa
Data de Publicação: 2022
Outros Autores: Aljnadi, Israa M., Deuermeier, Jonas, Santos, Catarina, Costa, Fabiola, Martin, Victor, Fernandes, Maria H., Gonçalves, Lídia, Bettencourt, Ana, Gomes, Pedro Sousa, Ribeiro, Isabel A. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/59212
Resumo: In clinic there is a demand to solve the drawback of medical devices multispecies related infections. Consequently, different biomaterial surfaces, such as vascular catheters, urgently need improvement regarding their antifouling/antimicrobial properties. In this work, we covalently functionalized medical grade polydimethylsiloxane (PDMS) with antimicrobial rhamnolipids to investigate the biomaterial surface activity towards mono and dual species biofilms. Preparation of surfaces with “piranha” oxidation, followed by APTES bonding and carbodiimide reaction with rhamnolipids effectively bonded these compounds to PDMS surface as confirmed by FTIR-ATR and XPS analysis. Generated surfaces were active towards S. aureus biofilm formation showing a 4.2 log reduction while with S. epidermidis and C. albicans biofilms a reduction of 1.2 and 1.0 log reduction, respectively, was observed. Regarding dual-species testing the higher biofilm log reduction observed was 1.9. Additionally, biocompatibility was assessed by cytocompatibility towards human fibroblastic cells, low platelet activation and absence of vascular irritation. Our work not only sheds light on using covalently bonded rhamnolipids towards dual species biofilms but also highlights the biocompatibility of the obtained PDMS surfaces.
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spelling Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infectionsAntimicrobialsMedical devicesPDMSAntibiofilmRhamnolipidsBiosurfactantsStaphylococcus aureusCandida albicansStaphylococcus epidermidisIn clinic there is a demand to solve the drawback of medical devices multispecies related infections. Consequently, different biomaterial surfaces, such as vascular catheters, urgently need improvement regarding their antifouling/antimicrobial properties. In this work, we covalently functionalized medical grade polydimethylsiloxane (PDMS) with antimicrobial rhamnolipids to investigate the biomaterial surface activity towards mono and dual species biofilms. Preparation of surfaces with “piranha” oxidation, followed by APTES bonding and carbodiimide reaction with rhamnolipids effectively bonded these compounds to PDMS surface as confirmed by FTIR-ATR and XPS analysis. Generated surfaces were active towards S. aureus biofilm formation showing a 4.2 log reduction while with S. epidermidis and C. albicans biofilms a reduction of 1.2 and 1.0 log reduction, respectively, was observed. Regarding dual-species testing the higher biofilm log reduction observed was 1.9. Additionally, biocompatibility was assessed by cytocompatibility towards human fibroblastic cells, low platelet activation and absence of vascular irritation. Our work not only sheds light on using covalently bonded rhamnolipids towards dual species biofilms but also highlights the biocompatibility of the obtained PDMS surfaces.The authors thank Fundação para a Ciência e Tecnologia (FCT) Portugal, for the financial support under the Project PTDC/BTM-SAL/29335/2017. Portugal 2020 for the Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa RNEM) is also acknowledged. The authors also acknowledge the Designer Nuno Monge for the artwork, Isabel Dias Nogueira (Microlab, Instituto Superior Técnico, Lisbon University) for the technical assistance with the SEM analysis, Andreia Bento for the assistance with the UHPLC-MS equipment (iMed.Ulisboa) and Professor Maria José Ferreira (FFULisboa) for allowing the use of the automatic flash chromatography equipment.ElsevierRepositório da Universidade de LisboaDardouri, MaïssaAljnadi, Israa M.Deuermeier, JonasSantos, CatarinaCosta, FabiolaMartin, VictorFernandes, Maria H.Gonçalves, LídiaBettencourt, AnaGomes, Pedro SousaRibeiro, Isabel A. C.2023-09-11T17:20:22Z2022-092023-02-27T14:54:38Z2022-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/59212engDardouri M, Aljnadi IM, Deuermeier J, Santos C, Costa F, Martin V, et al. Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections. Colloids and Surfaces B: Biointerfaces [Internet]. 1 de setembro de 2022;217:112679. Disponível em: https://www.sciencedirect.com/science/article/pii/S09277765220036290927-7765cv-prod-314758910.1016/j.colsurfb.2022.112679info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:04:03Zoai:repositorio.ul.pt:10451/59212Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:06:59.416656Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections
title Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections
spellingShingle Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections
Dardouri, Maïssa
Antimicrobials
Medical devices
PDMS
Antibiofilm
Rhamnolipids
Biosurfactants
Staphylococcus aureus
Candida albicans
Staphylococcus epidermidis
title_short Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections
title_full Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections
title_fullStr Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections
title_full_unstemmed Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections
title_sort Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections
author Dardouri, Maïssa
author_facet Dardouri, Maïssa
Aljnadi, Israa M.
Deuermeier, Jonas
Santos, Catarina
Costa, Fabiola
Martin, Victor
Fernandes, Maria H.
Gonçalves, Lídia
Bettencourt, Ana
Gomes, Pedro Sousa
Ribeiro, Isabel A. C.
author_role author
author2 Aljnadi, Israa M.
Deuermeier, Jonas
Santos, Catarina
Costa, Fabiola
Martin, Victor
Fernandes, Maria H.
Gonçalves, Lídia
Bettencourt, Ana
Gomes, Pedro Sousa
Ribeiro, Isabel A. C.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Dardouri, Maïssa
Aljnadi, Israa M.
Deuermeier, Jonas
Santos, Catarina
Costa, Fabiola
Martin, Victor
Fernandes, Maria H.
Gonçalves, Lídia
Bettencourt, Ana
Gomes, Pedro Sousa
Ribeiro, Isabel A. C.
dc.subject.por.fl_str_mv Antimicrobials
Medical devices
PDMS
Antibiofilm
Rhamnolipids
Biosurfactants
Staphylococcus aureus
Candida albicans
Staphylococcus epidermidis
topic Antimicrobials
Medical devices
PDMS
Antibiofilm
Rhamnolipids
Biosurfactants
Staphylococcus aureus
Candida albicans
Staphylococcus epidermidis
description In clinic there is a demand to solve the drawback of medical devices multispecies related infections. Consequently, different biomaterial surfaces, such as vascular catheters, urgently need improvement regarding their antifouling/antimicrobial properties. In this work, we covalently functionalized medical grade polydimethylsiloxane (PDMS) with antimicrobial rhamnolipids to investigate the biomaterial surface activity towards mono and dual species biofilms. Preparation of surfaces with “piranha” oxidation, followed by APTES bonding and carbodiimide reaction with rhamnolipids effectively bonded these compounds to PDMS surface as confirmed by FTIR-ATR and XPS analysis. Generated surfaces were active towards S. aureus biofilm formation showing a 4.2 log reduction while with S. epidermidis and C. albicans biofilms a reduction of 1.2 and 1.0 log reduction, respectively, was observed. Regarding dual-species testing the higher biofilm log reduction observed was 1.9. Additionally, biocompatibility was assessed by cytocompatibility towards human fibroblastic cells, low platelet activation and absence of vascular irritation. Our work not only sheds light on using covalently bonded rhamnolipids towards dual species biofilms but also highlights the biocompatibility of the obtained PDMS surfaces.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
2022-09-01T00:00:00Z
2023-09-11T17:20:22Z
2023-02-27T14:54:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/59212
url http://hdl.handle.net/10451/59212
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Dardouri M, Aljnadi IM, Deuermeier J, Santos C, Costa F, Martin V, et al. Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections. Colloids and Surfaces B: Biointerfaces [Internet]. 1 de setembro de 2022;217:112679. Disponível em: https://www.sciencedirect.com/science/article/pii/S0927776522003629
0927-7765
cv-prod-3147589
10.1016/j.colsurfb.2022.112679
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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